LOCUS       sequence-31464-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: COMTD1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             800..817
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(870..888)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggttccg cgtggtagtc ccccatggcg aggccggcga gagcagtgcc
      121 tgcttccccc cgaggacagc cgcctgtggc agtatcttct gagccgctcc atgcgggagc
      181 acccggcgct gcgaagcctg aggctgctga ccctggagca gccgcagggg gattctatga
      241 tgacctgcga gcaggcccag ctcttggcca acctggcgcg gctcatccag gccaagaagg
      301 cgctggacct gggcaccttc acgggctact ccgccctggc cctggccctg gcgctgcccg
      361 cggacgggcg cgtggtgacc tgcgaggtgg acgcgcagcc cccggagctg ggacggcccc
      421 tgtggaggca ggccgaggcg gagcacaaga tcgacctccg gctgaagccc gccttggaga
      481 ccctggacga gctgctggcg gcgggcgagg ccggcacctt cgacgtggcc gtggtggatg
      541 cggacaagga gaactgctcc gcctactacg agcgctgcct gcagctgctg cgacccggag
      601 gcatcctcgc cgtcctcaga gtcctgtggc gcgggaaggt gctgcaacct ccgaaagggg
      661 acgtggcggc cgagtgtgtg cgaaacctaa acgaacgcat ccggcgggac gtcagggtct
      721 acatcagcct cctgcccctg ggcgatggac tcaccttggc cttcaagatc tgatgacgaa
      781 gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      841 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      901 ggcctctaaa c
//
