LOCUS       sequence-31529-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SMAP1L
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             526..543
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(596..614)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      610..657
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(781..800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt atgacaggca agtcggtgaa
      121 ggacgtggat cggtaccagg ctgtcctggc caacctgctg ctggaggagg ataacaagtt
      181 ttgtgcagat tgccagtcta aagggccgcg atgggcctct tggaacattg gtgtgttcat
      241 ctgcattcga tgtgctggaa tccacaggaa tctgggggtg cacatatcca gggtaaagtc
      301 agttaacctc gaccagtgga ctcaagaaca gattcagtgc atgcaagaga tgggaaatgg
      361 aaaggcaaac cgactttatg aagcctatct tcctgagacc tttcggcgac ctcagataga
      421 cccagctgtt gaaggattta ttcgagacaa atatgagaag aagaaataca tggaccgaag
      481 tctggacatc aatgcctgat gacgaagctt gcggccgcac tcgagcacca ccaccaccac
      541 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      601 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      661 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      721 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      781 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      841 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      901 aacttgatta agggtgatgg ttcacgta
//
