LOCUS       sequence-31337-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RASL12A
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             634..651
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(704..722)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      718..765
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(889..908)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggcccc ctcgaggtca acctggccat
      121 cctggggcgc cgcggggctg gcaagtctgc cctgaccgtg aagtttctga ccaagaggtt
      181 tatcagtgaa tatgacccca acttggagga cacctacagc tccgaggaga ctgtggacca
      241 ccagcctgtc cacctgaggg tcatggacac tgcagacctg gacaccccca ggaactgcga
      301 gcgctacctg aactgggccc atgccttcct ggtggtgtac agcgtcgaca gccgccagag
      361 ctttgatagc agcagcagct acctggagct gcttgccttg cacgcgaagg agacacagcg
      421 cagcatccct gccctgctgc tgggcaacaa gctggacatg gctcagtaca ggcaagtcac
      481 caaggcagag ggtgtggctt tggcaggcag gtttgggtgc ctgtttttcg aggtctctgc
      541 ctgtctggac tttgagcacg tgcagcatgt cttccacgag gcagtgcgag aggcacggcg
      601 ggagtgatga cgaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      661 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      721 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      781 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      841 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      901 tcttcccttc ctttctcgcc acgttcgccg gc
//
