LOCUS       sequence-313361-        1992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpresses SCNN1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1992)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_SCNN1B_OE_1
FEATURES             Location/Qualifiers
     source          1..1992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1922..1946)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1948..1989
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atgcacgtga agaagtacct gctgaagggc ctgcatcggc tgcagaaggg ccccggctac
       61 acgtacaagg agctgctggt gtggtactgc gacaacacca acacccacgg ccccaagcgc
      121 accatctgtg aggggcccaa gaagaaagcc atgtggttcc tgctcaccct gctcttcgcc
      181 gccctcgtct gctggcagtg gggcatcttc atcaggacct acttgagctg ggaggtcagc
      241 gtctccctct ccgtaggctt caagaccatg gacttccctg ccgtcaccat ctgcaatgct
      301 agccccttca agtattccaa aatcaagcat ttgctgaagg acctggatga gctgatggaa
      361 gctgtcctgg agagaatcct ggctcctgag ctaagccatg ccaatgccac caggaacctg
      421 aacttctcca tctggaacca cacacccctg gtccttattg atgaacggaa cccccaccac
      481 cccatggtcc ttgatctctt tggagacaac cacaatggct taacaagcag ctcagcatca
      541 gaaaagatct gtaatgccca cgggtgcaaa atggccatga gactatgtag cctcaacagg
      601 acccagtgta ccttccggaa cttcaccagt gctacccagg cattgacaga gtggtacatc
      661 ctgcaggcca ccaacatctt tgcacaggtg ccacagcagg agctagtaga gatgagctac
      721 cccggcgagc agatgatcct ggcctgccta ttcggagctg agccctgcaa ctaccggaac
      781 ttcacgtcca tcttctaccc tcactatggc aactgttaca tcttcaactg gggcatgaca
      841 gagaaggcac ttccttcggc caaccctgga actgaattcg gcctgaagtt gatcctggac
      901 ataggccagg aagactacgt ccccttcctt gcgtccacgg ccggggtcag gctgatgctt
      961 cacgagcaga ggtcataccc cttcatcaga gatgagggca tctacgccat gtcggggaca
     1021 gagacgtcca tcggggtact cgtggacaag cttcagcgca tgggggagcc ctacagcccg
     1081 tgcaccgtga atggttctga ggtccccgtc caaaacttct acagtgacta caacacgacc
     1141 tactccatcc aggcctgtct tcgctcctgc ttccaagacc acatgatccg taactgcaac
     1201 tgtggccact acctgtaccc actgccccgt ggggagaaat actgcaacaa ccgggacttc
     1261 ccagactggg cccattgcta ctcagatcta cagatgagcg tggcgcagag agagacctgc
     1321 attggcatgt gcaaggagtc ctgcaatgac acccagtaca agatgaccat ctccatggct
     1381 gactggcctt ctgaggcctc cgaggactgg attttccacg tcttgtctca ggagcgggac
     1441 caaagcacca atatcaccct gagcaggaag ggaattgtca agctcaacat ctacttccaa
     1501 gaatttaact atcgcaccat tgaagaatca gcagccaata acatcgtctg gctgctctcg
     1561 aatctgggtg gccagtttgg cttctggatg gggggctctg tgctgtgcct catcgagttt
     1621 ggggagatca tcatcgactt tgtgtggatc accatcatca agctggtggc cttggccaag
     1681 agcctacggc agcggcgagc ccaagccagc tacgctggcc caccgcccac cgtggccgag
     1741 ctggtggagg cccacaccaa ctttggcttc cagcctgaca cggccccccg cagccccaac
     1801 actgggccct accccagtga gcaggccctg cccatcccag gcaccccgcc ccccaactat
     1861 gactccctgc gtctgcagcc gctggacgtc atcgagtctg acagtgaggg tgatgccatc
     1921 tacccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc
     1981 gattctacgt ag
//
