LOCUS       sequence-31551-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Py-OAT
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     281..300
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     384..403
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        384..402
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    403..427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             442..464
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             483..500
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             510..527
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttacaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
       61 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
      121 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
      181 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
      241 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
      301 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
      361 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
      421 caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca
      481 gccatcatca tcatcatcac agcagcggcc tggttccgcg tggtagtatg gaatttgtca
      541 aagacttaaa aacaccagaa gattatatca acaacgaatt gaaatatgga gctcacaatt
      601 atgatcccat tcctgttgta ttaaaaagag ctaagggtgt atttgtttat gatgttaatg
      661 ataaaagata ctacgatttt ttatctgcat attcatcagt taaccaagga cattgtcacc
      721 caaatatatt aaatgctatg ataaatcaag ctaagaactt aacaatttgt agtagagcct
      781 tttttagtgt acctttagga atatgtgaaa gatatcttac aaacttactt ggttatgata
      841 aagtgttaat gatgaatact ggagcagaag ctaacgaaac tgcatataaa ttatgtagaa
      901 aatgggggat atg
//
