LOCUS       sequence-313852-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3529
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(520..544)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             552..593
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cctctgagga ccagatcgta ctgctgaagt caagtgccat tgaggtcatc atgttgcgct
       61 ccaatgagtc cttcaccatg gacgacatgt cctggacctg tggcaaccaa gactacaagt
      121 accgcgtcag tgacgtgacc aaagccggac acagcctgga gctgattgag cccctcatca
      181 agttccaggt gggactgaag aagctgaact tgcatgagga ggagcatgtc ctgctcatgg
      241 ccatctgcat cgtctcccca gatcgtcctg gggtgcagga cgccgcgctg attgaggcca
      301 tccaggaccg cctgtccaac acactgcaga cgtacatccg ctgccgccac ccgcccccgg
      361 gcagccacct gctctatgcc aagatgatcc agaagctagc cgacctgcgc agcctcaatg
      421 aggagcactc caagcagtac cgctgcctct ccttccagcc tgagtgcagc atgaagctaa
      481 cgccccttgt gctcgaagtg tttggcaatg agatctcctg cccaactttc ttgtacaaag
      541 tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
      601 gaactagccc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
      661 acgagtttct tcaagctgcg cttccaatac gcgttaagtc gacaatcaac ctctggatta
      721 caaaa
//
