LOCUS       sequence-313316-        1458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses GABRA5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1458)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_GABRA5_OE_1
FEATURES             Location/Qualifiers
     source          1..1458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1388..1412)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1414..1455
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggagaatg gaatgttctc tggttttatc atgatcaaaa acctccttct cttttgtatt
       61 tccatgaact tatccagtca ctttggcttt tcacagatgc caaccagttc agtgaaagat
      121 gagaccaatg acaacatcac gatatttacc aggatcttgg atgggctctt ggatggctac
      181 gacaacagac ttcggcccgg gctgggagag cgcatcactc aggtgaggac cgacatctac
      241 gtcaccagct tcggcccggt gtccgacacg gaaatggagt acaccataga cgtgtttttc
      301 cgacaaagct ggaaagatga aaggcttcgg tttaaggggc ccatgcagcg cctccctctc
      361 aacaacctcc ttgccagcaa gatctggacc ccagacacgt tcttccacaa cgggaagaag
      421 tccatcgctc acaacatgac cacgcccaac aagctgctgc ggctggagga cgacggcacc
      481 ctgctctaca ccatgcgctt gaccatctct gcagagtgcc ccatgcagct tgaggacttc
      541 ccgatggatg cgcacgcttg ccctctgaaa tttggcagct atgcgtaccc taattctgaa
      601 gtcgtctacg tctggaccaa cggctccacc aagtcggtgg tggtggcgga agatggctcc
      661 agactgaacc agtaccacct gatggggcag acggtgggca ctgagaacat cagcaccagc
      721 acaggcgaat acacaatcat gacagctcac ttccacctga aaaggaagat tggctacttt
      781 gtcatccaga cctaccttcc ctgcataatg accgtgatct tatcacaggt gtccttttgg
      841 ctgaaccggg aatcagtccc agccaggaca gtttttgggg tcaccacggt gctgaccatg
      901 acgaccctca gcatcagcgc caggaactct ctgcccaaag tggcctacgc caccgccatg
      961 gactggttca tagccgtgtg ctatgccttc gtcttctcgg cgctgataga gtttgccacg
     1021 gtcaattact ttaccaagag aggctgggcc tgggatggca aaaaagcctt ggaagcagcc
     1081 aagatcaaga aaaagcgtga agtcatacta aataagtcaa caaacgcttt tacaactggg
     1141 aagatgtctc accccccaaa cattccgaag gaacagaccc cagcagggac gtcgaataca
     1201 acctcagtct cagtaaaacc ctctgaagag aagacttctg aaagcaaaaa gacttacaac
     1261 agtatcagca aaattgacaa aatgtcccga atcgtattcc cagtcttgtt cggcactttc
     1321 aacttagttt actgggcaac gtatttgaat agggagccgg tgataaaagg agccgcctct
     1381 tcaaaatacc caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc
     1441 ggtctcgatt ctacgtag
//
