LOCUS       sequence-31413-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBX5 (3FDT)
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             290..307
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(360..378)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      374..421
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      458..838
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(545..564)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     755..776
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat cacagcagcg
       61 gcagagaaaa cttgtatttc cagggcgagg agtatgttgt ggagaaggtg ctagacaggc
      121 gcgtggttaa gggacaagtg gaatatctac tgaagtggaa aggcttttct gaggagcaca
      181 atacttggga acctgagaaa aacttggatt gccctgagct aatttctgaa tttatgaaaa
      241 agtataagaa gatgaaggag tgatgacgaa gcttgcggcc gcactcgagc accaccacca
      301 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      361 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      421 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      481 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      541 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      601 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      661 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      721 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      781 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
      841 tattggttaa aaaatgagct gatttaacaa aaatttaacg cg
//
