LOCUS       sequence-313648-       10220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Yanagiya A, Svitkin YV, Shibata S, Mikami S, Imataka H, Sonenberg N
  TITLE     Requirement of RNA binding of mammalian eukaryotic translation 
            initiation factor 4GI (eIF4GI) for efficient interaction of eIF4E 
            with the mRNA cap.
  JOURNAL   Mol Cell Biol. 2009 Mar;29(6):1661-9. doi: 10.1128/MCB.01187-08. 
            Epub 2008 Dec 29.
  PUBMED    19114555
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01187-08"; journalName: "Mol Cell Biol"; date: 
            "2009-03"; volume: "29"; issue: "6"; pages: "1661-9"
COMMENT     Sequence Label: pcDNA3 HA eIF4GI (1-1599)
FEATURES             Location/Qualifiers
     source          1..10220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      661..670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             673..699
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(5539..5557)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5540..5557)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5577..5594)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5583..5807
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5853..6281
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5940..5959)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6150..6171
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6290..6310)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6295..6624
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6475..6610
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6537..6556
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6691..7485
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6745..6764)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7355..7374
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7659..7780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7696..7715)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7750..7769
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7829..7845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7829..7845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7842..7864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7853..7869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7877..7883,7884..7901,7902..7907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7922..7943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8060..8077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8231..8819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8311..8330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8990..9781,9782..9850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9613..9632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9851..9955)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10030..10049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 gccaccatgg gatatccata cgacgtccca gactatgctg gaatgaacaa agctccacag
      721 tccacaggcc ccccacccgc cccatccccc ggactcccac agccagcgtt tcccccgggg
      781 cagacagcgc cggtggtgtt cagtacgcca caagcgacac aaatgaacac gccttctcag
      841 ccccgccagc acttctaccc tagccgggcc cagcccccga gcagtgcagc ctcccgagtg
      901 cagagtgcag cccctgcccg ccctggccca gctgcccatg tctaccctgc tggatcccaa
      961 gtaatgatga tcccttccca gatctcctac ccagcctccc agggggccta ctacatccct
     1021 ggacaggggc gttccacata cgttgtcccg acacagcagt accctgtgca gccaggagcc
     1081 ccaggcttct atccaggtgc aagccctaca gaatttggga cctacgctgg cgcctactat
     1141 ccagcccaag gggtgcagca gtttcccact ggcgtggccc ccgccccagt tttgatgaac
     1201 cagccacccc agattgctcc caagagggag cgtaagacga tccgaattcg agatccaaac
     1261 caaggaggaa aggatatcac agaggagatc atgtctgggg cccgcactgc ctccacaccc
     1321 acccctcccc agacgggagg cggtctggag cctcaagcta atggggagac gccccaggtt
     1381 gctgtcattg tccggccaga tgaccggtca cagggagcaa tcattgctga ccggccaggg
     1441 ctgcctggcc cagagcatag cccttcagaa tcccagcctt cgtcgccttc tccgacccca
     1501 tcaccatccc cagtcttgga accggggtct gagcctaatc tcgcagtcct ctctattcct
     1561 ggggacacta tgacaactat acaaatgtct gtagaagaat caacccccat ctcccgtgaa
     1621 actggggagc catatcgcct ctctccagaa cccactcctc tcgccgaacc catactggaa
     1681 gtagaagtga cacttagcaa accggttcca gaatctgagt tttcttccag tcctctccag
     1741 gctcccaccc ctttggcatc tcacacagtg gaaattcatg agcctaatgg catggtccca
     1801 tctgaagatc tggaaccaga ggtggagtca agcccagagc ttgctcctcc cccagcttgc
     1861 ccctccgaat cccctgtgcc cattgctcca actgcccaac ctgaggaact gctcaacgga
     1921 gccccctcgc caccagctgt ggacttaagc ccagtcagtg agccagagga gcaggccaag
     1981 gaggtgacag catcagtggc gccccccacc atcccctctg ctactccagc tacggctcct
     2041 tcagctactt ccccagctca ggaggaggaa atggaagaag aagaagaaga ggaagaagga
     2101 gaagcaggag aagcaggaga agctgagagt gagaaaggag gagaggaact gctcccccca
     2161 gagagtaccc ctattccagc caacttgtct cagaatttgg aggcagcagc agccactcaa
     2221 gtggcagtat ctgtgccaaa gaggagacgg aaaattaagg agctaaataa gaaggaggct
     2281 gttggagacc ttctggatgc cttcaaggag gcgaacccgg cagtaccaga ggtggaaaat
     2341 cagcctcctg caggcagcaa tccaggccca gagtctgagg gcagtggtgt gcccccacgt
     2401 cctgaggaag cagatgagac ctgggactca aaggaagaca aaattcacaa tgctgagaac
     2461 atccagcccg gggaacagaa gtatgaatat aagtcagatc agtggaagcc tctaaaccta
     2521 gaggagaaaa aacgttacga ccgtgagttc ctgcttggtt ttcagttcat ctttgccagt
     2581 atgcagaagc cagagggatt gccacatatc agtgacgtgg tgctggacaa ggccaataaa
     2641 acaccactgc ggccactgga tcccactaga ctacaaggca taaattgtgg cccagacttc
     2701 actccatcct ttgccaacct tggccggaca acccttagca cccgtgggcc cccaaggggt
     2761 gggccaggtg gggagctgcc ccgtgggccg gctggcctgg gaccccggcg ctctcagcag
     2821 ggaccccgaa aagaaccacg caagatcatt gccacagtgt taatgaccga agatataaaa
     2881 ctgaacaaag cagagaaagc ctggaaaccc agcagcaagc ggacggcggc tgataaggat
     2941 cgaggggaag aagatgctga tggcagcaaa acccaggacc tattccgcag ggtgcgctcc
     3001 atcctgaata aactgacacc ccagatgttc cagcagctga tgaagcaagt gacgcagctg
     3061 gccatcgaca ccgaggaacg cctcaaaggg gtcattgacc tcatttttga gaaggccatt
     3121 tcagagccca acttctctgt ggcctatgcc aacatgtgcc gctgcctcat ggcgctgaaa
     3181 gtgcccacta cggaaaagcc aacagtgact gtgaacttcc gaaagctgtt gttgaatcga
     3241 tgtcagaagg agtttgagaa agacaaagat gatgatgagg tttttgagaa gaagcaaaaa
     3301 gagatggatg aagctgctac ggcagaggaa cgaggacgcc tgaaggaaga gctggaagag
     3361 gctcgggaca tagcccggcg gcgctcttta gggaatatca agtttattgg agagttgttc
     3421 aaactgaaga tgttaacaga ggcaataatg catgactgtg tggtcaaact gcttaagaac
     3481 catgatgaag agtcccttga gtgcctttgt cgtctgctca ccaccattgg caaagacctg
     3541 gactttgaaa aagccaagcc ccgaatggat cagtatttca accagatgga aaaaatcatt
     3601 aaagaaaaga agacgtcatc ccgcatccgc tttatgctgc aggacgtgct ggatctgcga
     3661 gggagcaatt gggtgccacg ccgaggggat cagggtccca agaccattga ccagatccat
     3721 aaggaggctg agatggaaga acatcgagag cacatcaaag tgcagcagct catggccaag
     3781 ggcagtgaca agcgtcgggg cggtcctcca ggccctccca tcagccgtgg acttcccctt
     3841 gtggatgatg gtggctggaa cacagttccc atcagcaaag gtagccgccc cattgacacc
     3901 tcacgactca ccaagatcac caagcctggc tccatcgatt ctaacaacca gctctttgca
     3961 cctggagggc gactgagctg gggcaagggc agcagcggag gctcaggagc caagccctca
     4021 gacgcagcat cagaagctgc tcgcccagct actagtactt tgaatcgctt ctcagccctt
     4081 caacaagcgg tacccacaga aagcacagat aatagacgtg tggtgcagag gagtagcttg
     4141 agccgagaac gaggcgagaa agctggagac cgaggagacc gcctagagcg gagtgaacgg
     4201 ggaggggacc gtggggaccg gcttgatcgt gcgcggacac ctgctaccaa gcggagcttc
     4261 agcaaggaag tggaggagcg gagtagagaa cggccctccc agcctgaggg gctgcgcaag
     4321 gcagctagcc tcacggagga tcgggaccgt gggcgggatg ccgtgaagcg agaagctgcc
     4381 ctacccccag tgagccccct gaaggcggct ctctctgagg aggagttaga gaagaaatcc
     4441 aaggctatca ttgaggaata tctccatctc aatgacatga aagaggcagt ccagtgcgtg
     4501 caggagctgg cctcaccctc cttgctcttc atctttgtac ggcatggtgt cgagtctacg
     4561 ctggagcgca gtgccattgc tcgtgagcat atggggcagc tgctgcacca gctgctctgt
     4621 gctgggcatc tgtctactgc tcagtactac caagggttgt atgaaatctt ggaattggct
     4681 gaggacatgg aaattgacat cccccacgtg tggctctacc tagcggaact ggtaacaccc
     4741 attctgcagg aaggtggggt gcccatgggg gagctgttca gggagattac aaagcctctg
     4801 agaccgttgg gcaaagctgc ttccctgttg ctggagatcc tgggcctcct gtgcaaaagc
     4861 atgggtccta aaaaggtggg gacgctgtgg cgagaagccg ggcttagctg gaaggaattt
     4921 ctacctgaag gccaggacat tggtgcattc gtcgctgaac agaaggtgga gtataccctg
     4981 ggagaggagt cggaagcccc tggccagagg gcactcccct ccgaggagct gaacaggcag
     5041 ctggagaagc tgctgaagga gggcagcagt aaccagcggg tgttcgactg gatagaggcc
     5101 aacctgagtg agcagcagat agtatccaac acgttagttc gagccctcat gacggctgtc
     5161 tgctattctg caattatttt tgagactccc ctccgagtgg acgttgcagt gctgaaagcg
     5221 cgagcgaagc tgctgcagaa atacctgtgt gacgagcaga aggagctaca ggcgctctac
     5281 gccctccagg cccttgtagt gaccttagaa cagcctccca acctgctgcg gatgttcttt
     5341 gacgcactgt atgacgagga cgtggtgaag gaggatgcct tctacagttg ggagagtagc
     5401 aaggaccccg ctgagcagca gggcaagggt gtggccctta aatctgtcac agccttcttc
     5461 aagtggctcc gtgaagcaga ggaggagtct gaccacaact gataaataaa actcgagcat
     5521 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
     5581 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5641 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5701 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5761 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5821 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5881 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5941 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6001 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6061 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6121 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6181 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6241 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6301 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6361 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6421 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6481 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6541 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6601 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6661 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6721 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6781 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6841 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6901 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6961 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7021 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7081 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7141 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7201 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7261 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7321 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7381 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7441 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     7501 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     7561 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7621 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7681 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7741 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7801 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7861 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7921 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7981 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8041 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8221 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8401 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8521 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8641 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8821 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8881 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8941 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9001 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9061 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9121 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9181 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9241 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9301 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9361 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9421 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9481 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9541 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9601 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9661 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9721 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9781 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9841 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9901 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9961 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
    10021 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
    10081 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
    10141 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
    10201 acgggccaga tatacgcgtt
//
