LOCUS       sequence-316076-        5735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0371:Dendra
FEATURES             Location/Qualifiers
     source          1..5735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1679..1702
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1959..2759
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2884..3472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3373..3392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggccgatac acagaccacc cagcccagtg ggctgaagaa
      121 catggcgcgg gccgtggtcg gcgccctgcc gttcgtcacc cgcagcgaga gcctgcccaa
      181 gcgcaccgtc acggtcgacg acctgacgat cgatccggcc aacgtcgccg agtacgccca
      241 ggtgaccggc ctgcggttcg gcgacgccgt gccgctcacc tacccgttca cgctgacgtt
      301 cccggcggtg atgtcgctgg tgaccggcct cgacttcccg ttcgcggcaa tgggttcggt
      361 gcacatcgag aaccacatca cgcagtaccg gccgatcgcg gtgaccgaca ccgtcggcgt
      421 cgcggtgcac gccgagaacc tgcgtgagca ccgccgcggc ctgctcgtcg acatcgtcac
      481 cgacgtcaag gtgggcaacg agttggcctg gcaccaggtg acgacgttcc tgcatcagca
      541 gcgcacgagc ctgtccggcg agcccaagcc gccaccgcag aagcagccga aactgccgcc
      601 gcccaacgcg gtcctgcgca tcacgccggg gcagatccgg cactacgccg cggtcggtgg
      661 ggatcacaat ccgatccaca ccaacgcgat cgcggccaag ctgttcggct tccccaccgt
      721 gatcgctcac gggatgttca gcgccgcagc cgttctggcc aacatcgaag ggcaactttc
      781 cgacgccgtg aagcactcgg tgcggttcgc caaaccggtg gtgctgcccg ccaaggccgc
      841 gctctacgtc gagcgcgacg ccgacggctg ggagctcacg ctgcggcacc tgaccaaggg
      901 gtatccgcac ctgaccgcga cggtcacccc gctcaagctt ggtgcggccg cgggcaacac
      961 gcctggcatc aatctgatca aggaggacat gcgtgtcaag gtgcatatgg agggcaacgt
     1021 gaacggtcac gccttcgtga tcgaggggga gggtaagggt aagccttacg aagggaccca
     1081 gaccgccaac ctcaccgtca aggagggcgc ccctctccca ttctcgtacg atatcctcac
     1141 taccgcggtg cattacggga accgcgtgtt caccaagtac cccgaggata tccctgacta
     1201 cttcaagcag agcttcccgg agggatactc ctgggagcgg acgatgacct tcgaagacaa
     1261 gggtatctgt acgatccgca gcgacatctc gctggaaggg gactgtttct tccagaacgt
     1321 ccgtttcaag gggaccaact ttccgccgaa cggcccggtc atgcagaaga agaccctcaa
     1381 gtgggagccg agcacggaga agctgcacgt tcgggacggc ctcctggtcg gcaacatcaa
     1441 catggcgctg ctgctggaag gcggcggcca ctacctatgc gacttcaaga ccacgtacaa
     1501 agcgaagaag gtcgtccagc tgcccgacgc gcacttcgtg gaccaccgca tcgaaatcct
     1561 ggggaacgat tcggactaca acaaggtcaa gttgtacgag cacgctgtgg cacgctatag
     1621 cccgctcccg tcgcaggtct ggttcgaagg cggcggcggg gagaacctgt atttccagga
     1681 ctacaaggac gacgacgaca agtgatttaa ataacccctt ggggcctcta aacgggtctt
     1741 gaggggtttt ttgttaacta gcgtacgatc gaattcctgc agctcacggt aactgatgcc
     1801 gtatttgcag taccagcgta cggcccacag aatgatgtca cgctgaaaat gccggccttt
     1861 gaatgggttc atgtgcagct ccatcagcaa aaggggatga taagtttatc accaccgact
     1921 atttgcaaca gtgccgttga tcgtgctatg atcgactgat gtcatcagcg gtggagtgca
     1981 atgtcgtgca atacgaatgg cgaaaagccg agctcatcgg tcagcttctc aaccttgggg
     2041 ttacccccgg cggtgtgctg ctggtccaca gctccttccg tagcgtccgg cccctcgaag
     2101 atgggccact tggactgatc gaggccctgc gtgctacgct gggtccggga gggacgctcg
     2161 tcatgccctc gtggtcaggt ctggacgacg agccgttcga tcctgccacg tcgcccgtta
     2221 caccggacct tggagttgtc tctgacacat tctggcgcct gccaaatgta aagcgcagcg
     2281 cccatccatt tgcctttgcg gcagcggggc cacaggcaga gcagatcatc tctgatccat
     2341 tgcccctgcc accttactcg cctgcaagcc cggtcgcccg tgtccatgaa ctcgatgggc
     2401 aggtacttct cctcggcgtg ggacacgatg ccaacacgac gctgcatctt gccgagttga
     2461 tggcaaaggt tccctatggg gtgccgagac actgcaccat tcttcaggat ggcaagttgg
     2521 tacgcgtcga ttatctcgag aatgaccact gctgtgagcg ctttgccttg gcggacaggt
     2581 ggctcaagga gaagagcctt cagaaggaag gtccagtcgg tcatgccttt gctcggttga
     2641 tccgctcccg cgacattgtg gcgacagccc tgggtcaact gggccgagat ccgttgatct
     2701 tcctgcatcc gccagaggcg ggatgcgaag aatgcgatgc cgctcgccag tcgattggct
     2761 gagctcatga gcggagaacg agatgacgtt ggaggggcaa ggtcgcgctg attgctgggg
     2821 caacacgggg gatccactag ttccatgagc gtcagacccc gtagaaaaga tcaaaggatc
     2881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3001 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3301 ggggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3601 tcgccgcagc cgaacgaccg agcgcaacgc gtgcggccgc ggtacccggg gatcctctag
     3661 agtcgaccac caagggcacc atctctgctt gggccacccc gttggccgca gccagctcgc
     3721 tgagagccgt gaacgacagg gcgaacgcca gcccgccgac ggcgagggtt ccgaccgctg
     3781 caactcccgg tgcaaccttg tcccggtcta ttctcttcac tgcaccagct ccaatctggt
     3841 gtgaatgccc ctcgtctgtt cgcgcaggcg gggggctcta ttcgtttgtc agcatcgaaa
     3901 gtagccagat cagggatgcg ttgcaaccgc gtatgcccag gtcagaagag tcgcacaaga
     3961 gttgcagacc cctggaaaga aaaatggcca gagggcgaaa acaccctctg accagcggag
     4021 cgggcgacgg gaatcgaacc cgcgtagcta gtttggaaga atgggtgtct gccgaccaca
     4081 tatgggccgg tcaagatagg tttttacccc ctctcggctg catcctctaa gtggaaagaa
     4141 attgcaggtc gtagaagcgc gttgaagcct gagagttgca caggagttgc aacccggtag
     4201 ccttgttcac gacgagagga gacctagttg gcacgtcgcg gatggggatc gctgaagact
     4261 cagcgcagcg ggaggatcca agcctcatac gtcaacccgc aggacggtgt gaggtactac
     4321 gcgctgcaga cctacgacaa caagatggac gccgaagcct ggctcgcggg cgagaagcgg
     4381 ctcatcgaga tggagacctg gacccctcca caggaccggg cgaagaaggc agccgccagc
     4441 gccatcacgc tggaggagta cacccggaag tggctcgtgg agcgcgacct cgcagacggc
     4501 accagggatc tgtacagcgg gcacgcggag cgccgcatct acccggtgct aggtgaagtg
     4561 gcggtcacag agatgacgcc agctctggtg cgtgcgtggt gggccgggat gggtaggaag
     4621 cacccgactg cccgccggca tgcctacaac gtcctccggg cggtgatgaa cacagcggtc
     4681 gaggacaagc tgatcgcaga gaacccgtgc cggatcgagc agaaggcagc cgatgagcgc
     4741 gacgtagagg cgctgacgcc tgaggagctg gacatcgtcg ccgctgagat cttcgagcac
     4801 taccggatcg cggcatacat cctggcgtgg acgagcctcc ggttcggaga gctgatcgag
     4861 cttcgccgca aggacatcgt ggacgacggc atgacgatga agctccgggt gcgccgtggc
     4921 gcttcccgcg tggggaacaa gatcgtcgtt ggcaacgcca agaccgtccg gtcgaagcgt
     4981 cctgtgacgg ttccgcctca cgtcgcggag atgatccgag cgcacatgaa ggaccgtacg
     5041 aagatgaaca agggccccga ggcattcctg gtgaccacga cgcagggcaa ccggctgtcg
     5101 aagtccgcgt tcaccaagtc gctgaagcgt ggctacgcca agatcggtcg gccggaactc
     5161 cgcatccacg acctccgcgc tgtcggcgct acgttcgccg ctcaggcagg tgcgacgacc
     5221 aaggagctga tggcccgtct cggtcacacg actcctagga tggcgatgaa gtaccagatg
     5281 gcgtctgagg cccgcgacga ggctatcgct gaggcgatgt ccaagctggc caagacctcc
     5341 tgaaacgcaa aaagcccccc tcccaaggac actgagtcct aaagaggggg gtttcttgtc
     5401 agtacgcgaa gaaccacgcc tggccgcgag cgccagcacc gccgctctgt gcggagacct
     5461 gggcaccagc cccgccgccg ccaggagcat tgccgttccc gccagaaatc tagacggtga
     5521 ccacaacgcg cccgctttga tcggggacgt cgaaatattg gatcgtcggc accgtcacgg
     5581 ccgtgggagg cggcacgatc cgcgacgtga tgatcggccg catccccacg gtgctgcgca
     5641 gtgagctcta cgccatcccg gcgttgatct gtgcgttcgc acgcacaggc ccggtgtgag
     5701 aagggtctct gacgagcggg agaactccct atcag
//
