LOCUS       sequence-313803-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AGSC domain from Pontibacillus A/G cyclase enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  TITLE     Bacterial expression plasmids for production of recombinant AGSC 
            domains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX4-4T-1 Pontibacillus AGSC
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             460..477
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(540..562)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(707..725)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        793..896
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcggatctgg ttccgcgtgg atccgaaaac ctctacttcc aaggttttga ctgggagaag
       61 tacttgaata gtttctcatt tctcccaaag accaaagatt tcaacaatta tataagcccg
      121 acagactttg aagtggtctg ttatgtcaaa aagaacgact cagaccaata cactcggtac
      181 ttccagtcct cttccagtct tgataagggc atgaaggtta tgtttgagct tttcaattat
      241 gccaatatgc cgattggcaa cataaaatgg cgcgtcgtta attatggcga tgaggccgag
      301 caatctgaga aggacctcga atactatatg aaagattttg aaggcgagta cttctgtgag
      361 caaagtaccg catacacagg gcttcatttt atggaatgct acatctataa tccggaatcc
      421 aagctgatcg caaaagatag gtcagggatc tacattaatc atcatcatca ccatcattag
      481 cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac
      541 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
      601 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
      661 cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc
      721 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      841 ccgctcatga gacaataacc ctgataatgc ttcaataata ttgaaaaagg aagagtatga
      901 gtattcacat ttccgtgtcg cccttattcc ctttttgcng cattttgcnn ctgttttgct
      961 cncccanaaa cgctggtgaa gtaaagatgc tganatcann nggtgcacna nnngggttac
     1021 atcgaactgg ntnntcanc
//
