LOCUS       sequence-315263-        9534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of rat WT Myosin Va with deletion of the 1005 
            N-terminal residues.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Frank M, Citarella CG, Quinones GB, Bentley M
  TITLE     A Novel Labeling Strategy Reveals That Myosin Va and Myosin Vb Bind 
            the Same Dendritically Polarized Vesicle Population.
  JOURNAL   Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.
  PUBMED    32959500
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2020 Sep 21. doi: 10.1111/tra.12764."
COMMENT     Sequence Label: pBa.Halo-myosin Va tail 1006-1828
FEATURES             Location/Qualifiers
     source          1..9534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        418..693
                     /label=chicken beta-actin promoter
     primer_bind     1626..1648
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1740..1760
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             1776..2660
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     polyA_signal    5371..5505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5408..5427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5462..5481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5537..5553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5537..5553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5550..5572)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5561..5577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5585..5591,5592..5609,5610..5615))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5630..5651
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5768..5785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5939..6527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6019..6038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6698..7489,7490..7558))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7321..7340
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7559..7663)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7731..7749
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7787..7809)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7909..7928
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8122..8144
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8136..8153
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8137..8153
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct cattgtatca
       61 cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac gcacactcat
      121 tgtatcacgt cagctcagtg gctgcacgca caccggtgtt attgctgctc ggtgcgtgca
      181 tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg ctatccctgc
      241 ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc acctcggggg
      301 tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca ctgtggaatc
      361 aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac cctcacctcg
      421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
      541 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
      601 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      661 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
      721 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      781 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
      841 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
      901 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
      961 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1021 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     1081 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1141 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     1201 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     1261 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     1321 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1381 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     1441 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     1501 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     1561 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
     1621 ggcggggttc ggcttctggc gtgtgaccgg cggggtttat atcttccctt ctctgttcct
     1681 ccgcagcccc caagcttggc gcgcctaggc ctaccatggc tagcctcgag ccaaccactg
     1741 aggatctgta ctttcagagc gataacgatg gatccgaaat cggtactggc tttccattcg
     1801 acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg
     1861 atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca
     1921 tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca
     1981 aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct
     2041 tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc
     2101 tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt
     2161 tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag accttccagg
     2221 ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg
     2281 gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac cattaccgcg
     2341 agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa
     2401 tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac tggctgcacc
     2461 agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc ccaccggccg
     2521 aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccgggtc
     2581 tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg cgctggctgt
     2641 ctactctgga gatttccggt ctgtacggaa tgcagaagct gatctcagag gaggacctgc
     2701 aaaaacttat ttctgaagag gatctgcaaa agcttatttc tgaagaagat ctgtacaagg
     2761 gaggtggctc tagcaaaaag tccattgaag aacgagcaga taaatacaaa caagaaactg
     2821 agcagctggt gtcaaacttg aaggaagaaa acactctgct gaagcaggaa aaggagaccc
     2881 tcaatcacct catggtggag caggctaagg agatgacaga aactatggag aggaagttag
     2941 tagaagaaac aaaacaactg gagctcgacc tgaatgatga gaggctgagg tatcagaacc
     3001 tcctgaatga gttcagtcgt ctggaggagc gctatgacga cctcaaggaa gagatgaccc
     3061 tgatgctgaa tgtgcctaag ccaggacaca agagaacaga ctctacccac agcagcaatg
     3121 agtctgaata caccttcagc tcagaatttg cagaaactga agacattgca ccaaggacag
     3181 aggagccaac tgagaagaag gtgcctttgg atatgtcatt gttccttaag ctccagaagc
     3241 gtgttacaga gctgggacag gagaagcagt tgatgcagga tgagctggac cgaaaggagg
     3301 agcaggtgtt gcgcagcaag gcaaagggag gagaaagacc acagattaga ggtgctgaac
     3361 tgggatatga gtccctcaag cgtcaggaac tggagtcaga aaacaaaaag ctgaagaatg
     3421 agctgaatga gctacgcaaa gccctgagtg agaagagtgc ccccgaagtg aatgcgccag
     3481 gcgcgcccgc ttaccgcgtc ctcatggagc agctgacggc cgtgagcgag gagctcgatg
     3541 tccgcaagga ggaagtcctc attttaaggt ctcagctggt gagccagaaa gaagccatcc
     3601 aacccaagga tgacaagaat acaatgacag attccacaat acttttggaa gatgtacaga
     3661 aaatgaaaga caaaggtgaa atagcacaag catatattgg tttgaaagaa acgaacaggc
     3721 tcttagaatc ccagctacag tcgcagaaga ggagccatga aaatgaggct gaggccctcc
     3781 gtggggagat ccagagccta aaggaagaaa acaaccggca acagcagctg ctggcccaga
     3841 acctgcagct gccccctgag gcccgcattg aggccagcct gcagcatgag atcacccggc
     3901 tgaccaatga aaacctggat ctgatggaac aacttgaaaa gcaggataaa accgtccgga
     3961 aactgaagaa acaactaaaa gtctttgcca aaaaaatcgg tgaactagaa gtggggcaga
     4021 tggagaacat atccccagga cagatcatcg atgagcctat ccggccggtc aacattcccc
     4081 ggaaaggaaa ggatttccaa gggatgctgg agtacaagcg ggaggacgaa cagaagcttg
     4141 ttaagaacct gattctagaa ctaaagccac gtggtgtggc tgtcaatctg atttcagggt
     4201 taccggcata tatcttgttt atgtgtgtgc gacatgccga ctacctggac gatgatcaga
     4261 aagtaagatc attgctgaca tcaacaatta acagcatcaa aaaagtcctg aagaaaagag
     4321 gtgacgattt tgaaactgtc tccttctggc tctctaacac atgtcgattt ttgcactgtt
     4381 tgaagcaata tagtggagaa gagggcttca tgaaacacaa cacgtctcgc cagaatgaac
     4441 actgcctcac caattttgac cttgctgagt atcggcaagt actgagtgac ttggccattc
     4501 agatctatca gcagcttgtg agggtgttag agaacattct tcagccaatg atagtctcag
     4561 gcatgctaga gcatgaaaca attcagggag tatctggggt gaagcccaca ggactcagaa
     4621 agcgaacctc cagtatcgcc gatgagggca cctacacact ggactccatc ctgcggcagc
     4681 tcaactcctt ccattccgtc atgtgtcagc atggcatgga cccagagcta atcaagcagg
     4741 tggtcaagca gatgttctac atcgtgggcg ccatcaccct gaacaacctc ctgctgcgca
     4801 aggacatgtg ctcctggagc aaaggcatgc agatacggta caatgtcagt caattggaag
     4861 aatggctacg tgacaagaat ctaatgaaca gtggggcaaa agagactctg gaacctctta
     4921 tccaggctgc tcagcttttg caagtgaaaa agaaaactga cgatgacgcg gaagccatct
     4981 gctccatgtg caacgctctg accacagccc agatcgtcaa agtgttgaat ctgtacacac
     5041 ccgttaatga gtttgaggaa agggtctctg tttcatttat ccgcactata caggtgcggt
     5101 tacgagacag gaaagactct ccacagctgc tcatggatgc aaaacacatc tttcctgtca
     5161 ctttcccctt taacccatcc tccctggccc tagaaaccat ccagattcca gccagcctgg
     5221 gcctgggctt cattgcaagg gtctgaaggg ctaccgaatt cattgatcat aatcagccat
     5281 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     5341 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     5401 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5461 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccccgg gtaccgagct
     5521 cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5581 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5641 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5701 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5761 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5821 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5881 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6061 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6121 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6181 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6241 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6301 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6361 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6421 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6481 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6541 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6601 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6661 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6721 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6781 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6841 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6901 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6961 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7021 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7081 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7141 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7201 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7261 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7321 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7381 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7441 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7501 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7561 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7621 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7681 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     7741 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     7801 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     7861 agggcgcgtc agcgggtgtt ggcgggtgtc ggggttggct taactatgcg gcatcagagc
     7921 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     7981 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     8041 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     8101 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagt
     8161 tgggatcttt gcattggccc acggctctca ggatggggat gctccccttc agcacccggt
     8221 tccccttgga aactgatggt cctggctctg tggcatggca gtggcactgt gaggagcccc
     8281 taccagcagc acacagtggg tttggcactg ccacgctccg gatgccgcgc tctgatccaa
     8341 ccccataatc aagggaaccc gaattgcccc atcattgccc ccaccacccc catcctgccg
     8401 ggccctcaca ccccacgctg ccttgtggtg acattcccca gcccaaaccc acggcttcat
     8461 ggctaccgcg gggcatttcc cattgccgcc ccattatcag ctctgcacac ctcccgctgt
     8521 acccatgcct cgtggctgcc cttctttgac gtataatctt ctaattaata cccggccttg
     8581 tcaaagtgga gcacaaacgt taattaattc cccagcaggc aggtaattaa cagtgtgact
     8641 ccctttttgc tgcgagtggg gctgatacag agagatgtgg cactatggag cccacggggt
     8701 cctggcactg ggtgcccacg gaggtcccca tgtgctgcag tgtcaccgcc tccgaggtga
     8761 cagtattgtc cctgcggtgt ccctgcagct cagctctgtc cacagggcca cctccagttt
     8821 ggaggggaca caatgcagcc ccgatgcaac ccatcctcgc agcatcccag ggacaaagac
     8881 cccactgcaa gaccgcacac agggctgggt cccgctcccc taatatctac agtgcttttg
     8941 catggcccct taatcaatgc agttaatcag catgcgctca tgcaccgctc tggagctgca
     9001 aagcccctcg cagcgctgct caccaacacc gcgcaccgcc ccggcccagc ctgcagcacg
     9061 cgctgcaaac aggaaagaaa caaaatattg cccaaatgta ggcaaaggca ttcggctgcc
     9121 ttgacctccg ccgggccggg ccctgcctga ctcagctcct tactcagcgc tcgcttcctc
     9181 cctccggctg ccaccgccgc agcgcacacc ctgacaaaga gtggccctta acgggctctg
     9241 aggtgcaccc agcagtgcac tcagcagtcc aagggccggc ctggaggttt gcaccgctac
     9301 gtgctgacat tagcattgaa cttggccctg ggtagtgctg caggccgggc ggggtgggtg
     9361 tagagagtgc agcgcgcgtt gcacccggtg ccccttcccc tcccttgcat cccagcaggc
     9421 tgcaccccag caccaggccc gtgcatgcat gctcctggtg ttattgcagc ctggtgcatg
     9481 catgcgtctt agtggtgcag cgctgtgcat gcatcctcct tggtgtgtag cagc
//
