LOCUS       sequence-315607-        5081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0559:Dendra
FEATURES             Location/Qualifiers
     source          1..5081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1025..1048
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1305..2105
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2230..2818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2719..2738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggcacaggg aactgtgaaa tggttcaacg gcgaaaaggg
      121 cttcggcttc atcgcccctg atggcggcgc tccggacgtc ttcgtccact actccgagat
      181 cagcggcagc ggtttccgct cgctcgagga gaatcagcgc gtcgagttcg agatcacgca
      241 gggtgccaag ggcccccagg cagttggcgt taccgcggtc aagcttggtg cggccgcggg
      301 caacacgcct ggcatcaatc tgatcaagga ggacatgcgt gtcaaggtgc atatggaggg
      361 caacgtgaac ggtcacgcct tcgtgatcga gggggagggt aagggtaagc cttacgaagg
      421 gacccagacc gccaacctca ccgtcaagga gggcgcccct ctcccattct cgtacgatat
      481 cctcactacc gcggtgcatt acgggaaccg cgtgttcacc aagtaccccg aggatatccc
      541 tgactacttc aagcagagct tcccggaggg atactcctgg gagcggacga tgaccttcga
      601 agacaagggt atctgtacga tccgcagcga catctcgctg gaaggggact gtttcttcca
      661 gaacgtccgt ttcaagggga ccaactttcc gccgaacggc ccggtcatgc agaagaagac
      721 cctcaagtgg gagccgagca cggagaagct gcacgttcgg gacggcctcc tggtcggcaa
      781 catcaacatg gcgctgctgc tggaaggcgg cggccactac ctatgcgact tcaagaccac
      841 gtacaaagcg aagaaggtcg tccagctgcc cgacgcgcac ttcgtggacc accgcatcga
      901 aatcctgggg aacgattcgg actacaacaa ggtcaagttg tacgagcacg ctgtggcacg
      961 ctatagcccg ctcccgtcgc aggtctggtt cgaaggcggc ggcggggaga acctgtattt
     1021 ccaggactac aaggacgacg acgacaagtg atttaaataa ccccttgggg cctctaaacg
     1081 ggtcttgagg ggttttttgt taactagcgt acgatcgaat tcctgcagct cacggtaact
     1141 gatgccgtat ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg
     1201 gcctttgaat gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca
     1261 ccgactattt gcaacagtgc cgttgatcgt gctatgatcg actgatgtca tcagcggtgg
     1321 agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct catcggtcag cttctcaacc
     1381 ttggggttac ccccggcggt gtgctgctgg tccacagctc cttccgtagc gtccggcccc
     1441 tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc tacgctgggt ccgggaggga
     1501 cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc
     1561 ccgttacacc ggaccttgga gttgtctctg acacattctg gcgcctgcca aatgtaaagc
     1621 gcagcgccca tccatttgcc tttgcggcag cggggccaca ggcagagcag atcatctctg
     1681 atccattgcc cctgccacct tactcgcctg caagcccggt cgcccgtgtc catgaactcg
     1741 atgggcaggt acttctcctc ggcgtgggac acgatgccaa cacgacgctg catcttgccg
     1801 agttgatggc aaaggttccc tatggggtgc cgagacactg caccattctt caggatggca
     1861 agttggtacg cgtcgattat ctcgagaatg accactgctg tgagcgcttt gccttggcgg
     1921 acaggtggct caaggagaag agccttcaga aggaaggtcc agtcggtcat gcctttgctc
     1981 ggttgatccg ctcccgcgac attgtggcga cagccctggg tcaactgggc cgagatccgt
     2041 tgatcttcct gcatccgcca gaggcgggat gcgaagaatg cgatgccgct cgccagtcga
     2101 ttggctgagc tcatgagcgg agaacgagat gacgttggag gggcaaggtc gcgctgattg
     2161 ctggggcaac acgggggatc cactagttcc atgagcgtca gaccccgtag aaaagatcaa
     2221 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2281 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2341 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2401 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2461 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2521 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2581 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2641 tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2701 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2761 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2821 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2881 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2941 taccgctcgc cgcagccgaa cgaccgagcg caacgcgtgc ggccgcggta cccggggatc
     3001 ctctagagtc gaccaccaag ggcaccatct ctgcttgggc caccccgttg gccgcagcca
     3061 gctcgctgag agccgtgaac gacagggcga acgccagccc gccgacggcg agggttccga
     3121 ccgctgcaac tcccggtgca accttgtccc ggtctattct cttcactgca ccagctccaa
     3181 tctggtgtga atgcccctcg tctgttcgcg caggcggggg gctctattcg tttgtcagca
     3241 tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat gcccaggtca gaagagtcgc
     3301 acaagagttg cagacccctg gaaagaaaaa tggccagagg gcgaaaacac cctctgacca
     3361 gcggagcggg cgacgggaat cgaacccgcg tagctagttt ggaagaatgg gtgtctgccg
     3421 accacatatg ggccggtcaa gataggtttt taccccctct cggctgcatc ctctaagtgg
     3481 aaagaaattg caggtcgtag aagcgcgttg aagcctgaga gttgcacagg agttgcaacc
     3541 cggtagcctt gttcacgacg agaggagacc tagttggcac gtcgcggatg gggatcgctg
     3601 aagactcagc gcagcgggag gatccaagcc tcatacgtca acccgcagga cggtgtgagg
     3661 tactacgcgc tgcagaccta cgacaacaag atggacgccg aagcctggct cgcgggcgag
     3721 aagcggctca tcgagatgga gacctggacc cctccacagg accgggcgaa gaaggcagcc
     3781 gccagcgcca tcacgctgga ggagtacacc cggaagtggc tcgtggagcg cgacctcgca
     3841 gacggcacca gggatctgta cagcgggcac gcggagcgcc gcatctaccc ggtgctaggt
     3901 gaagtggcgg tcacagagat gacgccagct ctggtgcgtg cgtggtgggc cgggatgggt
     3961 aggaagcacc cgactgcccg ccggcatgcc tacaacgtcc tccgggcggt gatgaacaca
     4021 gcggtcgagg acaagctgat cgcagagaac ccgtgccgga tcgagcagaa ggcagccgat
     4081 gagcgcgacg tagaggcgct gacgcctgag gagctggaca tcgtcgccgc tgagatcttc
     4141 gagcactacc ggatcgcggc atacatcctg gcgtggacga gcctccggtt cggagagctg
     4201 atcgagcttc gccgcaagga catcgtggac gacggcatga cgatgaagct ccgggtgcgc
     4261 cgtggcgctt cccgcgtggg gaacaagatc gtcgttggca acgccaagac cgtccggtcg
     4321 aagcgtcctg tgacggttcc gcctcacgtc gcggagatga tccgagcgca catgaaggac
     4381 cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga ccacgacgca gggcaaccgg
     4441 ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct acgccaagat cggtcggccg
     4501 gaactccgca tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg
     4561 acgaccaagg agctgatggc ccgtctcggt cacacgactc ctaggatggc gatgaagtac
     4621 cagatggcgt ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa gctggccaag
     4681 acctcctgaa acgcaaaaag cccccctccc aaggacactg agtcctaaag aggggggttt
     4741 cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg ctctgtgcgg
     4801 agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca gaaatctaga
     4861 cggtgaccac aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc gtcggcaccg
     4921 tcacggccgt gggaggcggc acgatccgcg acgtgatgat cggccgcatc cccacggtgc
     4981 tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg
     5041 tgtgagaagg gtctctgacg agcgggagaa ctccctatca g
//
