LOCUS       sequence-31471-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NSUN5
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aatattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggttc cgcgtggtag taggcctggt ccagcctccc agctgcctcg
      121 atttgtgcgt gtgaacactc tcaagacctg ctccgatgat gtagttgatt atttcaagag
      181 acaaggtttc tcctatcagg gtcgggcttc cagcctcgat gacttacgag ccctcaaggg
      241 gaagcatttt ctcctggacc ccttgatgcc ggagctgctg gtgtttcccg cccagacaga
      301 tctgcatgaa cacccactgt accgggccgg acacctcatt ctgcaggaca gggccagctg
      361 tctcccagcc atgctgctgg accccccgcc aggctcccat gtcatcgatg cctgtgccgc
      421 cccaggcaat aagaccagtc acttggctgc tcttctgaag aaccaaggga agatctttgc
      481 ctttgacctg gatgccaagc ggctggcatc catggccacg ctgctggccc gggctggcgt
      541 ctcttgctgt gaactggctg aggaggactt cctggcggtc tccccctcgg atccacgcta
      601 ccatgaggtc cactacatcc tgctggatcc ttcctgcagt ggctcgggta tgccgagcag
      661 acagctggag gagcccgggg caggcacacc tagcccggtg cgtctgcatg ccctggcagg
      721 gttccagcag cgagccctgt gccacgcact cactttccct tccctgcagc ggctcgtcta
      781 ctccacgtgc tccctctgcc aggaggagaa tgaagacgtg gtgcgagatg cgctgcagca
      841 gaacccgggc gccttcaggc tagctcccgc cctgcctgcc t
//
