LOCUS       sequence-31521-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PKM2
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggttc cgcgtggtag taagccccat agtgaagccg
      121 ggactgcctt cattcagacc cagcagctgc acgcagccat ggctgacaca ttcctggagc
      181 acatgtgccg cctggacatt gattcaccac ccatcacagc ccggaacact ggcatcatct
      241 gtaccattgg cccagcttcc cgatcagtgg agacgttgaa ggagatgatt aagtctggaa
      301 tgaatgtggc tcgtctgaac ttctctcatg gaactcatga gtaccatgcg gagaccatca
      361 agaatgtgcg cacagccacg gaaagctttg cttctgaccc catcctctac cggcccgttg
      421 ctgtggctct agacactaaa ggacctgaga tccgaactgg gctcatcaag ggcagcggca
      481 ctgcagaggt ggagctgaag aagggagcca ctctcaaaat cacgctggat aacgcctaca
      541 tggaaaagtg tgacgagaac atcctgtggc tggactacaa gaacatctgc aaggtggtgg
      601 aagtgggcag caagatctac gtggatgatg ggcttatttc tctccaggtg aagcagaaag
      661 gtgccgactt cctggtgacg gaggtggaaa atggtggctc cttgggcagc aagaagggtg
      721 tgaaccttcc tggggctgct gtggacttgc ctgctgtgtc ggagaaggac atccaggatc
      781 tgaagtttgg ggtcgagcag gatgttgata tggtgtttgc gtcattcatc cgcaaggcat
      841 ctgatgtcca tgaagttagg aaggtcct
//
