LOCUS       sequence-313874-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3531
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(437..461)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          490..693
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(513..533)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtgcat agccggccgc tacttgtatc
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tgggcagttt tctgtgcctt cctttccagc ttcctgcttt
      241 tcctccttct tccctttagc acctctgcta atctttgctc caggttcttt cttagcagat
      301 gtttttcttg gtttgggttc aggttttggt ggagcaggtt tcgctgacaa tctggcagac
      361 cgtcttgtgg gctcctgttt agttactttg gatccatctt tgccctctgt attctctgga
      421 gactttctct tcggcatgcc aacttttttg tacaaacttg ttgatcaatc gatgctagcc
      481 tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa
      541 ccaagatcca aactcaaaaa gggcaaattc caaggagaat tacatcaagt gccaagctgg
      601 cctaacttca gtctccaccc actcagtgtg gggaaactcc atcgcataaa acccctcccc
      661 ccaacctaaa gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc
      721 ccggtactcc gtgg
//
