LOCUS       sequence-31582-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ICBP90
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             349..366
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(419..437)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      433..480
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      517..969
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(604..623)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     814..835
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggttc cgcgtggtag tatgtggatc caggttcgga ccatggacgg
      121 gaggcagacc cacacggtgg actcgctgtc caggctgacc aaggtggagg agctgaggcg
      181 gaagatccag gagctgttcc acgtggagcc aggcctgcag aggctgttct acaggggcaa
      241 acagatggag gacggccata ccctcttcga ctacgaggtc cgcctgaatg acaccatcca
      301 gctcctggtc cgccagagct gatgacgaag cttgcggccg cactcgagca ccaccaccac
      361 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      421 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      481 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      541 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      601 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      661 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      721 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      781 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      841 cactcaaccc tatctcggtc tattcttttg atttataagg gantttgccg atttcggcct
      901 attggttaaa aatgagctga tttaacaaaa tttaacgcga attttaacaa atattaacgc
      961 ttacaattta
//
