LOCUS       sequence-31548-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Tg-UBC (TgTwinScan_2218)
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(624..642)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      638..685
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaattccctc tgaaataatt ttgtttactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcagag aaaacttgta tttccagggc gcgctgaaac
      121 gcatcaacaa ggaactcaac gacttgagca aagacccacc cacgaactgc tctgcaggac
      181 ccgtcggaga tgacatgttt cactggcaag ctaccatcat gggtcctgaa gacagtccgt
      241 acagcggagg tgtgtttttc ttgaacatcc acttcccgag cgactatccc ttcaaacccc
      301 cgaaagtcaa tttcacaacg aagatctacc accccaacat caacagccaa ggcgcaattt
      361 gcttggatat tctgaaggac cagtggagtc ccgcactcac catctccaaa gttctcctct
      421 ccatctcttc gctcctcaca gaccccaacc ctgatgaccc gctggtgccg gagattgcgc
      481 atttgtacaa gtcagatcgg atgcgatacg accagacggc tcgggaatgg tcgcagaagt
      541 atgcttgatg acgaagcttg cggccgcact cgaggatccg gctgctaaca aagcccgaaa
      601 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      661 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatcc
//
