LOCUS       sequence-313976-        4572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-MAP3K11
FEATURES             Location/Qualifiers
     source          1..4572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(22..108)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     157..179
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     171..188
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     172..188
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    204..303
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2086..2185)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2202..2221)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2203..2221)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2226..2242)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2226..2242)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2673..3464
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3557..4145
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4046..4065
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4299..4316
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4475..4502)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
       61 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
      121 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
      181 cggccagtct taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt
      241 cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      301 gcaccatgga gcccttgaag agcctcttcc tcaagagccc tctagggtca tggaatggca
      361 gtggcagcgg gggtggtggg ggcggtggag gaggccggcc tgaggggtct ccaaaggcag
      421 cgggttatgc caacccggtg tggacagccc tgttcgacta cgagcccagt gggcaggatg
      481 agctggccct gaggaagggt gaccgtgtgg aggtgctgtc ccgggacgca gccatctcag
      541 gagacgaggg ctggtgggcg ggccaggtgg gtggccaggt gggcatcttc ccgtccaact
      601 atgtgtctcg gggtggtggc ccgcccccct gcgaggtggc cagcttccag gagctgcggc
      661 tggaggaggt gatcggcatt ggaggctttg gcaaggtgta caggggcagc tggcgaggtg
      721 agctggtggc tgtgaaggca gctcgccagg accccgatga ggacatcagt gtgacagccg
      781 agagcgttcg ccaggaggcc cggctcttcg ccatgctggc acaccccaac atcattgccc
      841 tcaaggctgt gtgcctggag gagcccaacc tgtgcctggt gatggagtat gcagccggtg
      901 ggcccctcag ccgagctctg gccgggcggc gcgtgcctcc ccatgtgctg gtcaactggg
      961 ctgtgcagat tgcccgtggg atgcactacc tgcactgcga ggccctggtg cccgtcatcc
     1021 accgtgatct caagtccaac aacattttgc tgctgcagcc cattgagagt gacgacatgg
     1081 agcacaagac cctgaagatc accgactttg gcctggcccg agagtggcac aaaaccacac
     1141 aaatgagtgc cgcgggcacc tacgcctgga tggctcctga ggttatcaag gcctccacct
     1201 tctctaaggg cagtgacgtc tggaggtgcg gcccttggtg gggctggacg ggctgctacg
     1261 gggctgcaga gggcagaggt ggctgcctaa atctagggct gggccaagtc cagggacagg
     1321 gacccaaacc acttggctcc tagttgcttt tgggtgaagt ggcagtaagg gggtgggggt
     1381 tgggagagaa aagggaacac agacctgttg tctcgcccac agttttgggg tgctgctgtg
     1441 ggaactgctg accggggagg tgccataccg tggcattgac tgccttgctg tggcctatgg
     1501 cgtagctgtt aacaagctca cactgcccat cccatccacc tgccccgagc ccttcgcaca
     1561 gcttatggcc gactgctggg cgcaggaccc ccaccgcagg cccgaccgag ccgccccctt
     1621 ccccgctcat ctgcttctcg ctcaagacgc ccgactcccc gcccactcct gcacccctgt
     1681 tgctggacct gggtatccct gtgggccagc ggtcagccaa gagcccccga cgtgaggagg
     1741 agccccgcgg aggcactgtc tcacccccac cggggacatc acgctctgct cctggcaccc
     1801 caggcacccc acgttcacca cccctgggcc tcatcagccg acctcggccc tcgccccttc
     1861 gcagccgcat tgatccctgg agctttgtgt cagctgggcc acggccttct cccctgccat
     1921 caccacagcc tgcaccccgc cgagcaccct ggaccttgtt cccggactca gaccccttct
     1981 gggactcccc acctgccaac cccttccagg ggggccccca ggactgcagg gcacagacca
     2041 aagacatggg tgcccaggcc ccgtgggtgc cggaagcggg gcctttgcca actttcttgt
     2101 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     2161 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     2221 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     2281 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
     2341 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     2401 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     2461 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2521 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     2581 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     2641 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     2701 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     2761 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     2821 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2881 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     2941 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     3001 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     3061 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     3121 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     3181 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     3241 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     3301 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     3361 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     3421 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     3481 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     3541 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3601 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3661 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3721 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3781 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3841 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3901 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3961 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4021 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4081 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4141 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4201 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4261 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4321 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4381 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     4441 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     4501 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     4561 ttgcttcaca ac
//
