LOCUS       sequence-314952-        3429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock Down SH3+ Collybistin isoforms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   George S, Bear J Jr, Taylor MJ, Kanamalla K, Fekete CD, Chiou TT, 
            Miralles CP, Papadopoulos T, De Blas AL
  TITLE     Collybistin SH3-protein isoforms are expressed in the rat brain 
            promoting gephyrin and GABA-A receptor clustering at GABAergic 
            synapses.
  JOURNAL   J Neurochem. 2021 May;157(4):1032-1051. doi: 10.1111/jnc.15270. Epub
            2021 Jan 5.
  PUBMED    33316079
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/jnc.15270";
            journalName: "J Neurochem"; date: "2021-05"; volume: "157"; issue: 
            "4"; pages: "1032-1051"
COMMENT     Sequence Label: CBSH3+ shRNA-1
FEATURES             Location/Qualifiers
     source          1..3429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        171..483
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     402..422
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     polyA_signal    551..685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(575..594)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     629..648
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(783..803)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(783..801)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(822..838)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(822..838)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(835..857)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    846..862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(870..876,877..894,895..900))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    915..936
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1053..1070)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1224..1812)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1304..1323)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1983..2774,2775..2843))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2606..2625
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2844..2948)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2974..3429)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3111..3132)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3323..3342
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gtcgacagcc tgcaggaatg gccggccatc ttaattaaga aatttaaata
      121 tgtttaaaca tagggcccaa tggcgcgcca aggccatcta ggccaagctt atccgacgcc
      181 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc
      241 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
      301 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
      361 ttctataaga gatacaaata ctaaattatt attttaaaaa acagcacaaa aggaaactca
      421 ccctaactgt aaagtaattg tgtgttttga gactataaat atcccttgga gaaaagcctt
      481 gtttgggatg cttccaacaa ggattgcgaa caatccttgt tggaagcatc ctttttctag
      541 ataatacgta gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
      601 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      661 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
      721 tcagggggag gtgtgggagg ttttttaatt cttgcggccg cggcggtacc cagcttttgt
      781 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
      841 tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa
      901 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      961 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1021 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1081 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1141 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1201 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1261 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1321 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1381 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1441 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1501 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1561 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1621 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1681 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1741 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1801 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1861 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1921 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1981 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2041 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2101 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2161 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2221 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2281 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2341 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2401 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2461 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2521 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2581 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2641 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2701 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2761 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2821 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2881 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2941 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     3001 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3061 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3121 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3181 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     3241 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3301 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3361 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3421 agggcgcgt
//
