LOCUS       sequence-314535-        9225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 spike protein with a D614G mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang
            Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, 
            Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, 
            Kyratsous CA, Dudkina NV, Shen K, Luban J
  TITLE     Structural and Functional Analysis of the D614G SARS-CoV-2 Spike 
            Protein Variant.
  JOURNAL   Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032.
  PUBMED    32991842
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032."
COMMENT     Sequence Label: pcDNA3.1 SARS-CoV-2 S D614G
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4816..4833)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4822..5046
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5092..5520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5179..5198)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5389..5410
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5529..5549)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5534..5863
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5714..5849
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5776..5795
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5930..6724
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5984..6003)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6594..6613
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6898..7019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6935..6954)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6989..7008
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7068..7084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7068..7084)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7081..7103)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7092..7108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7116..7122,7123..7140,7141..7146))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7161..7182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7299..7316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7470..8058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7550..7569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8229..9020,9021..9089))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8852..8871
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9090..9194)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga gccaccatgt ttgtgttctt ggtcttgttg cctttggttt
      961 caagtcagtg cgtgaatttg actacacgaa ctcagctgcc gcctgcctac accaattcct
     1021 tcacgcgagg ggtctactac ccagataaag tttttcggag tagtgttctc cactctaccc
     1081 aggacctttt cctccccttc tttagcaacg tcacatggtt ccatgcaata cacgttagcg
     1141 ggacgaatgg gaccaaacgc ttcgataatc cggtactccc gttcaatgat ggggtctact
     1201 tcgcttctac tgagaagtca aacattatcc gcggttggat attcggcaca acacttgatt
     1261 ctaaaactca gtctctcctg attgtgaata atgctaccaa tgttgtgatt aaggtctgcg
     1321 agttccaatt ttgcaatgac ccgttcctgg gcgtttatta tcataagaat aacaagagct
     1381 ggatggagtc tgagttccgc gtatactctt ctgccaataa ttgtaccttc gaatacgtca
     1441 gtcagccgtt cttgatggac ctcgaaggaa agcaaggcaa tttcaaaaac ctcagggagt
     1501 tcgtttttaa gaatatcgat ggttacttta agatatattc aaaacataca cccataaacc
     1561 ttgtccgaga tctgccgcaa ggatttagtg cacttgaacc gctggtcgat cttcctattg
     1621 gaattaacat tacccggttt caaactctgt tggctttgca ccggagttat cttacgccag
     1681 gcgacagctc cagtggatgg acggcagggg ctgctgcata ctatgtcggc taccttcaac
     1741 cccggacttt tctcctcaag tacaacgaga atgggaccat aacagacgct gtagattgtg
     1801 ctctggatcc ccttagcgaa acaaagtgca cgctcaagtc ctttacggtt gaaaaaggga
     1861 tctatcagac cagtaatttc cgcgtacaac ccaccgaaag cattgtacgg tttccgaaca
     1921 ttaccaatct gtgtcctttc ggtgaggttt tcaatgcgac ccggttcgca tcagtctacg
     1981 catggaaccg caagcgcatt agcaattgtg tcgccgatta tagcgtgctt tacaattctg
     2041 cctctttctc cacattcaag tgttatggtg tgtcacctac taagctcaat gacctgtgtt
     2101 ttacaaatgt ttacgccgac agctttgtca ttaggggcga tgaggtccgc cagattgcgc
     2161 ctgggcagac gggaaaaata gccgattata actataagct tcctgatgat tttaccggct
     2221 gtgtgatagc ctggaactca aacaatcttg attctaaagt gggaggaaac tataactatc
     2281 tctaccggct ctttcgcaag tccaacctca aaccgttcga gcgagacata agcacagaaa
     2341 tctaccaagc aggtagtacg ccgtgcaacg gggtagaagg gtttaattgt tacttcccgt
     2401 tgcagagtta cggatttcaa ccaaccaacg gagtagggta tcagccgtac cgcgttgtgg
     2461 ttctctcatt cgaacttctg catgcacccg cgacagtatg tggtccaaag aagtctacga
     2521 acctcgttaa gaataaatgc gtgaacttta actttaatgg cctgacaggg acgggggtcc
     2581 ttaccgagag caacaagaaa tttttgccgt tccagcagtt tgggagggat attgctgaca
     2641 ctaccgacgc agttcgcgac ccacagactc tggaaatact tgacatcaca ccttgctcat
     2701 ttggaggggt ctccgtcata acccctggta ctaacacatc taatcaagtt gctgttctct
     2761 atcagggtgt aaattgtaca gaggttcctg tagccatcca tgctgaccag ctgacaccca
     2821 cctggagggt ttatagtacc ggaagcaacg ttttccaaac tagagcaggt tgtttgattg
     2881 gtgctgagca cgtaaataac tcatacgaat gcgacatacc tattggagcg ggtatctgtg
     2941 caagctacca aactcaaacc aattcaccga ggcgcgcacg atccgttgcg agccaatcca
     3001 taattgccta cacgatgagc ctcggcgcag aaaactcagt tgcgtattcc aataacagca
     3061 ttgcaatccc tacaaatttt acgatcagtg ttacgaccga aatcctgccg gtatcaatga
     3121 cgaaaacaag cgtggactgc accatgtaca tctgtgggga tagtaccgag tgtagtaact
     3181 tgctgctcca gtatgggtca ttttgcactc aactcaacag agctttgaca ggcatcgccg
     3241 ttgaacaaga taaaaatacc caagaagtat ttgctcaagt caaacaaatt tacaagactc
     3301 ctcccataaa agactttgga gggttcaact tttcccagat attgccggac ccatcaaagc
     3361 caagcaagcg gtcatttatt gaagacttgc tcttcaacaa agttacactt gctgacgcgg
     3421 gattcattaa acagtatggt gactgtttgg gagatattgc ggcgcgggac ctgatatgtg
     3481 ctcaaaagtt caatgggctg acggtgctgc ccccgctgct tacggacgaa atgattgccc
     3541 aatacacatc tgctctcctt gccggtacca ttacttcagg gtggacgttc ggcgcaggag
     3601 ctgcccttca aatacctttt gcaatgcaaa tggcatatcg gttcaacgga ataggtgtca
     3661 cgcagaacgt attgtacgag aaccaaaagc tcattgcaaa tcaatttaac tcagcgatcg
     3721 gtaagataca ggactcactt agctctaccg cttccgccct ggggaagctg caagatgtcg
     3781 taaaccagaa cgctcaagcg cttaatacat tggttaaaca attatctagc aattttggtg
     3841 ctatcagttc cgttcttaac gacattttgt ctcggcttga caaggttgag gcggaagttc
     3901 aaatcgacag actcataaca ggacggctgc agtccctgca aacgtatgtc actcaacagc
     3961 tcattcgggc cgcggagatt cgggcaagcg caaaccttgc ggccacaaaa atgagtgaat
     4021 gtgtacttgg ccagtccaag agagttgatt tctgtggaaa aggataccac ctgatgtcct
     4081 tcccgcaatc tgcgccgcac ggcgttgttt ttttgcatgt aacttatgta cctgcgcaag
     4141 aaaaaaattt cacgactgcg cctgcaatat gtcatgacgg gaaggctcac ttcccaaggg
     4201 aaggcgtctt tgtatcaaat gggacgcatt ggttcgtcac gcagcgcaac ttttatgagc
     4261 cccagattat aactaccgac aacacattcg tcagcggcaa ttgtgatgtg gtaatcggca
     4321 tagtcaacaa taccgtatac gatcctttgc aacctgaact tgactcattc aaggaggaat
     4381 tggataagta tttcaaaaat catacctctc cagacgtcga tctcggtgat atatcaggta
     4441 taaatgcgtc agtcgtaaac atccagaagg agattgatcg cttgaacgag gttgctaaga
     4501 atttgaatga aagcctcatc gacctccagg aattagggaa atatgaacaa tacattaagt
     4561 ggccttggta catctggttg gggttcatcg caggactcat cgcgatcgtg atggttacca
     4621 taatgctttg ttgcatgacg agctgttgtt cctgtctgaa aggttgctgc tcttgcggca
     4681 gttgctgcaa gtttgatgag gacgattctg aaccagttct caaaggggta aaattgcatt
     4741 acacataaga attccaccac actggactag tggatccgag ctcggtacca agcttaagtt
     4801 taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4861 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4921 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4981 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     5041 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     5101 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5161 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5221 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5281 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5341 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5401 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5461 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5521 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5581 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5641 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5701 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5761 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     5821 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     5881 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     5941 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     6001 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     6061 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     6121 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6181 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6241 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6301 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6361 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6421 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     6481 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     6541 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6601 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     6661 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     6721 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     6781 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     6841 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     6901 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6961 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7021 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     7081 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7141 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7201 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7261 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7321 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7381 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7441 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7501 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7561 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7621 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7681 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7741 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7801 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7861 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7921 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7981 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8041 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8101 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8161 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8221 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8281 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8341 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8401 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8461 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8521 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8581 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8641 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8701 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8761 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8821 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8881 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8941 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9001 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9061 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9121 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9181 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
