LOCUS       sequence-313521-        2874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid with single copy of Mma PylT (CUA anticodon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pUC_h7SK Mma PylT (CUA)
FEATURES             Location/Qualifiers
     source          1..2874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     426..445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     618..635
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(1213..1260)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(1313..1332)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1314..1332)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1343..1360)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1343..1359)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1352..1374)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1675..1779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1780..1848,1849..2640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1998..2017)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2811..2874,1..525)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
       61 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      121 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      181 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      241 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      301 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      361 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      421 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      481 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      541 gcctttttac ggttcctggc cttttgctgg ccttttgctc attaggcacc ccaggcttta
      601 cccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggagagcgcc caatacgcaa
      661 ggaaacagct atgaccatgt taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      721 gcagtgagcg gaaggcccat gaggccagtc ttgtgctcca ttaattaatc tcggcctctg
      781 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
      841 ggggcgggat agcactgact cctaggaaaa accgcacttg tccggaaacc ccgggaatct
      901 aacccggctg aacggattta gagtccgttc gatctacatg atcaggtttc cgaggtaccc
      961 aggcggcgca caagctatat aaacctgaag gaaatctcaa ctttacactt aggtcaagtt
     1021 acttatcgta ctagagcttc agcaggaaat ttaactaaaa tctaatttaa ccagcatagc
     1081 aaatatcatt tattcccaaa atgctaaagt ttgagataaa cggacttgat ttccggctgt
     1141 tttgacacta tccagaatgc cttgcagatg ggtggggcat gctaaatact gcagaactag
     1201 tgactgacta aggaagttcc tatactttct agagaatagg aacttcggaa taggaacttc
     1261 tagtggcgcg cctggacaag acacgtggcc ttgacggcct tccgccaatt cgccctatag
     1321 tgagtcgtat tacgtcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1381 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1441 gcgaagaggc ccgcaccgaa acgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     1501 gagcgccctg tagcggccac tcaaccctat ctcggtctat tcttttgatt tataagggat
     1561 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     1621 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     1681 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1741 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1801 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1861 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1921 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1981 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2041 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2101 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2161 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     2221 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     2281 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     2341 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2401 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2461 tttattgctg ataaatctgg agccggtgag cgtggttctc gcggtatcat tgcagcactg
     2521 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2581 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2641 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2701 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2761 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2821 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcg
//
