LOCUS       sequence-313641-       10500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lenti-viral construct with Cre recombinase and doxycycline inducible
            expression of mouse Notch intracellular domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Loganathan SK, Schleicher K, Malik A, Quevedo R, Langille E, Teng K,
            Oh RH, Rathod B, Tsai R, Samavarchi-Tehrani P, Pugh TJ, Gingras AC, 
            Schramek D
  TITLE     Rare driver mutations in head and neck squamous cell carcinomas 
            converge on NOTCH signaling.
  JOURNAL   Science. 2020 Mar 13;367(6483):1264-1269. doi: 
            10.1126/science.aax0902.
  PUBMED    32165588
REFERENCE   2  (bases 1 to 10500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-03-13- 13"; volume: "367"; 
            issue: "6483"; pages: "1264-1269"
COMMENT     Sequence Label: PLX306 iCre tetO NICD rtTA
FEATURES             Location/Qualifiers
     source          1..10500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2696..2737
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2751..2768
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2778..3288
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3207..3225
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4345..4401
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4402..5019,5020..5025,5026..5061,5062..5064,5065..5100,
                     5101..5103,5104..5139,5140..5148)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5188..5208)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5606..5617)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5822..6055
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6127..6248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6164..6183)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6218..6237
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6288..6423
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6423..6494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6495..6563,6564..7355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6713..6732)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7526..8114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8015..8034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8268..8285
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8402..8423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8438..8443,8444..8461,8462..8468)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8476..8492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8481..8503
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8500..8516
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8500..8516
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8535..8555
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8537..8555
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8583..8809
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8810..8990
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9037..9162
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9655..9888
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10073..10117
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10330..10447
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag aattggctag cgtgctgctg tcccgcaagc gccggcggca
      361 gcatggccag ctctggttcc ctgagggttt caaagtgtca gaggccagca agaagaagcg
      421 gagagagccc ctcggcgagg actcagtcgg cctcaagccc ctgaagaatg cctcagatgg
      481 tgctctgatg gacgacaatc agaacgagtg gggagacgaa gacctggaga ccaagaagtt
      541 ccggtttgag gagccagtag ttctccctga cctgagtgat cagactgacc acagacagtg
      601 gacccagcag cacctggacg ctgctgacct gcgcatgtct gccatggccc caacaccgcc
      661 tcagggggag gtggatgctg actgcatgga tgtcaatgtt cgaggaccag atggcttcac
      721 acccctcatg attgcctcct gcagtggagg gggccttgag acaggcaaca gtgaagaaga
      781 agaagatgca cctgctgtca tctctgactt catctaccag ggcgccagct tgcacaacca
      841 gacagaccgc accggggaga ccgccttgca cttggctgcc cgatactctc gttcagatgc
      901 tgcaaagcgc ttgctggagg ccagtgcaga tgccaacatc caggacaaca tgggccgtac
      961 tccgttacat gcagcagttt ctgcagatgc tcagggtgtc ttccagatcc tgctccggaa
     1021 cagggccaca gatctggatg cccgaatgca tgatggcaca actccactga tcctggctgc
     1081 gcgcctggcc ctggagggca tgctggagga cctcatcaac tcacatgctg acgtcaatgc
     1141 cgtggatgac ctaggcaagt cggctttgca ttgggcggcc gcggtgaaca atgtggatgc
     1201 tgctgttgtg ctcctgaaga acggagccaa caaggacatg cagaacaaca aggaggagac
     1261 tcccctgttc ctggccgccc gtgagggcag ctatgagact gccaaagtgt tgctggacca
     1321 ctttgccaac cgggacatca cggatcacat ggaccgattg ccgcgggaca tcgcacagga
     1381 gcgtatgcac cacgatatcg tgcggctttt ggatgagtac aacctggtgc gcagcccaca
     1441 gctgcatggc actgccctgg gtggcacacc cactctgtct cccacactct gctcgcccaa
     1501 tggctacctg ggcaatctca agtctgccac acagggcaag aaggcccgca agcccagcac
     1561 caaagggctg gcttgtggta gcaaggaagc taaggacctc aaggcacgga ggaagaagtc
     1621 ccaggatggc aagggctgcc tgttggacag ctcgagcatg ctgtcgcctg tggactccct
     1681 cgagtcaccc catggctact tgtcagatgt ggcctcgcca cccctcctcc cctccccatt
     1741 ccagcagtct ccatccatgc ctctcagcca cctgcctggt atgcctgaca ctcacctggg
     1801 catcagccac ttgaatgtgg cagccaagcc tgagatggca gcactggctg gaggtagccg
     1861 gttggccttt gagccacccc cgccacgcct ctcccacctg cctgtagcct ccagtgccag
     1921 cacagtgctg agtaccaatg gcacgggggc tatgaatttc accgtgggtg caccggcaag
     1981 cttgaatggc cagtgtgagt ggcttccccg gctccagaat ggcatggtgc ccagccagta
     2041 caacccacta cggccgggtg tgacgccggg cacactgagc acacaggcag ctggcctcca
     2101 gcatagcatg atggggccac tacacagcag cctctccacc aataccttgt ccccgattat
     2161 ttaccagggc ctgcccaaca cacggctggc aacacagcct cacctggtgc agacccagca
     2221 ggtgcagcca cagaacttac agctccagcc tcagaacctg cagccaccat cacagccaca
     2281 cctcagtgtg agctcggcag ccaatgggca cctgggccgg agcttcttga gtggggagcc
     2341 cagtcaggca gatgtacaac cgctgggccc cagcagtctg cctgtgcaca ccattctgcc
     2401 ccaggaaagc caggccctgc ccacatcact gccatcctcc atggtcccac ccatgaccac
     2461 tacccagttc ctgacccctc cttcccagca cagttactcc tcctcccctg tggacaacac
     2521 ccccagccac cagctgcagg tgccagagca ccccttcctc accccatccc ctgagtcccc
     2581 tgaccagtgg tccagctcct ccccgcattc caacatctct gattggtccg agggcatctc
     2641 cagcccgccc accaccatgc cgtcccagat cacccacatt ccagaggcat ttaaaggaaa
     2701 gccaatccca aacccactcc taggtctcga cagtacagca tagaccggtc caccaccacc
     2761 accaccacta aggatccggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg
     2821 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     2881 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     2941 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     3001 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     3061 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     3121 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     3181 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     3241 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg tttaaacatg
     3301 gtctccaacc tgctgactgt gcaccaaaac ctgcctgccc tccctgtgga tgccacctct
     3361 gatgaagtca ggaagaacct gatggacatg ttcagggaca ggcaggcctt ctctgaacac
     3421 acctggaaga tgctcctgtc tgtgtgcaga tcctgggctg cctggtgcaa gctgaacaac
     3481 aggaaatggt tccctgctga acctgaggat gtgagggact acctcctgta cctgcaagcc
     3541 agaggcctgg ctgtgaagac catccaacag cacctgggcc agctcaacat gctgcacagg
     3601 agatctggcc tgcctcgccc ttctgactcc aatgctgtgt ccctggtgat gaggagaatc
     3661 agaaaggaga atgtggatgc tggggagaga gccaagcagg ccctggcctt tgaacgcact
     3721 gactttgacc aagtcagatc cctgatggag aactctgaca gatgccagga catcaggaac
     3781 ctggccttcc tgggcattgc ctacaacacc ctgctgcgca ttgccgaaat tgccagaatc
     3841 agagtgaagg acatctcccg caccgatggt gggagaatgc tgatccacat tggcaggacc
     3901 aagaccctgg tgtccacagc tggtgtggag aaggccctgt ccctgggggt taccaagctg
     3961 gtggagagat ggatctctgt gtctggtgtg gctgatgacc ccaacaacta cctgttctgc
     4021 cgggtcagaa agaatggtgt ggctgcccct tctgccacct cccaactgtc cacccgggcc
     4081 ctggaaggga tctttgaggc cacccaccgc ctgatctatg gtgccaagga tgactctggg
     4141 cagagatacc tggcctggtc tggccactct gccagagtgg gtgctgccag ggacatggcc
     4201 agggctggtg tgtccatccc tgaaatcatg caggctggtg gctggaccaa tgtgaacatt
     4261 gtgatgaact acatcagaaa cctggactct gagactgggg ccatggtgag gcttctagag
     4321 gatggggatg gctccggaga attcgccacg aacttctctc tgttaaagca agcaggagac
     4381 gtggaagaaa accccggtcc catgtctaga ctggacaaga gcaaagtcat aaacggagct
     4441 ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     4501 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     4561 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     4621 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     4681 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     4741 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     4801 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     4861 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     4921 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     4981 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac
     5041 gattttgact tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg
     5101 cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat
     5161 cgatccaaga tatcgtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5221 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5281 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5341 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5401 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5461 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5521 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     5581 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5641 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5701 ctccctttgg gccgcctccc cgcctgtttc gcctcggcgt ccggactaga ggtaccttta
     5761 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5821 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5881 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5941 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6001 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     6061 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6121 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6241 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6301 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6361 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6421 gcctttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6481 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6541 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6601 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6661 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6721 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6781 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6841 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6901 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6961 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7021 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7081 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7141 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7201 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7261 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7321 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7381 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7441 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7501 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7561 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7621 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7681 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7741 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7801 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7861 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7921 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7981 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8041 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8101 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8161 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8221 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8281 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8341 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8401 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8461 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     8521 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     8581 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     8641 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     8701 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     8761 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     8821 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8881 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8941 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     9001 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     9061 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     9121 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     9181 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     9241 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     9301 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     9361 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     9421 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     9481 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     9541 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     9601 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     9661 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     9721 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     9781 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     9841 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     9901 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     9961 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    10021 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    10081 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    10141 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    10201 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    10261 tcagacccac ctcccaaccc cgaggggaca attctcgacc tcgagacaaa tggcagtatt
    10321 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
    10381 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    10441 aaattttcgg gtttattaca gggacagcag agatccactt tggccgcgaa tcgatatgtc
//
