LOCUS       sequence-314603-       10541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDKL1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10541)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDKL1_IDG-K
FEATURES             Location/Qualifiers
     source          1..10541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    158..274
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    308..328
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        328..1539
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1376..1395
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1543..1563)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1564..1573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1576..2346
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2353..2394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2395..2419
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3503..3527)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3557..4145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3610..3630)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4028..4039)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4159..4658
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4194..4217)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4591..4610
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4679..5278
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4679..4698)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5175..5195
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5416..5649
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5721..5842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5758..5777)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5812..5831
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5882..6017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6037..6056)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6038..6056)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6066..6083)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6066..6082)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6075..6097)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6224..6679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6311..6330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6521..6542
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6705..6809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6810..6878,6879..7670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7841..8429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8330..8349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8583..8600
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8717..8738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8753..8758,8759..8776,8777..8783)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8791..8807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8796..8818
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8815..8831
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8815..8831
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8850..8870
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8852..8870
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8898..9124
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9125..9305
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9352..9477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9970..10203
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10388..10432
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
       61 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
      121 ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg
      181 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      241 aattacaaaa acaaattaca aaattcaaaa ttttatcgtc cagtgtggtg gaattctgca
      301 gatatcacaa ctttgtatag aaaagttggc ctccgcgccg ggttttggcg cctcccgcgg
      361 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
      421 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
      481 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
      541 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
      601 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
      661 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
      721 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
      781 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
      841 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
      901 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
      961 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
     1021 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
     1081 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
     1141 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
     1201 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
     1261 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     1321 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     1381 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     1441 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     1501 tccgctaaat tctggccgtt tttggctttt ttgttagacg gccaactttt gtatacaaag
     1561 tgggccacca tgggaatccc gctgctgaac gctaaacaga ttctgggaca gctggacggc
     1621 gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct ggatcgaatc
     1681 ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg gagaggaagc
     1741 agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat gttttggcgc
     1801 ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt catggcagaa
     1861 gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga cctgtatctg
     1921 caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg cgatgctgca
     1981 cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga aagcgtggtc
     2041 aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa tactctggcc
     2101 gctatgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga aggcctggct
     2161 ccatatctgt cacggtggga gaagctggat aacttcatca atagacccgt gaagctgatc
     2221 attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg agccctgctg
     2281 ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct gcggtctgcc
     2341 gaaaaggcta gcggcaagcc catccccaac cccctgctgg gcctggacag caccacaact
     2401 ttgtacaaaa aagcaggctc caccatgatg gagaagtatg aaaaaattgg gaaaattgga
     2461 gaaggatcct atggagttgt tttcaaatgt agaaacaggg acacgggtca gattgtggcc
     2521 atcaagaagt ttctggaatc agaagatgac cctgtcataa agaaaattgc ccttcgggaa
     2581 atccgaatgc tcaagcaact caagcatccc aaccttgtta acctcctgga agtcttcagg
     2641 aggaaacgga ggcttcacct ggtgtttgaa tattgtgacc acacagttct ccatgagttg
     2701 gacagatacc aaagaggggt accagaacat ctcgtgaaga gcataacttg gcagacactg
     2761 caagctgtaa atttttgcca taaacacaat tgcatacata gagacgtgaa gccagaaaat
     2821 atcctcatca cgaaacattc cgtgattaag ctttgtgact ttggatttgc tcggcttttg
     2881 actggaccga gtgactacta tacagactac gtggctacca ggtggtaccg ctcccctgag
     2941 ctgctggtgg gggacacgca gtacggcccc ccggtggatg tttgggcaat tggctgtgtc
     3001 tttgctgagc tgctgtcagg agtgcctctg tggccaggaa aatcggatgt ggatcagctg
     3061 tatctgatta ggaagacctt gggggatctc attcctaggc accagcaagt gtttagcacg
     3121 aatcagtact tcagtggagt gaaaattcca gaccctgaag atatggaacc acttgaatta
     3181 aaattcccaa acatctctta tcctgccctg gggctcctaa agggctgtct ccacatggac
     3241 cctactcaaa ggctgacatg tgaacagctg ttgcatcacc catattttga aaacatcaga
     3301 gaaatagagg atttggcaaa agaacacaac aaaccaacaa ggaagaccct aagaaagagc
     3361 cgaaagcacc actgctttac agaaacatcc aagttgcagt acctacccca gctaactggc
     3421 agcagcatcc ttccagcttt ggataataag aagtactact gtgataccaa gaaacttaac
     3481 taccgttttc caaacattta ggacccagct ttcttgtaca aagttgtgat atccagcaca
     3541 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3601 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3661 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3721 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3781 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3841 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3901 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     3961 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4021 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4081 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4141 cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     4201 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     4261 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     4321 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     4381 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     4441 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     4501 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     4561 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     4621 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     4681 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4741 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4801 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4861 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4921 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4981 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5041 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5101 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5161 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5221 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     5281 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg
     5341 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5401 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg
     5461 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5521 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5581 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5641 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5701 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5761 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5821 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5881 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5941 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6001 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     6061 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6121 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6181 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     6241 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6301 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6361 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6421 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6481 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6541 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6601 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6661 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7981 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8581 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8641 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8701 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8761 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8821 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     8881 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     8941 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     9001 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     9061 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     9121 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     9361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     9421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     9481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     9541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     9781 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     9841 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     9901 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9961 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    10021 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    10081 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    10141 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    10201 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    10261 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    10321 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    10381 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    10441 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    10501 ggtaggttta agaatagttt ttgctgtact ttctatagtg a
//
