LOCUS       sequence-316072-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site 
            to insert a CaTCH barcoding library or individual barcode sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, 
            Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber 
            J, Obenauf AC
  TITLE     Isolating live cell clones from barcoded populations using 
            CRISPRa-inducible reporters.
  JOURNAL   Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0.
  PUBMED    32719478
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0."
COMMENT     Sequence Label: CaTCH_empty-backbone
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    154..387
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
       61 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
      121 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
      181 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
      241 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
      301 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
      361 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
      421 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
      481 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
      541 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattca agcagaagac
      601 ggcatacgag atgtgactgg agttcagacg tgtgctcttc cgatctattg gaattagata
      661 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
      721 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
      781 tgaatagagt taggcaggga tattcaccat tatcgtt
//
