LOCUS       sequence-31555-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Pv-OMPDC (2GUU)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     280..299
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     383..402
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        383..401
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    402..426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             441..463
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             482..499
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             509..526
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tccaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
       61 cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca
      121 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
      181 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
      241 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
      301 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
      361 cgagatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
      421 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacca tgggcagcag
      481 ccatcatcat catcatcaca gcagcggcct ggttccgcgt ggtagtaatt tgaaaataaa
      541 gctacaaaag aggagggacg aagtgaatac atgcctgtgc atcgggctgg acccggatga
      601 agctgacata aagtccttca tgcagagtga gaagcaaaat ggctaccaga gtgtcaaaaa
      661 aaacctaagc aatagtggta gtagtagtag tagtagtaat agtagtagtg gcaagggcga
      721 actgttcgcc ccacaaatgg ggggccaaat gctactcgca gaaaccccac caaaggaggc
      781 acaagaaaag gacgaatttt tttacttctt caatcacttc tgcttttaca taatcaatga
      841 gacgaaggaa tacgccctgg catacaaaat gaatttcgcc ttttacctcc catatggttc
      901 cctc
//
