LOCUS       sequence-313929-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP37
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      732..741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             741..764
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             777..803
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    819..843
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3784..3808)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3827..4314
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3862..3885)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4247..4266
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4335..4934
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4335..4354)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4831..4851
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    5520..5536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5544..5550,5551..5568,5569..5574))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5589..5610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5727..5744)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5898..6486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5978..5997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6657..7448,7449..7517))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7280..7299
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7518..7622)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7746..7768)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7868..7887
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8081..8103
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8231..8250
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             8450..8966
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    9030..9371
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             9438..9854
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9784..9806
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9822..9838
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggggatc tgccaccatg gactacaagg atgacgatga caagctcgat ggaggatacc
      781 catacgatgt tccagattac gctgctcgag gttatcaaac aagtttgtac aaaaaagttg
      841 gcatgtctcc tctgaagata catggtccta tcagaattcg aagtatgcag actgggatta
      901 caaagtggaa agaaggatcc tttgaaattg tagaaaaaga gaataaagtc agcctagtag
      961 ttcactacaa tactggagga attccaagga tatttcagct aagtcataac attaaaaatg
     1021 tggtgcttcg acccagtgga gcgaaacaaa gccgcctaat gttaactctg caagataaca
     1081 gcttcttgtc tattgacaaa gtaccaagta aggatgcaga ggaaatgagg ttgtttctag
     1141 atgcagtcca tcaaaacaga cttcctgcag ccatgaaacc gtctcagggg tctggtagtt
     1201 ttggagccat tctgggcagc aggacctcac agaaggaaac cagcaggcag ctttcttact
     1261 cagacaatca ggcttctgca aaaagaggaa gtttggaaac taaagatgat attccatttc
     1321 gaaaagttct tggtaatccg ggtagaggat cgattaagac tgtagcagga agtggaatag
     1381 ctcggacgat tccttctttg acatctactt caacacctct tagatcaggg ttgctagaaa
     1441 atcgtactga aaagaggaaa agaatgatat caactggctc agaattgaat gaagattacc
     1501 ctaaggaaaa tgattcatca tcgaacaaca aggccatgac agatccctcc agaaagtatt
     1561 taaccagcag tagagaaaag cagctgagtt tgaaacagtc agaagagaat aggacatcag
     1621 ggcttttacc tttacagtca tcatcctttt atggtagcag agctggatcc aaggaacact
     1681 cttctggtgg cactaactta gacaggacta atgtttcaag ccagactccc tctgccaaaa
     1741 gaagtttggg atttcttcct cagccagttc ctctttctgt taaaaaactg aggtgtaacc
     1801 aggattacac tggctggaat aaaccaagag tgcccctttc ctctcaccaa cagcagcaac
     1861 tgcagggctt ctccaatttg ggaaatacct gctatatgaa tgctattcta caatctctat
     1921 tttcactcca gtcatttgca aatgacttgc ttaaacaagg tatcccatgg aagaaaattc
     1981 cactcaatgc acttatcaga cgctttgcac acttgcttgt taaaaaagat atctgtaatt
     2041 cagagaccaa aaaggattta ctcaagaagg ttaaaaatgc catttcagct acagcagaga
     2101 gattctctgg ttatatgcag aatgatgctc atgaattttt aagtcagtgt ttggaccagc
     2161 tgaaagaaga tatggaaaaa ttaaataaaa cttggaagac tgaacctgtt tctggagaag
     2221 aaaattcacc agatatttca gctaccagag catacacttg ccctgttatt actaatttgg
     2281 agtttgaggt tcagcactcc atcatttgta aagcatgtgg agagattatc cccaaaagag
     2341 aacagtttaa tgacctctct attgaccttc ctcgtaggaa aaaaccactc cctcctcgtt
     2401 caattcaaga ttctcttgat cttttcttta gggccgaaga actggagtat tcttgtgaga
     2461 agtgtggtgg gaagtgtgct cttgtcaggc acaaatttaa caggcttcct agggtcctca
     2521 ttctccattt gaaacgatat agcttcaatg tggctctctc gcttaacaat aagattgggc
     2581 agcaagtcat cattccaaga tacctgaccc tgtcatctca ttgcactgaa aatacaaaac
     2641 caccttttac ccttggttgg agtgcacata tggcaatttc tagaccattg aaagcctctc
     2701 aaatggtgaa ttcctgcatc accagccctt ctacaccttc aaagaaattc accttcaaat
     2761 ccaagagctc cttggcttta tgccttgatt cagacagtga ggatgagcta aaacgttctg
     2821 tggccctcag ccagagactt tgtgaaatgt taggcaacga acagcagcag gaagacctgg
     2881 aaaaagattc aaaattatgc ccaatagagc ctgacaagtc tgaattggaa aactcaggat
     2941 ttgacagaat gagcgaagaa gagcttctag cagctgtctt ggagataagt aagagagatg
     3001 cttcaccatc tctgagtcat gaagatgatg ataagccaac tagcagccca gataccggat
     3061 ttgcagaaga tgatattcaa gaaatgccag aaaatccaga cactatggaa actgagaagc
     3121 ccaaaacaat cacagagctg gatcctgcca gttttactga gataactaaa gactgtgatg
     3181 agaataaaga aaacaaaact ccagaaggat ctcagggaga agttgattgg ctccagcagt
     3241 atgatatgga gcgtgaaagg gaagagcaag agcttcagca ggcactggct cagagccttc
     3301 aagagcaaga ggcttgggaa cagaaagaag atgatgacct caaaagagct accgagttaa
     3361 gtcttcaaga gtttaacaac tcctttgtgg atgcattggg ttctgatgag gactctggaa
     3421 atgaggatgt ttttgatatg gagtacacag aagctgaagc tgaggaactg aaaagaaatg
     3481 ctgagacagg aaatctgcct cattcgtacc ggctcatcag tgttgtcagt cacattggta
     3541 gcacttcttc ttcaggtcat tacattagtg atgtatatga cattaagaag caagcgtggt
     3601 ttacttacaa tgacctggag gtatcaaaaa tccaagaggc tgccgtgcag agtgatcgag
     3661 atcggagtgg ctacatcttc ttttatatgc acaaggagat ctttgatgag ctgctggaaa
     3721 cagaaaagaa ctctcagtca cttagcacgg aagtggggaa gactacccgt caggcctcgt
     3781 gatacccaac tttcttgtac aaagtggttc gataacgaat tctaccgggt aggggaggcg
     3841 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     3901 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     3961 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     4021 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     4081 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     4141 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     4201 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     4261 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     4321 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4381 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4441 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4501 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     4561 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     4621 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     4681 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     4741 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     4801 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     4861 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     4921 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     4981 cccatgcatc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     5041 accccacctg taggtttggc aagctagaga accatcagat gtttccaggg tgccccaagg
     5101 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     5161 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca
     5221 gtcctccgat agactgcgtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
     5281 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
     5341 tcagcggggg tctttcatgg gtaacagttt cttgaagttg gagaacaaca ttctgagggt
     5401 aggagtcgaa tattaagtaa tcctgactca attagccact gttttgaatc cacatactcc
     5461 aatactcctg aaatagttca ttatggacag cgcagaaaga gctggggaga attgtgaaat
     5521 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5581 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5641 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5701 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5761 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5821 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5881 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5941 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6001 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6061 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6121 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6181 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6241 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6301 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6361 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6421 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6481 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6541 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6601 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6661 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6721 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6781 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6841 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6901 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6961 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7021 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7081 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7141 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7201 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7261 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7321 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7381 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7441 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7501 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7561 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7621 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     7681 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     7741 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     7801 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     7861 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     7921 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     7981 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     8041 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     8101 acgacggcgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
     8161 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     8221 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     8281 tgccggccac gatgcgtccg gcgtagaggc gattagtcca atttgttaaa gacaggatat
     8341 cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg
     8401 agccatagat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     8461 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaatac
     8521 ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc agaatatggg
     8581 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     8641 ccccagatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     8701 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     8761 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     8821 cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt
     8881 gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc agattgattg
     8941 actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag acccctgccc
     9001 agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc gtgtctgtct
     9061 ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg tactagttag
     9121 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc
     9181 gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa
     9241 gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc
     9301 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt
     9361 cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt
     9421 atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact
     9481 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
     9541 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
     9601 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
     9661 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
     9721 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
     9781 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
     9841 cttctctagg cgccggaatt agatcctt
//
