LOCUS       sequence-314794-       13078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a concatemeric construct of a alpha-4:beta-2:alpha-5 
            (2:2:1) nAChR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13078)
  AUTHORS   Prevost MS, Bouchenaki H, Barilone N, Gielen M, Corringer PJ
  TITLE     Concatemers to re-investigate the role of alpha5 in alpha4beta2 
            nicotinic receptors.
  JOURNAL   Cell Mol Life Sci. 2020 May 29. pii: 10.1007/s00018-020-03558-z. 
            doi: 10.1007/s00018-020-03558-z.
  PUBMED    32472188
REFERENCE   2  (bases 1 to 13078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2020 May 29. pii: 10.1007/s00018-020-03558-z. doi: 
            10.1007/s00018-020-03558-z."
COMMENT     Sequence Label: pRK5-alpha-5last
FEATURES             Location/Qualifiers
     source          1..13078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      955..964
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9083..9092
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    9096..9230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9133..9152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9187..9206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        9299..9656
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9299..9490
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9322..9342)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9507..9642
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9676..9693)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9676..9692)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9685..9707)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9992..10011)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(10076..10267)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     10497..10518
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10672..10691)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10851..10869)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10937..11041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11042..11110,11111..11902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11260..11279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12073..12661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12562..12581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12815..12832
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12949..12970
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12985..12990,12991..13008,13009..13015)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13023..13039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13028..13050
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13047..13063
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13047..13063
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tgcagaagct cagaataaac gctcaacttt ggcagatcat cgatgccacc
      961 atggagctag ggggccccgg agcgccgcgg ctgctgccgc cgctgctgct gcttctgggg
     1021 accggcctcc tgcgcgccag cagccatgtg gagacccggg cccacgccga ggagcggctc
     1081 ctgaagaaac tcttctccgg ttacaacaag tggtcccgac ccgtggccaa catctcggac
     1141 gtggtcctcg tccgcttcgg cctgtccatc gctcagctca ttgacgtgga tgagaagaac
     1201 cagatgatga ccacgaacgt atgggtgaag caggagtggc acgactacaa gctgcgctgg
     1261 gacccagctg actatgagaa tgtcacctcc atccgcatcc cctccgagct catctggcgg
     1321 ccggacatcg tcctctacaa caatgctgac ggggacttcg cggtcaccca cctgaccaag
     1381 gcccacctgt tccatgacgg gcgggtgcag tggactcccc cggccattta caagagctcc
     1441 tgcagcatcg acgtcacctt cttccccttc gaccagcaga actgcaccat gaaattcggc
     1501 tcctggacct acgacaaggc caagatcgac ctggtgaaca tgcacagccg cgtggaccag
     1561 ctggacttct gggagagtgg cgagtgggtc atcgtggatg ccgtgggcac ctacaacacc
     1621 aggaagtacg agtgctgtgc cgagatctac ccggacatca cctatgcctt cgtcatccgg
     1681 cggctgccgc tcttctacac catcaacctc atcatcccct gcctgctcat ctcctgcctc
     1741 accgtgctgg tcttctacct gccctccgag tgtggcgaga agatcacgct gtgcatctcc
     1801 gtgctgctgt cgctcaccgt cttcctgctg ctcatcaccg agatcatccc gtccacctca
     1861 ctggtcatcc cactcatcgg cgagtacctg ctgttcacca tgatcttcgt caccctgtcc
     1921 atcgtcatca cggtcttcgt gctcaacgtg caccaccgct cgccacgcac gcacaccatg
     1981 cccacctggg tacgcagggt cttcctggac atcgtgccac gcctgctcct catgaagcgg
     2041 ccgtccgtgg tcaaggacaa ttgccggcgg ctcatcgagt ccatgcataa gatggccagt
     2101 gccccgcgct tctggcccga gccagaaggg gagccccctg ccacgagcgg cacccagagc
     2161 ctgcacccgc cctcaccgtc cttctgtgtc cccctggatg tgccggctga gcctgggcct
     2221 tcctgcaagt caccctccga ccagctccct cctcagcagc ccctggaagc tgagaaagcc
     2281 agcccccacc cctcgcctgg accctgccgc ccgccccacg gcacccaggc accagggctg
     2341 gccaaagcca ggtccctcag cgtccagcac atgtccagcc ctggcgaagc ggtggaaggc
     2401 ggcgtccggt gccggtctcg gagcatccag tactgtgttc cccgagacga tgccgccccc
     2461 gaggcagatg gccaggctgc cggcgccctg gcctctcgca acacccactc ggctgagctc
     2521 ccacccccag accagccctc tccgtgcaaa tgcacatgca agaaggagcc ctcttcggtg
     2581 tccccgagcg ccacggtcaa gacccgcagc accaaagcgc cgcccccgca cctgcccctg
     2641 tcgccggccc tgacccgggc ggtggagggc gtccagtaca ttgcagacca cctgaaggcc
     2701 gaagacacag acttctcggt gaaggaggac tggaagtacg tggccatggt catcgaccgc
     2761 atcttcctct ggatgttcat catcgtctgc ctgctgggga cggtgggcct cttcctgccg
     2821 ccctggctgg ctggcatgat ccagcaacag cagcaacaac agcaacagca gcaacagcag
     2881 caacaacagc aacagcagca aaccggtacg gatacagagg agcggctggt ggagcatctc
     2941 ctggatcctt cccgctacaa caagctcatc cgcccagcca ccaatggctc tgagctggtg
     3001 acagtacagc ttatggtgtc actggcccag ctcatcagtg tgcatgagcg ggagcagatc
     3061 atgaccacca atgtctggct gacccaggag tgggaagatt atcgcctcac ctggaagcct
     3121 gaagagtttg acaacatgaa gaaagttcgg ctcccttcca aacacatctg gctcccagat
     3181 gtggtcctgt acaacaatgc tgacggcatg tacgaggtgt ccttctattc caatgccgtg
     3241 gtctcctatg atggcagcat cttctggctg ccgcctgcca tctacaagag cgcatgcaag
     3301 attgaagtaa agcacttccc atttgaccag cagaactgca ccatgaagtt ccgttcgtgg
     3361 acctacgacc gcacagagat cgacttggtg ctgaagagtg aggtggccag cctggacgac
     3421 ttcacaccta gtggtgagtg ggacatcgtg gcgctgccgg gccggcgcaa cgagaacccc
     3481 gacgactcta cgtacgtgga catcacgtat gacttcatca ttcgccgcaa gccgctcttc
     3541 tacaccatca acctcatcat cccctgtgtg ctcatcacct cgctggccat ccttgtcttc
     3601 tacctgccat ccgactgtgg cgagaagatg acgttgtgca tctcagtgct gctggcgctc
     3661 acggtcttcc tgctgctcat ctccaagatc gtgcctccca cctccctcga cgtgccgctc
     3721 gtcggcaagt acctcatgtt caccatggtg cttgtcacct tctccatcgt caccagcgtg
     3781 tgcgtgctca acgtgcacca ccgctcgccc accacgcaca ccatggcgcc ctgggtgaag
     3841 gtcgtcttcc tggagaagct gcccgcgctg ctcttcatgc agcagccacg ccatcattgc
     3901 gcccgtcagc gcctgcgcct gcggcgacgc cagcgtgagc gcgagggcgc tggagccctc
     3961 ttcttccgcg aagccccagg ggccgactcc tgcacgtgct tcgtcaaccg cgcgtcggtg
     4021 caggggttgg ccggggcctt cggggctgag cctgcaccag tggcgggccc cgggcgctca
     4081 ggggagccgt gtggctgtgg cctccgggag gcggtggacg gcgtgcgctt catcgcagac
     4141 cacatgcgga gcgaggacga tgaccagagc gtgagtgagg actggaagta cgtcgccatg
     4201 gtgatcgacc gcctcttcct ctggatcttt gtctttgtct gtgtctttgg caccatcggc
     4261 atgttcctgc agcctctctt ccagaactac accaccacca ccttcctcca ctcagaccac
     4321 tcagccccca gctccaagca gcaacagcag caacaacagc aacagcagca acagcagcaa
     4381 caagtcgacc atgtggagac ccgggcccac gccgaggagc ggctcctgaa gaaactcttc
     4441 tccggttaca acaagtggtc ccgacccgtg gccaacatct cggacgtggt cctcgtccgc
     4501 ttcggcctgt ccatcgctca gctcattgac gtggatgaga agaaccagat gatgaccacg
     4561 aacgtatggg tgaagcagga gtggcacgac tacaagctgc gctgggaccc agctgactat
     4621 gagaatgtca cctccatccg catcccctcc gagctcatct ggcggccgga catcgtcctc
     4681 tacaacaatg ctgacgggga cttcgcggtc acccacctga ccaaggccca cctgttccat
     4741 gacgggcggg tgcagtggac tcccccggcc atttacaaga gctcctgcag catcgacgtc
     4801 accttcttcc ccttcgacca gcagaactgc accatgaaat tcggctcctg gacctacgac
     4861 aaggccaaga tcgacctggt gaacatgcac agccgcgtgg accagctgga cttctgggag
     4921 agtggcgagt gggtcatcgt ggatgccgtg ggcacctaca acaccaggaa gtacgagtgc
     4981 tgtgccgaga tctacccgga catcacctat gccttcgtca tccggcggct gccgctcttc
     5041 tacaccatca acctcatcat cccctgcctg ctcatctcct gcctcaccgt gctggtcttc
     5101 tacctgccct ccgagtgtgg cgagaagatc acgctgtgca tctccgtgct gctgtcgctc
     5161 accgtcttcc tgctgctcat caccgagatc atcccgtcca cctcactggt catcccactc
     5221 atcggcgagt acctgctgtt caccatgatc ttcgtcaccc tgtccatcgt catcacggtc
     5281 ttcgtgctca acgtgcacca ccgctcgcca cgcacgcaca ccatgcccac ctgggtacgc
     5341 agggtcttcc tggacatcgt gccacgcctg ctcctcatga agcggccgtc cgtggtcaag
     5401 gacaattgcc ggcggctcat cgagtccatg cataagatgg ccagtgcccc gcgcttctgg
     5461 cccgagccag aaggggagcc ccctgccacg agcggcaccc agagcctgca cccgccctca
     5521 ccgtccttct gtgtccccct ggatgtgccg gctgagcctg ggccttcctg caagtcaccc
     5581 tccgaccagc tccctcctca gcagcccctg gaagctgaga aagccagccc ccacccctcg
     5641 cctggaccct gccgcccgcc ccacggcacc caggcaccag ggctggccaa agccaggtcc
     5701 ctcagcgtcc agcacatgtc cagccctggc gaagcggtgg aaggcggcgt ccggtgccgg
     5761 tctcggagca tccagtactg tgttccccga gacgatgccg cccccgaggc agatggccag
     5821 gctgccggcg ccctggcctc tcgcaacacc cactcggctg agctcccacc cccagaccag
     5881 ccctctccgt gcaaatgcac atgcaagaag gagccctctt cggtgtcccc gagcgccacg
     5941 gtcaagaccc gcagcaccaa agcgccgccc ccgcacctgc ccctgtcgcc ggccctgacc
     6001 cgggcggtgg agggcgtcca gtacattgca gaccacctga aggccgaaga cacagacttc
     6061 tcggtgaagg aggactggaa gtacgtggcc atggtcatcg accgcatctt cctctggatg
     6121 ttcatcatcg tctgcctgct ggggacggtg ggcctcttcc tgccgccctg gctggctggc
     6181 atgatccagc aacagcagca acaacagcaa cagcagcaac agcagcaaca acagcaacag
     6241 cagcaagcta gcacggatac agaggagcgg ctggtggagc atctcctgga tccttcccgc
     6301 tacaacaagc tcatccgccc agccaccaat ggctctgagc tggtgacagt acagcttatg
     6361 gtgtcactgg cccagctcat cagtgtgcat gagcgggagc agatcatgac caccaatgtc
     6421 tggctgaccc aggagtggga agattatcgc ctcacctgga agcctgaaga gtttgacaac
     6481 atgaagaaag ttcggctccc ttccaaacac atctggctcc cagatgtggt cctgtacaac
     6541 aatgctgacg gcatgtacga ggtgtccttc tattccaatg ccgtggtctc ctatgatggc
     6601 agcatcttct ggctgccgcc tgccatctac aagagcgcat gcaagattga agtaaagcac
     6661 ttcccatttg accagcagaa ctgcaccatg aagttccgtt cgtggaccta cgaccgcaca
     6721 gagatcgact tggtgctgaa gagtgaggtg gccagcctgg acgacttcac acctagtggt
     6781 gagtgggaca tcgtggcgct gccgggccgg cgcaacgaga accccgacga ctctacgtac
     6841 gtggacatca cgtatgactt catcattcgc cgcaagccgc tcttctacac catcaacctc
     6901 atcatcccct gtgtgctcat cacctcgctg gccatccttg tcttctacct gccatccgac
     6961 tgtggcgaga agatgacgtt gtgcatctca gtgctgctgg cgctcacggt cttcctgctg
     7021 ctcatctcca agatcgtgcc tcccacctcc ctcgacgtgc cgctcgtcgg caagtacctc
     7081 atgttcacca tggtgcttgt caccttctcc atcgtcacca gcgtgtgcgt gctcaacgtg
     7141 caccaccgct cgcccaccac gcacaccatg gcgccctggg tgaaggtcgt cttcctggag
     7201 aagctgcccg cgctgctctt catgcagcag ccacgccatc attgcgcccg tcagcgcctg
     7261 cgcctgcggc gacgccagcg tgagcgcgag ggcgctggag ccctcttctt ccgcgaagcc
     7321 ccaggggccg actcctgcac gtgcttcgtc aaccgcgcgt cggtgcaggg gttggccggg
     7381 gccttcgggg ctgagcctgc accagtggcg ggccccgggc gctcagggga gccgtgtggc
     7441 tgtggcctcc gggaggcggt ggacggcgtg cgcttcatcg cagaccacat gcggagcgag
     7501 gacgatgacc agagcgtgag tgaggactgg aagtacgtcg ccatggtgat cgaccgcctc
     7561 ttcctctgga tctttgtctt tgtctgtgtc tttggcacca tcggcatgtt cctgcagcct
     7621 ctcttccaga actacaccac caccaccttc ctccactcag accactcagc ccccagctcc
     7681 aagcagcaac agcagcaaca acagcaacag cagcaacagc agcaacaact cgagcgctgc
     7741 ggtctagcgg gcgcggcggg cggcgcgcag agaggattat ctgaaccttc ttctattgca
     7801 aaacatgaag atagtttgct taaggattta tttcaagact acgaaagatg ggttcgtcct
     7861 gtggaacacc tgaatgacaa aataaaaata aaatttggac ttgcaatatc tcaattggtg
     7921 gatgtggatg agaaaaatca gttaatgaca acaaacgtct ggttgaaaca ggaatggata
     7981 gatgtaaaat taagatggaa ccctgatgac tatggtggaa taaaagttat acgtgttcct
     8041 tcagactctg tctggacacc agacatcgtt ttgtttgata atgcagatgg acgttttgaa
     8101 gggaccagta cgaaaacagt catcaggtac aatggcactg tcacctggac tccaccggca
     8161 aactacaaaa gttcctgtac catagatgtc acgtttttcc catttgacct tcagaactgt
     8221 tccatgaaat ttggttcttg gacttatgat ggatcacagg ttgatataat tctagaggac
     8281 caagatgtag acaagagaga tttttttgat aatggagaat gggagattgt gagtgcaaca
     8341 gggagcaaag gaaacagaac cgacagctgt tgctggtatc cgtatgtcac ttactcattt
     8401 gtaatcaagc gcctgcctct cttttatacc ttgttcctta taataccctg tattgggctc
     8461 tcatttttaa ctgtacttgt cttctatctt ccttcaaatg aaggtgaaaa gatttgtctc
     8521 tgcacttcag tacttgtgtc tttgactgtc ttccttctgg ttattgaaga gatcatacca
     8581 tcatcttcaa aagtcatacc tctaattgga gagtatctgg tatttaccat gatttttgtg
     8641 acactgtcaa ttatggtaac cgtcttcgct atcaacattc atcatcgttc ttcctcaaca
     8701 cataatgcca tggcgccttt ggtccgcaag atatttcttc acacgcttcc caaactgctt
     8761 tgcatgagaa gtcatgtaga caggtacttc actcagaaag aggaaactga gagtggtagt
     8821 ggaccaaaat cttctagaaa cacattggaa gctgcgctcg attctattcg ctacattaca
     8881 agacacatca tgaaggaaaa tgatgtccgt gaggttgttg aagattggaa attcatagcc
     8941 caggttcttg atcggatgtt tctgtggact tttcttttcg tttcaattgt tggatctctt
     9001 gggctttttg ttcctgttat ttataaatgg gcaaatatat taataccagt tcatattgga
     9061 aatgcaaata agtagaagct tggccgccat ggcccaactt gtttattgca gcttataatg
     9121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gggaattaat
     9241 tcggcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct
     9301 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     9361 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     9421 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9481 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9541 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9601 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     9661 tgttaacagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     9721 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     9781 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     9841 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     9901 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9961 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    10021 gccacgttcg ccggccttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
    10081 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
    10141 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
    10201 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
    10261 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
    10321 aacggccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
    10381 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
    10441 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    10501 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
    10561 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    10621 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
    10681 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
    10741 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
    10801 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
    10861 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
    10921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    10981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    11041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    11101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    11161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    11221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    11281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    11341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    11401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    11461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    11521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    11581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    11641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    11701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    11761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    11821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    11881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    11941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    12001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    12061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    12121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    12181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    12241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    12301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    12361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    12421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    12481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    12541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    12601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    12661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    12721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    12781 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    12841 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    12901 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    12961 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    13021 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaattaa
//
