LOCUS       sequence-31442-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RNF128
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     90..152
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             165..182
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             204..224
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gtttcgtaca gttttgtata aaaaaaccta taaatattcc ggattattca taccgtccca
       61 ccatcgggcg cggatctcgg tccgaaacca tgaaattctt agtcaacgtt gcccttgttt
      121 ttatggtcgt atacatttct tacatctatg cggccgctcc ggaacatcac catcaccatc
      181 acgattacga tatcccaacg accgaaaacc tgtattttca gggcgccatg gatggggctg
      241 aagcagtgtg gaccgcgtac ctcaacgtgt cctggcgggt tccgcacacg ggagtgaacc
      301 gtacggtgtg ggagctgagc gaggagggcg tgtacggcca ggactcgccg ctggagcctg
      361 tggctggggt cctggtaccg cccgacgggc ccggggcgct taacgcctgt aacccgcaca
      421 cgaatttcac ggtgcccacg gtttggggaa gcaccgtgca agtctcttgg ttggccctca
      481 tccaacgcgg cgggggctgc accttcgcag acaagatcca tctggcttat gagagagggg
      541 cgtctggagc cgtcatcttt aacttccccg ggacccgcaa tgaggtcatc cccatgtctc
      601 acccgggtgc agtagacatt gttgcaatca tgatcggcaa tctgaaaggc acaaaaattc
      661 tgcaatctat tcaaagaggc atacaagtga caatggtcat agaagtaggg aaaaaacatg
      721 gcccttgggt gaattgatga agcttgtcga gaagtactag aggatcataa tcagccatac
      781 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      841 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      901 at
//
