LOCUS       sequence-315476-        5984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0518:Dendra
FEATURES             Location/Qualifiers
     source          1..5984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1928..1951
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2208..3008
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3133..3721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3622..3641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggcagaggt agagtttcgt gatgtcacac gcagctatcc
      121 cggtggggtt caggcgctca agggacttga tctcacggtg gccgacggcg agttcctgat
      181 cctggtcggg ccttccggct gcggcaagag cacggcgctg cgaatgctgg cgggcctcga
      241 caaaccgaca gcgggcgaga tccggatcgg cggtgccgtc gtcaaccatc tgtcacccgg
      301 gcagcgtgac atcgcgatgg tgttccagaa ctacgcgctg tacccacaca tgtcggtgta
      361 ccgcaacctg gcctatgggc tgcgccaaag gcgcactcca cgcgccgaaa tcgaccggcg
      421 cgtgcgggaa acggccgaac tgctgcagat caccgagttc ctcgaccgca agcccgggca
      481 actgtccggc ggacaacgtc aacgtgtagc catgggacgc gcgttggtgc gcgaaccgca
      541 ggcattcctg ctcgacgaac ccctgtcgaa cctcgacgcc aaactgcgca accaggttcg
      601 cggcgatctc aagcgtttgc accgagaggt tccggtcacg tcgatctacg tcacccacga
      661 ccaggtcgaa gccatgacgc tgggggaccg gttgtgcgtg atgtcgcagg gcgaggtgca
      721 gcagatcggc accaccgacg acatctacaa ccggcccgcc aacacgttcg tcgcggcgtt
      781 catgggcagt ccgcccatga acctgatgcc gggcatcgtg cggcagggtg tgctgcacgt
      841 cggcggggaa tcggtcacgc cggtgtcgtg tccggaaggc ccggtgaccg tgggtgcccg
      901 accggagcac ctcgaactga gcccggtctg gggcgatggc atggtgccgg cacgtgtcga
      961 tttcgtggag ccactcggca gccacagtct ggtgaccgcg ctgttggggg acgatcccgc
     1021 gagtacgacg cgagtgatcg tgcaggcgcc cgcggacacc gacctaccgt cgggcacgcg
     1081 catcggcctg acactgccgc cgcagcggac gtatttcttc gatgccgaga gcggagatgc
     1141 gctgaccgcc gcgagtatgg ggcgtgagcg catatctctc gcaaagcttg gtgcggccgc
     1201 gggcaacacg cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga
     1261 gggcaacgtg aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga
     1321 agggacccag accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga
     1381 tatcctcact accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat
     1441 ccctgactac ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt
     1501 cgaagacaag ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt
     1561 ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa
     1621 gaccctcaag tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg
     1681 caacatcaac atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac
     1741 cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat
     1801 cgaaatcctg gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc
     1861 acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta
     1921 tttccaggac tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa
     1981 acgggtcttg aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta
     2041 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     2101 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     2161 ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg
     2221 tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca
     2281 accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc
     2341 ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag
     2401 ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt
     2461 cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa
     2521 agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct
     2581 ctgatccatt gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac
     2641 tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg
     2701 ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg
     2761 gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg
     2821 cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg
     2881 ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc
     2941 cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt
     3001 cgattggctg agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga
     3061 ttgctggggc aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat
     3121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3181 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3241 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3301 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3361 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3421 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3481 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3541 gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3601 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3661 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3721 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3781 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3841 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg
     3901 atcctctaga gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag
     3961 ccagctcgct gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc
     4021 cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc
     4081 caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca
     4141 gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt
     4201 cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga
     4261 ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg
     4321 ccgaccacat atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag
     4381 tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca
     4441 acccggtagc cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg
     4501 ctgaagactc agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg
     4561 aggtactacg cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc
     4621 gagaagcggc tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca
     4681 gccgccagcg ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc
     4741 gcagacggca ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta
     4801 ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg
     4861 ggtaggaagc acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac
     4921 acagcggtcg aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc
     4981 gatgagcgcg acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc
     5041 ttcgagcact accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag
     5101 ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg
     5161 cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg
     5221 tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag
     5281 gaccgtacga agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac
     5341 cggctgtcga agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg
     5401 ccggaactcc gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt
     5461 gcgacgacca aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag
     5521 taccagatgg cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc
     5581 aagacctcct gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg
     5641 tttcttgtca gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg
     5701 cggagacctg ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagaaatct
     5761 agacggtgac cacaacgcgc ccgctttgat cggggacgtc gaaatattgg atcgtcggca
     5821 ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat gatcggccgc atccccacgg
     5881 tgctgcgcag tgagctctac gccatcccgg cgttgatctg tgcgttcgca cgcacaggcc
     5941 cggtgtgaga agggtctctg acgagcggga gaactcccta tcag
//
