LOCUS       sequence-31398-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SGF29 (3MET)
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(576..587)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             662..679
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(732..750)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      746..793
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tccctctgaa tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggccgcag aggggtgctg atgaccctgc
      121 tgcagcagtc ggccatgacc ctgcccctgt ggatcgggaa gcctggtgac aagcccccac
      181 ccctctgtgg ggccatccct gcctcaggag actacgtggc cagacctgga gacaaggtgg
      241 ctgcccgggt gaaggccgtg gatggggacg agcagtggat cctggccgag gtggtcagtt
      301 acagccatgc caccaacaag tatgaggtag atgacatcga tgaagaaggc aaagagagac
      361 acaccctgag ccggcgccgt gtcatcccgc tgccccagtg gaaggccaac ccggagacgg
      421 accctgaggc cttgttccag aaggagcagc tcgtgctggc cctgtatccc cagactacct
      481 gcttctaccg cgccctgatc catgcgcccc cacagcggcc ccaggatgac tactcggtcc
      541 tgtttgaaga cacctcctat gcagatggct attcccctcc cctcaatgtg gctcagagat
      601 acgtggtggc ttgtaaggaa cccaagaaaa agtgatgacg aagcttgcgg ccgcactcga
      661 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      721 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      781 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      841 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      901 agcgccctag cgcccgctcc tttcgcttct cct
//
