LOCUS       sequence-31470-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SRM
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attccctctg aatattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggttc cgcgtggtag tatggagccc ggccccgacg
      121 gccccgccgc ctccggcccc gccgccatcc gcgagggctg gttccgcgag acctgcagcc
      181 tgtggcccgg ccaggccctg tcgctgcagg tggagcagct gctccaccac cggcgctcgc
      241 gctaccagga catcctcgtc ttccgcagta agacctatgg caacgtgctg gtgttggacg
      301 gtgtcatcca gtgcacggag agagacgagt tctcctacca ggagatgatc gccaacctgc
      361 ctctctgcag ccaccccaac ccgcgaaagg tgctgatcat cgggggcgga gatggaggtg
      421 tcctgcggga ggtggtgaag cacccctccg tggagtccgt ggtccagtgt gagatcgacg
      481 aggatgtcat ccaagtctcc aagaagttcc tgccaggcat ggccattggc tactctagct
      541 cgaagctgac cctacatgtg ggtgacggtt ttgagttcat gaaacagaat caggatgcct
      601 tcgacgtgat catcactgac tcctcagacc ccatgggccc cgccgaaagt ctcttcaagg
      661 agtcctatta ccagctcatg aagacagccc tcaaggaaga tggtgtcctc tgctgccagg
      721 gcgagtgcca gtggctgcac ctggacctca tcaaggagat gcggcagttc tgccagtccc
      781 tgttccccgt ggtggcctat gcctactgca ccatccccac ctaccccagc ggccagatcg
      841 gcttcatgct gtgcagcaag aacccgagca cgaacttcca ggagccggtg cagccgctga
      901 caca
//
