LOCUS       sequence-314663-       11363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of ICK (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11363)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-ICK_IDG-K
FEATURES             Location/Qualifiers
     source          1..11363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    55..171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    205..225
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        225..1436
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1273..1292
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1440..1460)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1461..1470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1473..2243
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2250..2291
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2292..2316
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             3260..3268
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(4222..4246)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4276..4864
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4329..4349)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4747..4758)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4878..5377
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4913..4936)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5310..5329
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5398..5997
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5398..5417)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5894..5914
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6135..6368
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6440..6561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6477..6496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6531..6550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6601..6736
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6756..6775)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6757..6775)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6785..6802)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6785..6801)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6794..6816)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6943..7398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7030..7049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7240..7261
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7424..7528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7529..7597,7598..8389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8560..9148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9049..9068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9302..9319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9436..9457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9472..9477,9478..9495,9496..9502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9510..9526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9515..9537
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9534..9550
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9534..9550
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9569..9589
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9571..9589
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9617..9843
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9844..10024
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10071..10196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10689..10922
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11107..11151
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
       61 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      121 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag
      181 tgtggtggaa ttctgcagat atcacaactt tgtatagaaa agttggcctc cgcgccgggt
      241 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
      301 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
      361 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
      421 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
      481 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
      541 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
      601 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
      661 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
      721 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
      781 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
      841 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
      901 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
      961 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     1021 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     1081 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     1141 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     1201 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     1261 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     1321 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     1381 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacggcc
     1441 aacttttgta tacaaagtgg gccaccatgg gaatcccgct gctgaacgct aaacagattc
     1501 tgggacagct ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt
     1561 acctgctgga tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc
     1621 aggcagggag aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc
     1681 tgagtatgtt ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg
     1741 gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc
     1801 ccaatgacct gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa
     1861 taacaggcga tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg
     1921 tggaggaaag cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg
     1981 acaggaatac tctggccgct atgctgatcc gagagctgcg ggcagccctg gaactgttcg
     2041 agcaggaagg cctggctcca tatctgtcac ggtgggagaa gctggataac ttcatcaata
     2101 gacccgtgaa gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata
     2161 aacagggagc cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa
     2221 tcagtctgcg gtctgccgaa aaggctagcg gcaagcccat ccccaacccc ctgctgggcc
     2281 tggacagcac cacaagtttg tacaaaaaag caggctccac catgaataga tacacaacaa
     2341 tcaggcagct cggggatgga acctacggtt ccgtcctgct gggaagaagc attgagtctg
     2401 gggagctgat cgctattaaa aaaatgaaaa gaaaatttta ttcctgggag gaatgcatga
     2461 accttcggga ggttaagtct ttaaagaagc tcaaccatgc caatgtagtc aaattaaaag
     2521 aagttatcag ggaaaatgat catctttatt ttatcttcga gtacatgaag gaaaatcttt
     2581 accagctcat taaagagaga aataagttgt ttcctgagtc tgctataagg aatatcatgt
     2641 atcagatatt acaaggactc gcatttattc acaaacacgg cttctttcat cgagacttaa
     2701 agcctgagaa cctcctctgc atgggaccag aacttgtgaa aattgcagac tttggtttgg
     2761 cccgagaaat acgatcaaaa cctccatata cagattatgt atctaccaga tggtacaggg
     2821 ctccagaagt actcctgagg tctaccaact acagctcccc cattgacgtc tgggcggtgg
     2881 gctgcatcat ggcagaagtt tacaccctca ggccactctt ccctggagcc agtgaaattg
     2941 acacaatatt caaaatttgc caagtgctgg ggacaccaaa aaagactgac tggcctgaag
     3001 gctatcaact ttcaagtgca atgaacttcc gttggccaca gtgtgtaccc aataacttaa
     3061 agaccttgat tcccaatgct agcagtgaag cagtccagct cctgagagac atgcttcagt
     3121 gggatcccaa gaaacgacca acagctagtc aggcacttcg atatccttac ttccaagttg
     3181 gacacccact aggcagcacc acacaaaacc ttcaggattc agaaaaacca cagaaaggca
     3241 tcctggaaaa ggcaggccca cctccttata ttaagccagt cccacctgcc cagccgccag
     3301 ccaagccaca cacacgaatt tcttcacgac agcatcaagc cagccagccc cctctgcatc
     3361 tcacgtaccc ctacaaagca gaggtctcca ggacagatca cccaagccat ctccaggagg
     3421 acaagccaag cccgttgctt ttcccatccc tccacaacaa gcatccacag tcgaaaatca
     3481 cagctggcct ggagcacaaa aatggtgaga taaagccaaa gagtaggaga aggtggggtc
     3541 ttatttccag gtcaacaaag gattcagatg attgggctga cttggatgac ttggatttca
     3601 gtccatccct cagcaggatt gacctgaaaa acaagaaaag acagagtgat gacactctct
     3661 gcaggtttga gagtgttttg gacctgaagc cctctgagcc tgtgggcaca ggaaacagtg
     3721 cccccaccca gacgtcatat cagcggcgag acacgcccac cctgagatct gcagccaagc
     3781 agcactattt gaagcactct cgatacttgc ctgggatcag tataagaaat ggcatactct
     3841 cgaatccagg caaggaattt attccaccta atccatggtc tagttctggc ttgtctggaa
     3901 aatcttcagg gacaatgtca gtaatcagca aagtaaattc agttggttcc agctctacaa
     3961 gttctagtgg actgactgga aactatgtcc cttcctttct gaaaaaagaa atcggttctg
     4021 ctatgcagag ggtacaccta gcacctattc cagacccttc ccctggttat tcctccctga
     4081 aggccatgag acctcatcct gggcgaccat tcttccacac ccagcctaga agcactcctg
     4141 ggttgatacc acggcctcca gccgcccagc cagtgcatgg ccggacagac tgggcttcca
     4201 agtacgcatc tcggcgatag gacccagctt tcttgtacaa agtggtgata tccagcacag
     4261 tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4321 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4381 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4441 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4501 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4561 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4621 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     4681 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4741 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4801 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4861 ccgcctggaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     4921 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     4981 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     5041 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     5101 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     5161 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     5221 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     5281 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     5341 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg
     5401 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     5461 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     5521 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5581 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     5641 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5701 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5761 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5821 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5881 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5941 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     6001 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atctcgaggg
     6061 cccggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6121 agaaaagggg ggactggaag ggctagctca ctcccaacga agacaagatc tgctttttgc
     6181 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6241 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6301 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6361 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     6421 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     6481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6541 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     6601 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6661 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     6721 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     6781 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     6841 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     6901 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     6961 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7021 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7081 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7141 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7201 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7261 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7321 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     7381 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7441 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7501 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7561 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7621 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7681 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7741 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7801 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7861 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7921 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7981 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8041 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8101 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8161 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8221 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8281 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8341 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8401 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8461 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8701 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9181 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9241 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9301 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     9361 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     9421 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     9481 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9541 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     9601 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     9661 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     9721 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     9781 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     9841 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     9901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     9961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    10021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
    10081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
    10141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
    10201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
    10261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
    10321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
    10381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
    10441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
    10501 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
    10561 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    10621 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    10681 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    10741 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    10801 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    10861 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    10921 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    10981 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    11041 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    11101 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    11161 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    11221 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    11281 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    11341 atagaagaag aaggtggaga gag
//
