LOCUS       sequence-31419-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CENTA1 (3FM8)
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..692
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     650..672
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             720..737
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             747..764
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atatacatat gtcccctata ctaggttatt
       61 ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat
      121 atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat
      181 tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt
      241 ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag
      301 agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga
      361 gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg
      421 aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg
      481 taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa
      541 tgtgcctgga tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac
      601 aaattgataa gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag
      661 ccacgtttgg tggtggcgac catcctccaa aatcggatgg ttcatccatg ggcagcagcc
      721 atcatcatca tcatcacagc agcggcctgg ttccgcgtgg tagtatggcc aaggagcggc
      781 gcagggcggt cctggagctg ctgcagcggc cggggaacgc gcgctgcgcg gactgcggcg
      841 ccccggatcc cgactgggcc tcctacactc tgggcgtctt catctgcctg agctgctc
//
