LOCUS       sequence-313612-        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a 
            C-terminal GFP and SNAP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Ferro LS, Fang Q, Eshun-Wilson L, Fernandes J, Jack A, Farrell DP, 
            Golcuk M, Huijben T, Costa K, Gur M, DiMaio F, Nogales E, Yildiz A
  TITLE     Structural and functional insight into regulation of kinesin-1 by 
            microtubule-associated protein MAP7.
  JOURNAL   Science. 2022 Jan 21;375(6578):326-331. doi: 
            10.1126/science.abf6154. Epub 2022 Jan 20.
  PUBMED    35050657
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abf6154"; journalName: "Science"; date: 
            "2022-01-21- 21"; volume: "375"; issue: "6578"; pages: "326-331"
COMMENT     Sequence Label: pOmnibac_ZZ_linker_K963_GFP_SNAP
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(132..137,138..154,155..160))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(231..264)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        341..432
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     357..374
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             499..522
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             565..738
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             739..912
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAEKLNDAQAPK"
     CDS             970..990
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1012..1032
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3928..4641
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3928..4641
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3958..3986)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4581..4601
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4648..5193
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHLVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     polyA_signal    5327..5461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5364..5383)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5418..5437
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5582..5747
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_recomb     5754..6459
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           5842..6214
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(5871..6459)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      6649..7237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7138..7157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_recomb     7493..8320
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             7493..7617
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(7655..8260)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     mobile_element  8349..8572
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8639..9172)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg
       61 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
      121 acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc
      181 gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgcc ataacttcgt
      241 atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag cgagtttaaa
      301 cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga taattaaaat
      361 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
      421 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctcggtcc
      481 gaaaccatgt cgtactacca tcaccatcac catcaccatc acggttctat gtgcatggca
      541 ggccttgcgc aacacgatga agccgtggac aacaaattca acaaagaaca acaaaacgcg
      601 tactatgaga tcttacattt acctaactta aacgaaggac aacgaaacgc cttcatccaa
      661 agtttaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa aaagctaaat
      721 gatgctcagg cgccgaaagt agacaacaaa ttcaacaaag aacaacaaaa cgcgttctat
      781 gagatcttac atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta
      841 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctgaaaagct aaatgatgct
      901 caggcgccga aagtagacgc aaattccgcg gggaagtcaa ccgattatga tattccaact
      961 actgctagcg agaacctata tttccaggga gattatgata tccctacgac tgagaatctt
     1021 tattttcagg gcatggcgga cctggccgag tgcaacatca aagtgatgtg tcgcttcaga
     1081 cctctcaacg agtctgaagt gaaccgcggc gacaagtaca tcgccaagtt tcagggagaa
     1141 gacacggtcg tgatcgcgtc caagccttat gcatttgatc gggtgttcca gtcaagcaca
     1201 tctcaagagc aagtgtataa tgactgtgca aagaagattg ttaaagatgt acttgaagga
     1261 tataatggaa caatatttgc atatggacaa acatcctctg ggaagacaca cacaatggag
     1321 ggtaaacttc atgatccaga aggcatggga attattccaa gaatagtgca agatattttt
     1381 aattatattt actccatgga tgaaaatttg gaatttcata ttaaggtttc atattttgaa
     1441 atatatttgg ataagataag ggacctgtta gatgtttcaa agaccaacct ttcagttcat
     1501 gaagacaaaa accgagttcc ctatgtaaag gggtgcacag agcgttttgt atgtagtcca
     1561 gatgaagtta tggataccat agatgaagga aaatccaaca gacatgtagc agttacaaat
     1621 atgaatgaac atagctctag gagtcacagt atatttctta ttaatgtcaa acaagagaac
     1681 acacaaacgg aacaaaagct gagtggaaaa ctttatctgg ttgatttagc tggtagtgaa
     1741 aaggttagta aaactggagc tgaaggtgct gtgctggatg aagctaaaaa catcaacaag
     1801 tcactttctg ctcttggaaa tgttatttct gctttggctg agggtagtac atatgttcca
     1861 tatcgagata gtaaaatgac aagaatcctt caagattcat taggtggcaa ctgtagaacc
     1921 actattgtaa tttgctgctc tccatcatca tacaatgagt ctgaaacaaa atctacactc
     1981 ttatttggcc aaagggccaa aacaattaag aacacagttt gtgtcaatgt ggagttaact
     2041 gcagaacagt ggaaaaagaa gtatgaaaaa gaaaaagaaa aaaataagat cctgcggaac
     2101 actattcagt ggcttgaaaa tgagctcaac agatggcgta atggggagac ggtgcctatt
     2161 gatgaacagt ttgacaaaga gaaagccaac ttggaagctt tcacagtgga taaagatatt
     2221 actcttacca atgataaacc agcaaccgca attggagtta taggaaattt tactgatgct
     2281 gaaagaagaa agtgtgaaga agaaattgct aaattataca aacagcttga tgacaaggat
     2341 gaagaaatta accagcaaag tcaactggta gagaaactga agacgcaaat gttggatcag
     2401 gaggagcttt tggcatctac cagaagggat caagacaata tgcaagctga gctgaatcgc
     2461 cttcaagcag aaaatgatgc ctctaaagaa gaagtgaaag aagttttaca ggccctagaa
     2521 gaacttgctg tcaattatga tcagaagtct caggaagttg aagacaaaac taaggaatat
     2581 gaattgctta gtgatgaatt gaatcagaaa tcggcaactt tagcgagtat agatgctgag
     2641 cttcagaaac ttaaggaaat gaccaaccac cagaaaaaac gagcagctga gatgatggca
     2701 tctttactaa aagaccttgc agaaatagga attgctgtgg gaaataatga tgtaaagcag
     2761 cctgagggaa ctggcatgat agatgaagag ttcactgttg caagactcta cattagcaaa
     2821 atgaagtcag aagtaaaaac catggtgaaa cgttgcaagc agttagaaag cacacaaact
     2881 gagagcaaca aaaaaatgga agaaaatgaa aaggagttag cagcatgtca gcttcgtatc
     2941 tctcaacatg aagccaaaat caagtcattg actgaatacc ttcaaaatgt ggaacaaaag
     3001 aaaagacagt tggaggaatc tgtcgatgcc ctcagtgaag aactagtcca gcttcgagca
     3061 caagagaaag tccatgaaat ggaaaaggag cacttaaata aggttcagac tgcaaatgaa
     3121 gttaagcaag ctgttgaaca gcagatccag agccatagag aaactcatca aaaacagatc
     3181 agtagtttga gagatgaagt agaagcaaaa gcaaaactta ttactgatct tcaagaccaa
     3241 aaccagaaaa tgatgttaga gcaggaacgt ctaagagtag aacatgagaa gttgaaagcc
     3301 acagatcagg aaaagagcag aaaactacat gaacttacgg ttatgcaaga tagacgagaa
     3361 caagcaagac aagacttgaa gggtttggaa gagacagtgg caaaagaact tcagacttta
     3421 cacaacctgc gcaaactctt tgttcaggac ctggctacaa gagttaaaaa gagtgctgag
     3481 attgattctg atgacaccgg aggcagcgct gctcagaagc aaaaaatctc ctttcttgaa
     3541 aataatcttg aacagctcac taaagtgcac aaacagttgg tacgtgataa tgcagatctc
     3601 cgctgtgaac ttcctaagtt ggaaaagcga cttcgagcta cagctgagag agtgaaagct
     3661 ttggaatcag cactgaaaga agctaaagaa aatgcatctc gtgatcgcaa acgctatcag
     3721 caagaagtag atcgcataaa ggaagcagtc aggtcaaaga atatggccag aagagggcat
     3781 tctgcacaga ttgctaaacc tattcgtccc gggcaacatc cagcagcttc tccaactcac
     3841 ccaagtgcaa ttcgtggagg aggtgcattt gttcagaaca gccagccagt ggcagtgcga
     3901 ggtggaggag gcaaacaagt gggttctatg agtaaaggag aagaactttt cactggagtt
     3961 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     4021 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     4081 aaactacctg ttccatggcc aacacttgtc actactctga cttatggtgt tcaatgcttt
     4141 tcaagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
     4201 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     4261 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     4321 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     4381 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     4441 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     4501 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     4561 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     4621 ggcatggatg aactatacaa aggttctatg gacaaagact gcgaaatgaa gcgcaccacc
     4681 ctggatagcc ctctgggcaa gctggaactg tctgggtgcg aacagggcct gcaccgtatc
     4741 atcttcctgg gcaaaggaac atctgccgcc gacgccgtgg aagtgcctgc cccagccgcc
     4801 gtgctgggcg gaccagagcc actgatgcag gccaccgcct ggctcaacgc ctactttcac
     4861 cagcctgagg ccatcgagga gttccctgtg ccagccctgc accacccagt gttccagcag
     4921 gagagcttta cccgccaggt gctgtggaaa ctgctgaaag tggtgaagtt cggagaggtc
     4981 atcagctaca gccacctggc cgccctggcc ggcaatcccg ccgccaccgc cgccgtgaaa
     5041 accgccctga gcggaaatcc cgtgcccatt ctgatcccct gccacctggt ggtgcagggc
     5101 gacctggacg tggggggcta cgagggcggg ctcgccgtga aagagtggct gctggcccac
     5161 gagggccaca gactgggcaa gcctgggctg ggttaatgag cttgtcgaga agtactagag
     5221 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     5281 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     5341 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5401 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     5461 ctgatcactg cttgagccta gaagatccgg ctgctaacaa agcccgaaag gaagctgagt
     5521 tggctgctgc caccgctgag caataactat cataacccct agggtatacc catctaattg
     5581 gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc
     5641 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa
     5701 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaacc ggtggaggaa
     5761 attctccttg aagtttccct ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg
     5821 ttcactggtc cggcgtatta aaacacgata cattgttatt agtacattta ttaagcgcta
     5881 gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa ttcattaaat
     5941 ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc gtctttatat
     6001 ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac
     6061 aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct caacgccaca
     6121 aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg tgttttgttt
     6181 tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct ttcatcactg
     6241 tcgttagtgt acaattgact cgacgtaaac acgttaaata gagcttggac atatttaaca
     6301 tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa
     6361 gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt gtcggctaac
     6421 acgtccgcga tcaaatttgt agttgagctt tttggaatta ccggttgact tgggtcaact
     6481 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6541 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6601 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6661 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6721 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6781 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6841 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6901 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6961 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7021 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7081 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7141 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7201 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7261 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7321 ttgacttggg tcgctcttcc tgtggatgcg caggtatgta caggaagagg tttatactaa
     7381 actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg
     7441 ctctgacgca tttctacaac cacgactcta agtgtgtggg tgaagtcatg catgcatctt
     7501 ttaatcaaat cccaagatgt gtataaacca ccaaactgcc aaaaaatgaa aactgtcgac
     7561 aagctctgtc cgtttgctgg caactgcaag ggtctcaatc ctatttgtaa ttattgaata
     7621 ataaaacaat tataaatgtc aaatttgttt tttattaacg atacaaacca aacgcaacaa
     7681 gaacatttgt agtattatct ataattgaaa acgcgtagtt ataatcgctg aggtaatatt
     7741 taaaatcatt ttcaaatgat tcacagttaa tttgcgacaa tataatttta ttttcacata
     7801 aactagacgc cttgtcgtct tcttcttcgt attccttctc tttttcattt ttctcttcat
     7861 aaaaattaac atagttatta tcgtatccat atatgtatct atcgtataga gtaaattttt
     7921 tgttgtcata aatatatatg tcttttttaa tggggtgtat agtaccgctg cgcatagttt
     7981 ttctgtaatt tacaacagtg ctattttctg gtagttcttc ggagtgtgtt gctttaatta
     8041 ttaaatttat ataatcaatg aatttgggat cgtcggtttt gtacaatatg ttgccggcat
     8101 agtacgcagc ttcttctagt tcaattacac cattttttag cagcaccgga ttaacataac
     8161 tttccaaaat gttgtacgaa ccgttaaaca aaaacagttc acctcccttt tctatactat
     8221 tgtctgcgag cagttgtttg ttgttaaaaa taacagccat tgtaatgaga cgcacaaact
     8281 aatatcacaa actggaaatg tctatcaata tatagttgct gattgcgcag atgccctgcg
     8341 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
     8401 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
     8461 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
     8521 tgcccgtcgt attaaagagg ggcgtggcca agggcatgta aagactatat tcgcggcgtt
     8581 gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga acgaattgtt
     8641 aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg
     8701 tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac
     8761 caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc
     8821 ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact
     8881 tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg
     8941 cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg
     9001 agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt
     9061 tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact
     9121 ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg
     9181 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgg
//
