LOCUS       sequence-315285-        4259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression in CHO cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Orlova NA, Kovnir SV, Hodak JA, Vorobiev II, Gabibov AG, Skryabin KG
  TITLE     Improved elongation factor-1 alpha-based vectors for stable 
            high-level expression of heterologous proteins in Chinese hamster 
            ovary cells.
  JOURNAL   BMC Biotechnol. 2014 Jun 14;14:56. doi: 10.1186/1472-6750-14-56.
  PUBMED    24929670
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-06-14"; volume: "14:56. doi"; pages: " 
            10.1186/1472-6750-14-56"
COMMENT     Sequence Label: p1.1
FEATURES             Location/Qualifiers
     source          1..4259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctagcatatt acccctaaca cctgccaccc cagtcttaat cagtggtgga agaacggtct
       61 cagaactgtt tgtctcaatt ggccatttaa gtttaatagt gaaagactgg ttaatgataa
      121 caatgcatcg gaaaaccttc aggaggaaag gagaatgttt tgtggaacat ttttgtgtgt
      181 gtggcagttt taagttatta gttttcaaaa tcagtacttt ttaatggaaa caacttgacc
      241 aaaaatctgt cacagaattt tgagacccat taaaatacaa gtttaatgag aagtctgtct
      301 ctgttaatgc tgaagtcatt actaagtgct tagcttagca aggtatgtgg atgcccattt
      361 gtgttccaag ggattggact gttcatcagg acccagagct gagtttcaag ggctcaagag
      421 atggcttatt acctgtgggt gtcttgaagg ttctggttgg gacaaattag gaatgttttt
      481 ggcagacatg gtgactaccc ttcatctggg tgagttcagt tgatttgtct tgagcctttg
      541 gggtttacac aagtaaatga catcatacag ttagtgtatt gttagtgaat attaatatat
      601 gaggcaggct ttgctctagc aattttagaa ctagttttca ggaaagggtt catcttgtgc
      661 attggatgtt tgattctatc acttagagtt taaactgaaa gtgctcaaga ggttttattt
      721 aggctgggat ataaataagc ctttctgtag cttgtaatgg tatcaggaat ttaaaaggcc
      781 atctggggca caaagattaa gcagaaaagg tagaaggtga gattggggga ctttgagtac
      841 ttcacacact ttaatgtgtg agtgctttag tgcatatagt acaactgcca gataagggca
      901 tccacatctg attgtttgga aggcaccttg tggtttctgg gaattcagaa ttgggagaaa
      961 aagtgctccc aaccgctgaa gcccttggta atctgcaggg tgtttattta gcaggagata
     1021 aggacaaaaa gttatagtgt ggagttggtt gagttggtag atgtcattac aacaggtggt
     1081 cttaaattgg gttaggagtc actttgaaat acctgggcca taagcaaagt ggcattttca
     1141 cctttcagga gaaactggta cacttatcca ttctatagtg catgcttgtt caattgggct
     1201 gatgactaaa ccggtgacta aaggtttgtc agtataaatg gatgggttgt aggcagacgg
     1261 tgaggaatta ctatacctgc aaggagtcat tgcctgatct gcctggaaag gggcaggatt
     1321 gagtctcaga aggtgtacac cataggatat ggaaaaattt gtcacgccta gcattcaact
     1381 tagtggtgta gcgccaccta ctggcacttt aaaagcttag catagaggag catgtgtgtt
     1441 aggagctcgg atgggatcca gggcctcaag gtttgcatgt aaataaaagc cctttaccaa
     1501 attaactaca taccagcata catcagtcct ttagtgttga aaaacagaag tgaaagctaa
     1561 tatatatagt gcttgcttta tttaagtcta gctgattacg tgtttggttg ccagtgtgac
     1621 tagtctggag ttgaatttgt cctcagacac gtaaaatgga atttgggatt cacaacactc
     1681 tagtatgagg gacctaatgg cctgtaccag gcacaaacgt gtctataaac tacacaaaac
     1741 gaaggaattt acaggaatta ggaaggtatt cttaacatta aaacattatg ggcattttaa
     1801 aaaaagcttt gacaggattt ctttgtcatg gctgtcctgg agctagttgt gtagaccagg
     1861 ctgggctgaa atcttgtctg cctgcctggc ttggacactt ttttattatg tatacaacat
     1921 tctgcttcca tgtatatctg cacattagaa gacggcacca gatctcctaa tggatggttg
     1981 tgagccacca tgtggttgct gggaattgaa ctcaggacct ctggaagagc agtgctctta
     2041 acctctgagc catctccagc cccagcttgg gcacattttt aatggctggg aaatcaaacc
     2101 ccctaggcct tctgtcagta atgaagggct tttggctacc gagagtagga tttaaggtta
     2161 ttcggagctg caggtctgcc tcagtgcagg tttgggagtc cagcatctta gaaaatgcag
     2221 tgaagccaag ctgagctata ttttgtttaa aaaaaaaata agtgggtaaa gtgctgctga
     2281 gcctgatgac caagctggga cacaagtaga agaacatagg ccaatgctct atattaaaag
     2341 catgggtcat ttttaatgct cttgagaagg ctatgcctac actactctca gccaccgcag
     2401 cgtgtttaaa ttaaactagt ttggaaattt tctttggggg taagctattt aacctagtgc
     2461 cttggcaggt atactactga actctcctcc tcattccttt ttgtttttta agaatttcag
     2521 tcaggctcag gcagccctta aacttgtgat taagcctgag aacagttacg attatgagcc
     2581 tattagtata ccgatcaata tgtgaatttt tttgggatgg gggtcaggcc tccctgcctc
     2641 ccaaatactg ggactaaagg ctgcaccacc acaacctggc tcttgaaata cttttctaca
     2701 ttttttgggg ggcatgggtg ggagagcagg gtttctctgt attagccctg gctctcctgg
     2761 aactctgtag accaggctat tcttgagctc agattagcct gtctctgcct cctaaattct
     2821 gggattaaag gtgtgtgcta ctgctgcctg gctacaaaga catttttttt tttcttaaat
     2881 ttaaaaacaa aagtggttct tttagaaggg tggttggtgt tggcacatac tccaagcact
     2941 caggttttga gtttgtccca ggaatgaaga ctgcattact gccgcccctc cctggtaagg
     3001 gctacacaga gaaatcctat ttggagcctg tcctggtaac tcgctctgta gaccaggctg
     3061 gcctcgaact caagagaacc acctgcctct gaatgctggt attaagggca ggcaccacca
     3121 acacccagcc taaaaaatgt ctttttttta aagatttttt tttttttttt tacagaataa
     3181 acattctgtt tacaatattc tgcttctatg tatatctgca cactagaaga gggcacccga
     3241 tctcataatg gatggttgtg agccaccaag tggttgctgg gaattgaact cagaacctct
     3301 ggaagagcag tcagtgctct taacctctga gccatctctc cagcccctaa aaatggctct
     3361 tgagataggg tctcaagtag tttgagactg agttggctat ataaacaagg ctggcacata
     3421 gcaccatgta cagctgggtt tagtttacat ggggtgtttt tgtctctgga ggcaggagga
     3481 tcatttgagc atagggagtt aatagtgagg tcatgtttta tctactcttc tgaattgaga
     3541 actaagctga tgcaaagcaa gtttgactga agaagtccag tttatgagaa caagggtgga
     3601 aactaatgtg tcaaagatgg ccttgcatgt gttttagatg atgacccagt cacttgggaa
     3661 ttactggatg tgtaagacct atatcttgac aggagtgaac agtgtcttat aggtcctata
     3721 tgaaagaaat gagacatacc cattttgttt cccctaagaa ttcacttttc ctaacctggt
     3781 tcatgctatt taggttattt tacttgcaaa tcctagggtg ctcccttacc cagtattgct
     3841 tatgtggcac caaagtcact cactcccatg atttgcaagt ctctgggaac ttccatgaca
     3901 acctagaata gcaactcaaa tacattttct cagtaccaat tttgaagaaa aaatattttg
     3961 caaaatagct gtatggatgg gtactaaata gtgaggttat ctccagaagg cctatgaaga
     4021 attaaggttg agttcagttg agttcagcag caagtttaag gttcatccat ttttgtacag
     4081 tgttttccta ttacggtaag tgttttgcct gcaggaatat ctgtaccaca tgcttgcctg
     4141 gtacctatat cggccagaag agggctttgg atcctctgga cttgaattac agatgggtat
     4201 tagccaccat ttaggtgctg ggaattgaaa ccaagtcctc tggaagaaca gcaagtgac
//
