LOCUS       sequence-315696-        8177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-off lentivirus construct all-in-one vector, with Blast 
            resistance, for expression of TPI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Orozco JM, Krawczyk PA, Scaria SM, Cangelosi AL, Chan SH, Kunchok T,
            Lewis CA, Sabatini DM
  TITLE     Dihydroxyacetone phosphate signals glucose availability to mTORC1.
  JOURNAL   Nat Metab. 2020 Sep;2(9):893-901. doi: 10.1038/s42255-020-0250-5. 
            Epub 2020 Jul 27.
  PUBMED    32719541
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-020-0250-5"; journalName: "Nat Metab"; date: 
            "2020-09"; volume: "2"; issue: "9"; pages: "893-901"
COMMENT     Sequence Label: TPI in pCW57_tTA_Blast
FEATURES             Location/Qualifiers
     source          1..8177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..352
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    46..64
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    82..100
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    224..242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     325..349
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1140..1650
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1569..1587
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1660..2052
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2062..2115
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2116..2733,2734..2739,2740..2775,2776..2778,2779..2814,
                     2815..2817,2818..2853,2854..2862)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(2902..2922)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3320..3331)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3536..3769
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3841..3962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3878..3897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3932..3951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4002..4137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4137..4208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4209..4277,4278..5069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4427..4446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5240..5828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5729..5748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5982..5999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6116..6137
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6152..6157,6158..6175,6176..6182)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6190..6206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6195..6217
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6214..6230
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6214..6230
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6249..6269
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6251..6269
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6297..6523
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6524..6704
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6751..6876
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7369..7602
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7787..7831
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8044..8161
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acagcagaga tccactttgg ccgcgaatcg atatgtcgag tttactccct atcagtgata
       61 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
      121 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      181 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
      241 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
      301 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagaat
      361 tggctagcgt cgacaccatg gcgccctcca ggaagttctt cgttggggga aactggaaga
      421 tgaacgggcg gaagcagagt ctgggggagc tcatcggcac tctgaacgcg gccaaggtgc
      481 cggccgacac cgaggtggtt tgtgctcccc ctactgccta tatcgacttc gcccggcaga
      541 agctagatcc caagattgct gtggctgcgc agaactgcta caaagtgact aatggggctt
      601 ttactgggga gatcagccct ggcatgatca aagactgcgg agccacgtgg gtggtcctgg
      661 ggcactcaga gagaaggcat gtctttgggg agtcagatga gctgattggg cagaaagtgg
      721 cccatgctct ggcagaggga ctcggagtaa tcgcctgcat tggggagaag ctagatgaaa
      781 gggaagctgg catcactgag aaggttgttt tcgagcagac aaaggtcatc gcagataacg
      841 tgaaggactg gagcaaggtc gtcctggcct atgagcctgt gtgggccatt ggtactggca
      901 agactgcaac accccaacag gcccaggaag tacacgagaa gctccgagga tggctgaagt
      961 ccaacgtctc tgatgcggtg gctcagagca cccgtatcat ttatggaggc tctgtgactg
     1021 gggcaacctg caaggagctg gccagccagc ctgatgtgga tggcttcctt gtgggtggtg
     1081 cttccctcaa gcccgaattc gtggacatca tcaatgccaa acaatgagaa ttcggatccg
     1141 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     1201 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     1261 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     1321 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     1381 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     1441 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     1501 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     1561 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     1621 gttgaccgaa tcaccgacct ctctccccag caattcacca tggttccttt gtctcaagaa
     1681 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
     1741 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
     1801 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
     1861 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
     1921 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
     1981 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
     2041 gtgtgggagg gccccggtgc cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa
     2101 gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaactc tgctctggaa
     2161 ttactcaatg aagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     2221 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     2281 gcaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg
     2341 caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc acatcgcgac
     2401 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     2461 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     2521 gccgtgggcc actttacact gggctgcgta ttggaggatc aggagcatca agtagcaaaa
     2581 gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca agcaattgag
     2641 ctgttcgacc atcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     2701 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg ccgacgccct tgacgatttt
     2761 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     2821 gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc
     2881 aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2941 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     3001 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     3061 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     3121 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3181 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3241 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     3301 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3361 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3421 ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca
     3481 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     3541 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     3601 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     3661 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     3721 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     3781 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     3841 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3901 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3961 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     4021 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4081 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt
     4141 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4201 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4261 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4321 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4381 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4441 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4501 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4561 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4621 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4681 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4741 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4801 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4861 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4921 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4981 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5041 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5101 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5161 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5221 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5281 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5341 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5401 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5461 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5521 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5581 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5641 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5701 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5761 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5821 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5881 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5941 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6001 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6061 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6121 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6181 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     6241 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     6301 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     6361 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     6421 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     6481 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     6541 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6601 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6661 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     6721 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     6781 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     6841 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     6901 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     6961 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7021 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7081 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7141 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7201 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     7261 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     7321 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     7381 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     7441 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     7501 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     7561 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     7621 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     7681 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     7741 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     7801 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     7861 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     7921 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     7981 ccacctccca accccgaggg gacaattctc gacctcgaga caaatggcag tattcatcca
     8041 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     8101 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     8161 tcgggtttat tacaggg
//
