LOCUS       sequence-315477-        5759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0622:Dendra
FEATURES             Location/Qualifiers
     source          1..5759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1703..1726
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1983..2783
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2908..3496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3397..3416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggggcctaa cgctgttgag ctgaccaccg accaggcctg
      121 gtgcctcgcc gatgtcctgg gcgccgggtc gtacccgtgg gtgctggcga tcacgccgcc
      181 gtacagcgat cactcgcagc ggtcggcgtt cctcgcagcg cagtccgcgg aactgacccg
      241 gatgggcgtc gtcaactctg ccggtgcggt cgatccccgg gtcgcgcagt ggatcacgac
      301 ggtctgccgt gcgacacagt ggctcgatct gcggttcgtg tcgggaccgg gtgatctgtt
      361 gcgcggcatg gtcgcccgcc ggtcggaaga aaccgtcgtc gcgttgcgca acgcgcagtt
      421 ggtcaccttc accgcgatgg acatcggcca ccagcacgca ctggtgccgg tgctcacagc
      481 ggggctgtca ggacggaaac ctgcccggtt cgacgacttc gcactgcccg ccgccgcggg
      541 tgcgcgggcc gacgagcaga tccgcaacgg agccccactg gccgaggtgc tggagttcct
      601 cggggttccg ccctcggcgc ggccactggt cgagtcggtg ttcgacggtc ggcgcaccta
      661 cgtcgagatc gtcgcgggcg agcatcgcga cggccaccgc gtcaccaccg aggtgggggt
      721 cagcatcatc gacaccccac acggccggat actggttcac ccgacgaaag ccttcgacgg
      781 agagtggatc tcgaccttca cgcccggcag tgccgacgcg atcgccatgg cggtcgagcg
      841 gctgaccgca tccctaccca gcggatcctg gttccccgac cagccgctca cccgcgactt
      901 cgacgaagac gcggcaaccc accgagaacc agttctccag agaagaaccc agaaagcaaa
      961 gcttggtgcg gccgcgggca acacgcctgg catcaatctg atcaaggagg acatgcgtgt
     1021 caaggtgcat atggagggca acgtgaacgg tcacgccttc gtgatcgagg gggagggtaa
     1081 gggtaagcct tacgaaggga cccagaccgc caacctcacc gtcaaggagg gcgcccctct
     1141 cccattctcg tacgatatcc tcactaccgc ggtgcattac gggaaccgcg tgttcaccaa
     1201 gtaccccgag gatatccctg actacttcaa gcagagcttc ccggagggat actcctggga
     1261 gcggacgatg accttcgaag acaagggtat ctgtacgatc cgcagcgaca tctcgctgga
     1321 aggggactgt ttcttccaga acgtccgttt caaggggacc aactttccgc cgaacggccc
     1381 ggtcatgcag aagaagaccc tcaagtggga gccgagcacg gagaagctgc acgttcggga
     1441 cggcctcctg gtcggcaaca tcaacatggc gctgctgctg gaaggcggcg gccactacct
     1501 atgcgacttc aagaccacgt acaaagcgaa gaaggtcgtc cagctgcccg acgcgcactt
     1561 cgtggaccac cgcatcgaaa tcctggggaa cgattcggac tacaacaagg tcaagttgta
     1621 cgagcacgct gtggcacgct atagcccgct cccgtcgcag gtctggttcg aaggcggcgg
     1681 cggggagaac ctgtatttcc aggactacaa ggacgacgac gacaagtgat ttaaataacc
     1741 ccttggggcc tctaaacggg tcttgagggg ttttttgtta actagcgtac gatcgaattc
     1801 ctgcagctca cggtaactga tgccgtattt gcagtaccag cgtacggccc acagaatgat
     1861 gtcacgctga aaatgccggc ctttgaatgg gttcatgtgc agctccatca gcaaaagggg
     1921 atgataagtt tatcaccacc gactatttgc aacagtgccg ttgatcgtgc tatgatcgac
     1981 tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca
     2041 tcggtcagct tctcaacctt ggggttaccc ccggcggtgt gctgctggtc cacagctcct
     2101 tccgtagcgt ccggcccctc gaagatgggc cacttggact gatcgaggcc ctgcgtgcta
     2161 cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt
     2221 tcgatcctgc cacgtcgccc gttacaccgg accttggagt tgtctctgac acattctggc
     2281 gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt tgcggcagcg gggccacagg
     2341 cagagcagat catctctgat ccattgcccc tgccacctta ctcgcctgca agcccggtcg
     2401 cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg cgtgggacac gatgccaaca
     2461 cgacgctgca tcttgccgag ttgatggcaa aggttcccta tggggtgccg agacactgca
     2521 ccattcttca ggatggcaag ttggtacgcg tcgattatct cgagaatgac cactgctgtg
     2581 agcgctttgc cttggcggac aggtggctca aggagaagag ccttcagaag gaaggtccag
     2641 tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat tgtggcgaca gccctgggtc
     2701 aactgggccg agatccgttg atcttcctgc atccgccaga ggcgggatgc gaagaatgcg
     2761 atgccgctcg ccagtcgatt ggctgagctc atgagcggag aacgagatga cgttggaggg
     2821 gcaaggtcgc gctgattgct ggggcaacac gggggatcca ctagttccat gagcgtcaga
     2881 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     3061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3121 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3181 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3241 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3301 atgagaaagc gccacgcttc ccgaggggag aaaggcggac aggtatccgg taagcggcag
     3361 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3421 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3481 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3541 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3601 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg
     3661 ccgcggtacc cggggatcct ctagagtcga ccaccaaggg caccatctct gcttgggcca
     3721 ccccgttggc cgcagccagc tcgctgagag ccgtgaacga cagggcgaac gccagcccgc
     3781 cgacggcgag ggttccgacc gctgcaactc ccggtgcaac cttgtcccgg tctattctct
     3841 tcactgcacc agctccaatc tggtgtgaat gcccctcgtc tgttcgcgca ggcggggggc
     3901 tctattcgtt tgtcagcatc gaaagtagcc agatcaggga tgcgttgcaa ccgcgtatgc
     3961 ccaggtcaga agagtcgcac aagagttgca gacccctgga aagaaaaatg gccagagggc
     4021 gaaaacaccc tctgaccagc ggagcgggcg acgggaatcg aacccgcgta gctagtttgg
     4081 aagaatgggt gtctgccgac cacatatggg ccggtcaaga taggttttta ccccctctcg
     4141 gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt
     4201 tgcacaggag ttgcaacccg gtagccttgt tcacgacgag aggagaccta gttggcacgt
     4261 cgcggatggg gatcgctgaa gactcagcgc agcgggagga tccaagcctc atacgtcaac
     4321 ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg acaacaagat ggacgccgaa
     4381 gcctggctcg cgggcgagaa gcggctcatc gagatggaga cctggacccc tccacaggac
     4441 cgggcgaaga aggcagccgc cagcgccatc acgctggagg agtacacccg gaagtggctc
     4501 gtggagcgcg acctcgcaga cggcaccagg gatctgtaca gcgggcacgc ggagcgccgc
     4561 atctacccgg tgctaggtga agtggcggtc acagagatga cgccagctct ggtgcgtgcg
     4621 tggtgggccg ggatgggtag gaagcacccg actgcccgcc ggcatgccta caacgtcctc
     4681 cgggcggtga tgaacacagc ggtcgaggac aagctgatcg cagagaaccc gtgccggatc
     4741 gagcagaagg cagccgatga gcgcgacgta gaggcgctga cgcctgagga gctggacatc
     4801 gtcgccgctg agatcttcga gcactaccgg atcgcggcat acatcctggc gtggacgagc
     4861 ctccggttcg gagagctgat cgagcttcgc cgcaaggaca tcgtggacga cggcatgacg
     4921 atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga acaagatcgt cgttggcaac
     4981 gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc ctcacgtcgc ggagatgatc
     5041 cgagcgcaca tgaaggaccg tacgaagatg aacaagggcc ccgaggcatt cctggtgacc
     5101 acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac
     5161 gccaagatcg gtcggccgga actccgcatc cacgacctcc gcgctgtcgg cgctacgttc
     5221 gccgctcagg caggtgcgac gaccaaggag ctgatggccc gtctcggtca cacgactcct
     5281 aggatggcga tgaagtacca gatggcgtct gaggcccgcg acgaggctat cgctgaggcg
     5341 atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag
     5401 tcctaaagag gggggtttct tgtcagtacg cgaagaacca cgcctggccg cgagcgccag
     5461 caccgccgct ctgtgcggag acctgggcac cagccccgcc gccgccagga gcattgccgt
     5521 tcccgccaga aatctagacg gtgaccacaa cgcgcccgct ttgatcgggg acgtcgaaat
     5581 attggatcgt cggcaccgtc acggccgtgg gaggcggcac gatccgcgac gtgatgatcg
     5641 gccgcatccc cacggtgctg cgcagtgagc tctacgccat cccggcgttg atctgtgcgt
     5701 tcgcacgcac aggcccggtg tgagaagggt ctctgacgag cgggagaact ccctatcag
//
