LOCUS       sequence-31540-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Cp-YWHAZ
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..228
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     312..331
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        312..330
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    331..355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             370..392
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             411..428
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             441..461
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
       61 ngttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
      121 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
      181 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
      241 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
      301 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
      361 agaaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc
      421 atcatcacag cagcggcaga gaaaacttgt atttccaggg catcactgaa aagaatatga
      481 aattatcaga aggagcatat agagctaaat tggctgatat ggttggtaac tacaaggatg
      541 taattaaagt actcactgaa tcttctgact ttagagataa ctctcttata ttgttattgg
      601 ctggatccct aagaaacaga gttacttcga ttcgaaattc tctcaaatct attaagagcc
      661 aag
//
