LOCUS       sequence-31476-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FBL
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(612..623)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             812..829
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcgggaaga atgtgatggt ggagccgcat cggcatgagg
      121 gtgtcttcat ttgtcgagga aaggaagatg cactggtcac caagaacctg gtccctgggg
      181 aatcagttta tggagagaag agagtctcga tttcggaagg agatgacaaa attgagtacc
      241 gagcctggaa ccccttccgc tccaagctag cagcagcaat cctgggtggt gtggaccaga
      301 tccacatcaa accgggggct aaggttctct acctcggggc tgcctcgggc accacggtct
      361 cccatgtctc tgacatcgtt ggtccggatg gtctagtcta tgcagtcgag ttctcccacc
      421 gctctggccg tgacctcatt aacttggcca agaagaggac caacatcatt cctgtgatcg
      481 aggatgctcg acacccacac aaataccgca tgctcatcgc aatggtggat gtgatctttg
      541 ctgatgtggc ccagccagac cagacccgga ttgtggccct gaatgcccac accttcctgc
      601 gtaatggagg acactttgtg atttccatta aggccaactg cattgactcc acagcctcag
      661 ccgaggccgt gtttgcctcc gaagtgaaaa agatgcaaca ggagaacatg aagccgcagg
      721 agcagttgac ccttgagcca tatgaaagag accatgccgt ggtcgtggga gtgtacaggc
      781 catgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      841 tgctaacaaa gcccgaaagg aagctgantt ggctgctgcc accgctgagc aataacta
//
