LOCUS       sequence-316010-       13521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast test vector of pPPED.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13521)
  AUTHORS   Wang L, Kaya HB, Zhang N, Rai R, Willmann MR, Carpenter SCD, Read 
            AC, Martin F, Fei Z, Leach JE, Martin GB, Bogdanove AJ
  TITLE     Spelling Changes and Fluorescent Tagging With Prime Editing Vectors 
            for Plants.
  JOURNAL   Front Genome Ed. 2021 Mar 4;3:617553. doi: 
            10.3389/fgeed.2021.617553. eCollection 2021.
  PUBMED    34713247
REFERENCE   2  (bases 1 to 13521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Genome Ed."; date: "2021-03-4"; volume: "3:617553. doi"; pages: " 
            10.3389/fgeed.2021.617553. eCollection 2021"
COMMENT     Sequence Label: pPPEDs
FEATURES             Location/Qualifiers
     source          1..13521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        901..1577
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1514..1534
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1629..1649
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1656..4169,4170..4172,4173..5756)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7944..7964
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8031..8283
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8324..8340)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8539..8663
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(8700..8705,8706..8723,8724..8730)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8784..9443
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8850..8869)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9785..10090
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9969..9988
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10131..10255)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    10282..10306
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        complement(11336..11354)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11375..11391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11375..11391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11388..11410)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11399..11415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11423..11429,11430..11447,11448..11453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11468..11489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11606..11623)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11777..12365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11857..11876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12536..13327,13328..13396))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13159..13178
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13397..13501)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
      721 atccactagt gagtcgtatt acaattcatg actctcttaa ggtagccaaa atcgatatct
      781 agggataaca gggtaatcga tcactagaat tcggcgcgcc gggcccaaca tggtggagca
      841 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat
      901 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
      961 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
     1021 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
     1081 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     1141 ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
     1201 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
     1261 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
     1321 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
     1381 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     1441 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     1501 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     1561 tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc tcgaccgcta
     1621 gcatggcacc taagaaaaag aggaaggttg gtattgataa gaagtactct atcggactcg
     1681 atatcggaac taactctgtg ggatgggctg tgatcaccga tgagtacaag gtgccatcta
     1741 agaagttcaa ggttctcgga aacaccgata ggcactctat caagaaaaac cttatcggtg
     1801 ctctcctctt cgattctggt gaaactgctg aggctaccag actcaagaga accgctagaa
     1861 gaaggtacac cagaagaaag aacaggatct gctacctcca agagatcttc tctaacgaga
     1921 tggctaaagt ggatgattca ttcttccaca ggctcgaaga gtcattcctc gtggaagaag
     1981 ataagaagca cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg gcataccacg
     2041 agaagtaccc tactatctac cacctcagaa agaagctcgt tgattctact gataaggctg
     2101 atctcaggct catctacctc gctctcgctc acatgatcaa gttcagagga cacttcctca
     2161 tcgagggtga tctcaaccct gataactctg atgtggataa gttgttcatc cagctcgtgc
     2221 agacctacaa ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg
     2281 ctatcctctc tgctaggctc tctaagtcaa gaaggcttga gaacctcatt gctcagctcc
     2341 ctggtgagaa gaagaacgga cttttcggaa acttgatcgc tctctctctc ggactcaccc
     2401 ctaacttcaa gtctaacttc gatctcgctg aggatgcaaa gctccagctc tcaaaggata
     2461 cctacgatga tgatctcgat aacctcctcg ctcagatcgg agatcagtac gctgatttgt
     2521 tcctcgctgc taagaacctc tctgatgcta tcctcctcag tgatatcctc agagtgaaca
     2581 ccgagatcac caaggctcca ctctcagctt ctatgatcaa gagatacgat gagcaccacc
     2641 aggatctcac acttctcaag gctcttgtta gacagcagct cccagagaag tacaaagaga
     2701 ttttcttcga tcagtctaag aacggatacg ctggttacat cgatggtggt gcatctcaag
     2761 aagagttcta caagttcatc aagcctatcc tcgagaagat ggatggaacc gaggaactcc
     2821 tcgtgaagct caatagagag gatcttctca gaaagcagag gaccttcgat aacggatcta
     2881 tccctcatca gatccacctc ggagagttgc acgctatcct tagaaggcaa gaggatttct
     2941 acccattcct caaggataac agggaaaaga ttgagaagat tctcaccttc agaatccctt
     3001 actacgtggg acctctcgct agaggaaact caagattcgc ttggatgacc agaaagtctg
     3061 aggaaaccat caccccttgg aacttcgaag aggtggtgga taagggtgct agtgctcagt
     3121 ctttcatcga gaggatgacc aacttcgata agaaccttcc aaacgagaag gtgctcccta
     3181 agcactcttt gctctacgag tacttcaccg tgtacaacga gttgaccaag gttaagtacg
     3241 tgaccgaggg aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg
     3301 atctcttgtt caagaccaac agaaaggtga ccgtgaagca gctcaaagag gattacttca
     3361 agaaaatcga gtgcttcgat tcagttgaga tttctggtgt tgaggatagg ttcaacgcat
     3421 ctctcggaac ctaccacgat ctcctcaaga tcattaagga taaggatttc ttggataacg
     3481 aggaaaacga ggatatcttg gaggatatcg ttcttaccct caccctcttt gaagatagag
     3541 agatgattga agaaaggctc aagacctacg ctcatctctt cgatgataag gtgatgaagc
     3601 agttgaagag aagaagatac actggttggg gaaggctctc aagaaagctc attaacggaa
     3661 tcagggataa gcagtctgga aagacaatcc ttgatttcct caagtctgat ggattcgcta
     3721 acagaaactt catgcagctc atccacgatg attctctcac ctttaaagag gatatccaga
     3781 aggctcaggt ttcaggacag ggtgatagtc tccatgagca tatcgctaac ctcgctggat
     3841 ctcctgcaat caagaaggga atcctccaga ctgtgaaggt tgtggatgag ttggtgaagg
     3901 tgatgggaag gcataagcct gagaacatcg tgatcgaaat ggctagagag aaccagacca
     3961 ctcagaaggg acagaagaac tctagggaaa ggatgaagag gatcgaggaa ggtatcaaag
     4021 agcttggatc tcagatcctc aaagagcacc ctgttgagaa cactcagctc cagaatgaga
     4081 agctctacct ctactacctc cagaacggaa gggatatgta tgtggatcaa gagttggata
     4141 tcaacaggct ctctgattac gatgttgatg ctatcgtgcc acagtcattc ttgaaggatg
     4201 attctatcga taacaaggtg ctcaccaggt ctgataagaa caggggtaag agtgataacg
     4261 tgccaagtga agaggttgtg aagaaaatga agaactattg gaggcagctc ctcaacgcta
     4321 agctcatcac tcagagaaag ttcgataact tgactaaggc tgagagggga ggactctctg
     4381 aattggataa ggcaggattc atcaagaggc agcttgtgga aaccaggcag atcactaagc
     4441 acgttgcaca gatcctcgat tctaggatga acaccaagta cgatgagaac gataagttga
     4501 tcagggaagt gaaggttatc accctcaagt caaagctcgt gtctgatttc agaaaggatt
     4561 tccaattcta caaggtgagg gaaatcaaca actaccacca cgctcacgat gcttacctta
     4621 acgctgttgt tggaaccgct ctcatcaaga agtatcctaa gctcgagtca gagttcgtgt
     4681 acggtgatta caaggtgtac gatgtgagga agatgatcgc taagtctgag caagagatcg
     4741 gaaaggctac cgctaagtat ttcttctact ctaacatcat gaatttcttc aagaccgaga
     4801 ttaccctcgc taacggtgag atcagaaaga ggccactcat cgagacaaac ggtgaaacag
     4861 gtgagatcgt gtgggataag ggaagggatt tcgctaccgt tagaaaggtg ctctctatgc
     4921 cacaggtgaa catcgttaag aaaaccgagg tgcagaccgg tggattctct aaagagtcta
     4981 tcctccctaa gaggaactct gataagctca ttgctaggaa gaaggattgg gaccctaaga
     5041 aatacggtgg tttcgattct cctaccgtgg cttactctgt tctcgttgtg gctaaggttg
     5101 agaagggaaa gagtaagaag ctcaagtctg ttaaggaact tctcggaatc actatcatgg
     5161 aaaggtcatc tttcgagaag aacccaatcg atttcctcga ggctaaggga tacaaagagg
     5221 ttaagaagga tctcatcatc aagctcccaa agtactcact cttcgaactc gagaacggta
     5281 gaaagaggat gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt gctctcccat
     5341 ctaagtacgt taactttctt tacctcgctt ctcactacga gaagttgaag ggatctccag
     5401 aagataacga gcagaagcaa cttttcgttg agcagcacaa gcactacttg gatgagatca
     5461 tcgagcagat ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac ctcgataagg
     5521 tgttgtctgc ttacaacaag cacagagata agcctatcag ggaacaggca gagaacatca
     5581 tccatctctt cacccttacc aacctcggtg ctcctgctgc tttcaagtac ttcgatacaa
     5641 ccatcgatag gaagagatac acctctacca aagaagtgct cgatgctacc ctcatccatc
     5701 agtctatcac tggactctac gagactagga tcgatctctc acagctcggt ggtgatctca
     5761 gtggtgggag cagtggtgga tcatcaggga gtgagactcc aggtacaagt gaaagcgcta
     5821 ctcctgaatc ttcaggaggt tcctcaggag gaagttctac tcttaacatc gaggacgagt
     5881 atcgtctcca cgagaccagc aaggaacctg atgtatctct aggatcaact tggctcagtg
     5941 atttccccca ggcatgggca gaaacaggtg gcatgggttt ggctgtacgt caagcaccac
     6001 ttataattcc tctaaaagca acaagcaccc ccgtcagtat taagcagtac ccaatgagtc
     6061 aggaggctag attgggcatt aagccacaca tacagagact tttagatcaa ggcattctgg
     6121 taccctgtca gtccccctgg aacacaccac tgctgcctgt taagaaacca ggaaccaacg
     6181 attaccgacc agttcaggac ttgcgagaag tgaacaaaag ggtcgaggat atccacccta
     6241 ccgtgcctaa tccatacaac cttttatctg gccttcctcc aagccatcaa tggtacaccg
     6301 ttctagattt gaaagatgca ttcttttgtc tgcgactcca ccccacctca cagccccttt
     6361 tcgccttcga gtggagagac cctgaaatgg gtatcagcgg ccaacttact tggactaggt
     6421 taccccaggg atttaaaaac agtccaaccc tattcaatga ggcattgcac agggacctgg
     6481 cagattttcg tatacaacac cccgatctaa tattgttaca atacgtggac gaccttctcc
     6541 tagctgcaac aagcgagtta gattgtcagc aaggaacaag ggctcttttg caaacactag
     6601 gaaacttggg gtatcgtgct agtgccaaaa aagctcagat ttgtcaaaag caggtaaagt
     6661 acttagggta tttactaaag gaaggtcaga gatggttaac cgaagcccga aaggaaactg
     6721 taatggggca accaactcct aaaactccca gacaactcag ggagttcttg ggcaaagcag
     6781 ggttttgtag acttttcata cccggctttg ctgaaatggc agcacccctg tatcccttga
     6841 caaagcctgg gacactattt aactgggggc cagaccagca gaaggcatac caagaaataa
     6901 agcaagcact actcaccgca cctgccctag gcctgcctga tctgactaaa ccatttgaac
     6961 tcttcgtaga tgagaaacaa ggctacgcta aaggtgtgct gacacaaaaa ttgggaccct
     7021 ggcgacgacc agtcgcctac ctcagcaaga aattagaccc cgtggccgcc ggttggccac
     7081 catgcttacg aatggtggca gcaatcgccg tcctgacaaa ggatgcaggc aaacttacca
     7141 tgggccaacc tctagtcatc ttagcacccc atgccgttga ggctctcgta aagcagccac
     7201 ctgatagatg gctcagtaac gctcgaatga cccactatca agccctcctc ttggataccg
     7261 acagagtcca gttcggaccc gtagtagcac ttaacccagc aacactgcta cccctccctg
     7321 aggaagggct acaacataac tgcctagaca ttttggccga ggcacatggg acacgtccag
     7381 acttgacaga tcagccctta ccagacgccg accacacatg gtacacagac ggaagttcac
     7441 tcttgcagga gggtcagagg aaggccgggg ctgctgtgac taccgagacc gaagttattt
     7501 gggctaaggc cctaccagcc ggcacctctg cacagagagc agagttaatt gcactaaccc
     7561 aggccctcaa aatggccgaa ggtaaaaaac taaatgtcta tacagattca cgatatgctt
     7621 ttgcaacagc ccatatacat ggcgaaattt ataggaggag ggggtggtta acatcagagg
     7681 gaaaagagat aaaaaacaag gacgaaatcc ttgcattgct aaaggcttta ttccttccca
     7741 agcgtttaag cataatccac tgtcccggtc atcagaaggg tcactccgcc gaggcaagag
     7801 gcaaccgaat ggctgaccaa gccgcaagga aggccgcaat cactgagacc ccagacacct
     7861 ctactctact aatcgaaaac agctcccctt caggtggaag caagagaacc gccgacggca
     7921 gtgaattcga aagcagggct gaccccaaga agaagaggaa ggtgtgaccg cggatatctg
     7981 cagaagctta cgcgtcgacg tctcgaggcc ggccgagctc gaatttcccc gatcgttcaa
     8041 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     8101 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     8161 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     8221 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     8281 atcgggaatt gatatctagg gataacaggg taataagctt ggcactggcc gtcgttttac
     8341 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     8401 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     8461 gcagcctgaa tggcgaatga gcttgagctt ggatcagatt gtcgtttccc gccttcagtt
     8521 tggactctag agttatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
     8581 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     8641 acacaacata tccagtcact atggcggccc ctcgaggggc cgcattaggc accccaggct
     8701 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     8761 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     8821 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     8881 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     8941 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     9001 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     9061 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     9121 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     9181 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     9241 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     9301 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     9361 atgccgtctg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     9421 atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt
     9481 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     9541 gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     9601 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     9661 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     9721 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa cagggactgg
     9781 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     9841 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     9901 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     9961 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
    10021 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
    10081 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
    10141 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
    10201 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttgata
    10261 acagcggaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
    10321 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
    10381 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttaa agtactttaa
    10441 agtactttaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
    10501 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
    10561 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
    10621 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
    10681 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
    10741 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
    10801 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
    10861 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
    10921 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
    10981 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
    11041 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
    11101 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
    11161 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
    11221 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
    11281 gaccgaggcc gacgccctgg cggccgccac cgcggtggag ctccagcttt tgttcccttt
    11341 agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    11401 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    11461 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    11521 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    11581 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    11641 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    11701 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    11761 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    11821 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    11881 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    11941 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    12001 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    12061 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    12121 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    12181 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    12241 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    12301 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    12361 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    12421 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    12481 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    12541 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    12601 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    12661 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    12721 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    12781 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    12841 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    12901 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    12961 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    13021 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    13081 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    13141 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    13201 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    13261 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    13321 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    13381 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    13441 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    13501 gcacatttcc ccgaaaagtg c
//
