LOCUS       sequence-314853-        1695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast display plasmid for expression of C-terminal fusions. 
            Modification of pJYDC1 vector with ALFA tag in MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Zahradnik J, Dey D, Marciano S, Schreiber G
  TITLE     An enhanced yeast display platform demonstrates the binding 
            plasticity under various selection pressures
  JOURNAL   bioRxiv Preprint 2020
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            Preprint 2020"
COMMENT     Sequence Label: pJYDC2
FEATURES             Location/Qualifiers
     source          1..1695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        178..619
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    178..295
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     590..613
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1389..1400
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1401..1427
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1561..1599
                     /codon_start=1
                     /product="rationally designed epitope tag that forms a 
                     stable alpha-helix (Gotzke et al., 2019)"
                     /label=ALFA-tag
                     /note="It may be beneficial to flank the ALFA-tag sequence 
                     with proline residues to preserve the helical structure."
                     /translation="SRLEEELRRRLTE"
ORIGIN
        1 cagcaacgcg gcactttgta actgagctgt cattttgctg gcccagctgg tcgaccgcgg
       61 ctaagcagta ctggtaccgt tgtggaaatg taaagagccc cattatctta gcctaaaaaa
      121 accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat tgaagtacgg
      181 attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc gtgcgtcctc
      241 gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa
      301 taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc agtaacctgg
      361 ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata atgcgattag
      421 ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg atctattaac
      481 agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac attttcggtt
      541 tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta atatacctct
      601 atactttaac gtcaaggaga aaaaaccccg gatcgaattc cctacttcat acattttcaa
      661 ttaagatgag gttcccatct attttcaccg ctgttgtttt tgctgcttct tctgctttgg
      721 ctgctccagc taatggtatg ttagaaaaat ttgttggcac ctggaagatc gaatcctctg
      781 aaaattttgg tgaatacttg aaggctatcg gtgccccaaa agaattggct gatgctggtg
      841 atgctactac tccagtcttg tacatttctc aaaaggatgg tgataagatg accgtcaaga
      901 ttgaaaacgg tccaccaact tttttggata cccaagtttc tttcaagttg ggtgaagaat
      961 tcgacgaatt tccatccgat agaagaaagg gtgttaagtc cgttgttaac ttgtctggtg
     1021 agaagttggt ttacgttcaa aagtgggatg gtaaagaaac cacttacgtc agagaaatca
     1081 aggacggtaa attggttgtt actttgacca tgggtgatgt tgttgctgtt agatcttata
     1141 gaagggcctc tgaagtttct gcacaggaac tgacaactat atgcgagcaa atcccctcac
     1201 caactttaga atcgacgccg tactctttgt caacgactac tattttggcc aacgggaagg
     1261 caatgcaagg agtttttgaa tattacaaat cagtaacgtt tgtcagtaat tgcggttctc
     1321 acccctcaac aactagcaaa ggcagcccca taaacacaca gtatgttttt aaggacaata
     1381 gctcgacgat tgaaggtaga tacccatacg acgttccaga ctacgctctg caggctagtg
     1441 gtggaggagg ctctggtgga ggcggtagcg gaggcggagg gtcggctagc catatgcacg
     1501 atgaactata atgaagcatg cttaattaag gcgccttatc tcgagggggg cggatcccca
     1561 tccagattgg aagaagaatt gagaagaaga ttaaccgaat aatagagatc tgataacaac
     1621 agtgtagatg taacaaaatc gactttgttc ccactgtact tttagctcgt acaaaataca
     1681 atatactttt cattt
//
