LOCUS       sequence-31552-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pf-PPWD1
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(516..536)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(549..566)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             585..607
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    622..646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(646..665)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(647..665)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(749..768)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agccggatcc tcgagtgcgg ccgcaagctt cgtcatcaat tattaatttt tatatttaat
       61 attttgatat cctctaaagg cttatccctt ttatccgtac gaaccttttc tatatccaaa
      121 acaattttac ttccttgggt tactttacca aaaactgtgt gtttaaaatc gagccaagga
      181 catgggacag tggttataaa aaactgggat ccattagtat ttggaccaca gtttgccata
      241 gataccataa aaggtttgga atgattaaga tggtcaaaga attcatcttc aaattcattt
      301 ccccaaatac tttcaccacc tgtgccatca cctgaaggat ctcccgtttg gaccataaaa
      361 tgtttgataa ctcgatggaa aatgcaatta ttataatatc cgtttataga atgaacagag
      421 aaattttgta ctgttttttt gcattcttta taaaaaagtg aaatatgtat atctcccatg
      481 gttgtgtaaa taatagcact tttaggagta tttttgccct ggaaatacaa gttttctctg
      541 ccgctgctgt gatgatgatg atgatggctg ctgcccatgg tatatctcct tcttaaagtt
      601 aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg
      661 tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc
      721 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      781 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      841 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
      901 ctc
//
