LOCUS       sequence-315579-        6053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0311:Dendra
FEATURES             Location/Qualifiers
     source          1..6053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1997..2020
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2277..3077
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3202..3790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3691..3710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgtctgcccg gcccgagtct gctccgcacg tgcggcgagt
      121 gctgctgctg tgctggcgcg acaccggcca cccgcagggc ggcggcagcg aggcgtacgt
      181 gcagcgcatc ggcgcatacc tggcaggccg cggtgtcgac gtcacgctgc gcacggcgcg
      241 ctatcccggt tcggcgcgca ccgaggtggt cgacggcgtg cgcatcagcc gtggtggcgg
      301 gccctacacc gtctacatct gggccggtct ggcgatggtg ctcgcgcgca tagggctggg
      361 gccgctgcgc cgcgcgcggc ccgacgtggt gatcgacacc cagaacgggt tgccgttcct
      421 cgcgcggttc gcgttcggcc ggcgcgtcgc ggtgctcgtg caccactgcc accgcgagtt
      481 gtggcccgtc gccggtcctg tcatgggtcg tatcggctgg ttcgtggagt cgtggctgtc
      541 gccgcggctg caccggcgca accagtacgt cacggtgtca ctgccgtcgg cccgtgacct
      601 caatgaactc ggtgtcgatt cacagcggat cgcggtggtg cgcaacggac tcgacgaggc
      661 acccgcgacc accctgaagc tgccccgctc ggagaccccg cgcctggtgg tgttgtcacg
      721 gctggtgccg cacaaacaga tcgaggatgc gctggaagcc gtcgcgcaac tgcgcaccga
      781 gatgcctgat gtgcacctgg acatcctcgg cgacggctgg tggcgggaac gcctggtcga
      841 acacgccgaa ctgctgggca tcaccgacgc ggtgaccttc cacggtcacg tcgacgagga
      901 caccaaacat cgtgtactgc aacgcagttg ggtgcacgtg ctgccgtcgc gcaaagaagg
      961 ctggggtctg gcggtcaccg aggccgcgca gcacgccgtt cccaccatcg gctaccgctc
     1021 gtcgggcggg ctgacggact ccgtggtcga cggggtcacc ggcctgctcg tcgacgaccg
     1081 tgacggtctg gtcgcggggc tgcgccagtt ggtgtccgat cccgtgctgc gcacgcaact
     1141 gggcagcaag gcgcagaccc gcagcgacga gttctcgtgg gcgcagagcg ccgacgccat
     1201 gcggacggta ctggaatcgg tccgcgcggg ccgatacctc agcggcctgg tcaagcttgg
     1261 tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt
     1321 gcatatggag ggcaacgtga acggtcacgc cttcgtgatc gagggggagg gtaagggtaa
     1381 gccttacgaa gggacccaga ccgccaacct caccgtcaag gagggcgccc ctctcccatt
     1441 ctcgtacgat atcctcacta ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc
     1501 cgaggatatc cctgactact tcaagcagag cttcccggag ggatactcct gggagcggac
     1561 gatgaccttc gaagacaagg gtatctgtac gatccgcagc gacatctcgc tggaagggga
     1621 ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat
     1681 gcagaagaag accctcaagt gggagccgag cacggagaag ctgcacgttc gggacggcct
     1741 cctggtcggc aacatcaaca tggcgctgct gctggaaggc ggcggccact acctatgcga
     1801 cttcaagacc acgtacaaag cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga
     1861 ccaccgcatc gaaatcctgg ggaacgattc ggactacaac aaggtcaagt tgtacgagca
     1921 cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga
     1981 gaacctgtat ttccaggact acaaggacga cgacgacaag tgatttaaat aaccccttgg
     2041 ggcctctaaa cgggtcttga ggggtttttt gttaactagc gtacgatcga attcctgcag
     2101 ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg
     2161 ctgaaaatgc cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata
     2221 agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt
     2281 catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc
     2341 agcttctcaa ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta
     2401 gcgtccggcc cctcgaagat gggccacttg gactgatcga ggccctgcgt gctacgctgg
     2461 gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc
     2521 ctgccacgtc gcccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc
     2581 caaatgtaaa gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc
     2641 agatcatctc tgatccattg cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg
     2701 tccatgaact cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc
     2761 tgcatcttgc cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc
     2821 ttcaggatgg caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct
     2881 ttgccttggc ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc
     2941 atgcctttgc tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg
     3001 gccgagatcc gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg
     3061 ctcgccagtc gattggctga gctcatgagc ggagaacgag atgacgttgg aggggcaagg
     3121 tcgcgctgat tgctggggca acacggggga tccactagtt ccatgagcgt cagaccccgt
     3181 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3241 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3301 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3361 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3421 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3481 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3541 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3601 aagcgccacg cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3661 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3721 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3781 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3841 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3901 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg
     3961 tacccgggga tcctctagag tcgaccacca agggcaccat ctctgcttgg gccaccccgt
     4021 tggccgcagc cagctcgctg agagccgtga acgacagggc gaacgccagc ccgccgacgg
     4081 cgagggttcc gaccgctgca actcccggtg caaccttgtc ccggtctatt ctcttcactg
     4141 caccagctcc aatctggtgt gaatgcccct cgtctgttcg cgcaggcggg gggctctatt
     4201 cgtttgtcag catcgaaagt agccagatca gggatgcgtt gcaaccgcgt atgcccaggt
     4261 cagaagagtc gcacaagagt tgcagacccc tggaaagaaa aatggccaga gggcgaaaac
     4321 accctctgac cagcggagcg ggcgacggga atcgaacccg cgtagctagt ttggaagaat
     4381 gggtgtctgc cgaccacata tgggccggtc aagataggtt tttaccccct ctcggctgca
     4441 tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca
     4501 ggagttgcaa cccggtagcc ttgttcacga cgagaggaga cctagttggc acgtcgcgga
     4561 tggggatcgc tgaagactca gcgcagcggg aggatccaag cctcatacgt caacccgcag
     4621 gacggtgtga ggtactacgc gctgcagacc tacgacaaca agatggacgc cgaagcctgg
     4681 ctcgcgggcg agaagcggct catcgagatg gagacctgga cccctccaca ggaccgggcg
     4741 aagaaggcag ccgccagcgc catcacgctg gaggagtaca cccggaagtg gctcgtggag
     4801 cgcgacctcg cagacggcac cagggatctg tacagcgggc acgcggagcg ccgcatctac
     4861 ccggtgctag gtgaagtggc ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg
     4921 gccgggatgg gtaggaagca cccgactgcc cgccggcatg cctacaacgt cctccgggcg
     4981 gtgatgaaca cagcggtcga ggacaagctg atcgcagaga acccgtgccg gatcgagcag
     5041 aaggcagccg atgagcgcga cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc
     5101 gctgagatct tcgagcacta ccggatcgcg gcatacatcc tggcgtggac gagcctccgg
     5161 ttcggagagc tgatcgagct tcgccgcaag gacatcgtgg acgacggcat gacgatgaag
     5221 ctccgggtgc gccgtggcgc ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag
     5281 accgtccggt cgaagcgtcc tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg
     5341 cacatgaagg accgtacgaa gatgaacaag ggccccgagg cattcctggt gaccacgacg
     5401 cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag
     5461 atcggtcggc cggaactccg catccacgac ctccgcgctg tcggcgctac gttcgccgct
     5521 caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac tcctaggatg
     5581 gcgatgaagt accagatggc gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc
     5641 aagctggcca agacctcctg aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa
     5701 agaggggggt ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc
     5761 cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc aggagcattg ccgttcccgc
     5821 cagaaatcta gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg aaatattgga
     5881 tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg atcggccgca
     5941 tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt gcgttcgcac
     6001 gcacaggccc ggtgtgagaa gggtctctga cgagcgggag aactccctat cag
//
