LOCUS       sequence-315575-        9125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of TKFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Orozco JM, Krawczyk PA, Scaria SM, Cangelosi AL, Chan SH, Kunchok T,
            Lewis CA, Sabatini DM
  TITLE     Dihydroxyacetone phosphate signals glucose availability to mTORC1.
  JOURNAL   Nat Metab. 2020 Sep;2(9):893-901. doi: 10.1038/s42255-020-0250-5. 
            Epub 2020 Jul 27.
  PUBMED    32719541
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-020-0250-5"; journalName: "Nat Metab"; date: 
            "2020-09"; volume: "2"; issue: "9"; pages: "893-901"
COMMENT     Sequence Label: FLAG-TKFC in pLJM60
FEATURES             Location/Qualifiers
     source          1..9125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     355..374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     608..625
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    742..763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(778..783,784..801,802..808)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    816..832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     821..843
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     840..856
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     840..856
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     875..895
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        877..895
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        923..1149
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1150..1330
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1377..1502
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1999..2232
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2417..2461
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2768..2971
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2922..2942
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2968..2992
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3202..3220
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3202..3219
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3233..3252
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3311..3334
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5141..5258
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5307..5817
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5736..5754
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5839..6438
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5839..5858)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6335..6355
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6566..6799
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6871..6992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6908..6927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6962..6981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7032..7167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7187..7206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7188..7206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7216..7233)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7216..7232)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7225..7247)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7374..7829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7461..7480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7671..7692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7855..7959
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7960..8028,8029..8820)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8178..8197)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8991..9125,1..454)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
       61 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      121 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      181 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      241 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      301 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      361 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      421 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      481 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      541 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      601 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
      661 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      721 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      781 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      841 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
      901 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
      961 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     1021 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     1081 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     1141 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     1201 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     1261 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     1321 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     1381 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     1441 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     1501 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     1561 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1621 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     1681 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     1741 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     1801 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     1861 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1921 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1981 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     2041 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     2101 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     2161 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     2221 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     2281 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     2341 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     2401 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     2461 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     2521 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2581 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gaccccatat
     2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     3001 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     3061 ccgggaacgg tgcattggaa cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta
     3121 tagagtctat aggcccaccc ccttggcttc gttagaacgc ggctacaatt aatacataac
     3181 cttatgtatc atacacatac gatttaggtg acactataga ataacatcca ctttgccttt
     3241 ctctccacag gtgtccactc ccaggtccaa ctgcacctcg gttctatcga ttgaattcac
     3301 catggaaatg gactacaagg atgacgatga caagggtggt ggtggtggtg cgtcgacaat
     3361 gacctccaag aagctggtga actcggtggc tggctgtgct gatgacgctc ttgctggcct
     3421 ggtggcctgc aaccccaacc tgcagctcct gcagggccac cgcgtggccc tccgttctga
     3481 cctggacagc ctcaagggcc gggtggcact gctgtcgggt gggggctctg gccatgagcc
     3541 tgcccatgct ggtttcatag ggaaggggat gctgactggg gtcatcgcgg gagctgtgtt
     3601 cacctccccg gcagtgggca gcatcctggc agccatcagg gccgtggccc aggccggcac
     3661 agtggggacg ctccttatcg tgaagaacta cactggggat cggctcaact tcggcctggc
     3721 ccgggagcag gcccgggctg aaggcatccc ggtggagatg gtggtgattg gggacgacag
     3781 cgccttcact gtcctgaaga aggcaggccg gcgggggctg tgcggcaccg tgcttataca
     3841 caaggtggca ggtgctctgg ctgaggctgg tgtggggctg gaggagatcg caaagcaggt
     3901 gaacgtggtc accaaggcca tgggtaccct gggggtgagc ttatccccct gcagcgtccc
     3961 tggttccaaa cccaccttcg agctctcagc cgacgaggtg gagctgggcc tggggatcca
     4021 cggggaagct ggtgtgcgcc ggataaagat ggcaaccgcc gatgagattg tgaaactcat
     4081 gctcgaccac atgacaaaca ccaccaacgc gtcccatgtg cctgtgcagc ccggctcctc
     4141 agttgtgatg atggtcaaca acctgggtgg cctgtcattc ctggaactgg gcatcatagc
     4201 cgacgctacc gtccgctccc tggagggccg cggggtgaag attgcccgtg ccctggtggg
     4261 caccttcatg tcagcactgg agatgcctgg catttctctc accctcctgc tggtggatga
     4321 gcctctcctg aaactgatag atgctgaaac cactgcagca gcctggccta acgtggctgc
     4381 agtctccatt actgggcgga agcggagccg ggtagcccct gccgagcccc aggaggcccc
     4441 tgattccact gctgcaggag gctcagcctc gaagcggatg gcgctggtgc tggaacgggt
     4501 gtgcagcact ctcctgggcc tggaggaaca cctgaatgcc ctggaccggg ctgctggtga
     4561 cggcgactgt ggcaccaccc acagccgtgc ggccagagca atccaggagt ggctgaagga
     4621 gggcccaccc cctgccagcc ctgcccagct gctctccaag ttgtctgttc tgctcctgga
     4681 gaagatggga ggctcatctg gggcgctcta tggcctgttc ctgactgcgg ctgcacagcc
     4741 cctgaaggcc aagaccagcc tcccagcctg gtctgctgcc atggatgccg gcctggaagc
     4801 catgcagaag tatggcaagg ctgctccagg ggacaggact atgctggatt ctctgtgggc
     4861 agcggggcag gagctccaag cctggaagag cccaggagct gatctgttac aagtcctgac
     4921 caaagcagtc aagagtgccg aagctgcagc cgaggccacc aagaatatgg aagctggagc
     4981 cggaagagcc agttatatca gctcagcacg gctggagcag ccagaccccg gggcggtggc
     5041 agctgctgcc atcctccggg ccatcttgga ggtcttgcag agctaggcgg ccgcagtgag
     5101 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     5161 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     5221 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     5281 gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct
     5341 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
     5401 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
     5461 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
     5521 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
     5581 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
     5641 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
     5701 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
     5761 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
     5821 gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5881 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5941 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6001 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6061 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6121 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6181 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6241 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6301 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6361 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6421 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     6481 cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6541 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6601 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6661 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6721 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6781 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6841 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     6901 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6961 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7021 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7081 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7141 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     7201 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7261 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     7321 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     7381 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7441 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7501 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     7561 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     7621 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     7681 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     7741 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     7801 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     7861 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7921 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7981 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8041 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8101 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8161 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8221 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8281 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8341 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8401 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8461 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     8521 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8581 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8641 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8701 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8761 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8821 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8881 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8941 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9001 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9061 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9121 cagat
//
