LOCUS       sequence-314419-        3723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-coding DNA sequence for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Davey CA
  TITLE     Near-atomic resolution structures of interdigitated nucleosome 
            fibres.
  JOURNAL   Nat Commun. 2020 Sep 21;11(1):4747. doi: 10.1038/s41467-020-18533-2.
  PUBMED    32958761
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-21"; volume: "11(1):4747. doi"; pages: " 
            10.1038/s41467-020-18533-2"
COMMENT     Sequence Label: Sticky-349-3X
FEATURES             Location/Qualifiers
     source          1..3723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1327..1344)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1327..1343)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1336..1358)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1552..1571)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1671..1693
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1731..1749)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1817..1921
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1922..1990,1991..2782)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2140..2159)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2953..3541
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3442..3461
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3695..3712
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
      241 agatctggta ccgctggttt tttttttcat gtgccggtct cacacgtgcc tggagactag
      301 taagcgcttc tagtggcggt taaaacgcgg tagacagcgc gtacgtgcgt ttaagcggtg
      361 ctagagctgt ctacgaccaa ttgagcggcc tcggcaccgg gatgcgtttt ttttttcata
      421 ctcgagcatg cttttttttt tcatgtgccg gtctcacacg tgcctggaga ctagtaagcg
      481 cttctagtgg cggttaaaac gcggtagaca gcgcgtacgt gcgtttaagc ggtgctagag
      541 ctgtctacga ccaattgagc ggcctcggca ccgggatgcg tttttttttt ccagcggtac
      601 cgctggtttt ttttttcatg tgccggtctc acacgtgcct ggagactagt aagcgcttct
      661 agtggcggtt aaaacgcggt agacagcgcg tacgtgcgtt taagcggtgc tagagctgtc
      721 tacgaccaat tgagcggcct cggcaccggg atgcgttttt tttttcatac tcgagcatgc
      781 tttttttttt catgtgccgg tctcacacgt gcctggagac tagtaagcgc ttctagtggc
      841 ggttaaaacg cggtagacag cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac
      901 caattgagcg gcctcggcac cgggatgcgt tttttttttc cagcggtacc gctggttttt
      961 tttttcatgt gccggtctca cacgtgcctg gagactagta agcgcttcta gtggcggtta
     1021 aaacgcggta gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt
     1081 gagcggcctc ggcaccggga tgcgtttttt ttttcatact cgagcatgct tttttttttc
     1141 atgtgccggt ctcacacgtg cctggagact agtaagcgct tctagtggcg gttaaaacgc
     1201 ggtagacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg
     1261 cctcggcacc gggatgcgtt ttttttttcc agcggtaccg gatccccggg taccgagctc
     1321 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     1381 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     1441 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     1501 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     1561 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     1621 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     1681 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     1741 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1801 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1861 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1921 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1981 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2041 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2101 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2161 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     2221 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2281 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2341 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2401 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2461 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     2521 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2581 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2641 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2701 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2761 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2821 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2881 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2941 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3001 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3061 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3121 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3181 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3241 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3301 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3361 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3421 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3481 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3541 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3601 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3661 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3721 aga
//
