LOCUS       sequence-31432-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HECW1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             535..552
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(605..623)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      619..666
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(790..809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaattcctct gaatattttg tttactttag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggctca gaagccgaat cttcccagtc
      121 cagcttagat ctaaggagag aggggtcact ttctccagtg aactcacaaa aaatcacctt
      181 gctgctgcag tccccagcgg tcaagttcat caccaacccc gagttcttca ctgtgctaca
      241 tgccaattat agtgcctacc gagtcttcac cagtagcacc tgcttaaagc acatgattct
      301 gaaagtccga cgggatgctc gcaattttga acgctaccag cacaaccggg acttggtgaa
      361 tttcatcaac atgttcgcag acactcggct ggaactgccc cggggctggg agatcaaaac
      421 ggaccagcag ggaaagtctt ttttcgtgga ccacaacagt cgagctacca ctttcattga
      481 cccccgaatc cctcttcaga acggttgatg acgaagcttg cggccgcact cgagcaccac
      541 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      601 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      661 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
      721 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      781 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      841 gtcaagctct aaatc
//
