LOCUS       sequence-31420-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RRAGC
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             651..668
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(721..739)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      735..782
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atattttgtt taactttaag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggctcc aagccgagga ttctgctcat gggactccgg
      121 cgcagcggca agtcctccat ccagaaggtg gtgtttcata agatgtcacc caacgagacc
      181 ctctttttgg aaagtaccaa caagatttat aaggatgaca tttccaatag ctcctttgtg
      241 aatttccaga tatgggattt tcctgggcaa atggactttt ttgacccaac ctttgactat
      301 gagatgatct tcaggggaac aggagcattg atatacgtca ttgacgcaca ggatgactac
      361 atggaggctt taacaagact tcacattact gtttctaaag cctacaaagt taacccagac
      421 atgaattttg aggtttttat tcacaaagtt gatggtctgt ctgatgatca caaaatagaa
      481 acacagaggg acattcatca aagggccaat gatgaccttg cagatgctgg gctagaaaaa
      541 ctccatctta gcttttatct gactagtatc tatgaccatt caatatttga agcctttagt
      601 aaggtggtgc agaaactcat ttgatgacga agcttgcggc cgcactcgag caccaccacc
      661 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      721 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      781 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      841 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      901 gcccgctcct ttcgcttctc ctctt
//
