LOCUS       sequence-31331-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RAB5B
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             657..674
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(727..745)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      741..788
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cctctgaata ttttgtttac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcct ggttccgcgt ggtagtgcca gcaaaatttg ccagttcaaa
      121 ttggtcctgc tgggagaatc tgcagtggga aagtcaagcc tggtattacg ttttgtcaaa
      181 gggcagttcc atgagtacca ggagagcacc attggagcgg ccttcctcac ccagtccgtt
      241 tgtctagatg acacaacagt gaagtttgag atctgggaca cagctgggca ggagcgatat
      301 cacagcttag cccccatgta ctacaggggt gcccaagctg caatcgtggt ttacgacatt
      361 actaatcagg aaacctttgc ccgagcaaag acatgggtga aggaactaca gcgacaggcc
      421 agtcctagca tcgttattgc cctggcaggg aacaaagctg acctggccaa caaacgtatg
      481 gtggagtatg aagaggccca ggcatatgca gatgacaaca gcttattgtt catggagact
      541 tcagccaaga cagctatgaa cgtgaatgat ctcttcctgg caatagctaa gaagttgcca
      601 aagagtgaac cccagaatct gggaggttga tgacgaagct tgcggccgca ctcgagcacc
      661 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      721 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      781 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
      841 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
//
