LOCUS       sequence-316101-        9318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018259
FEATURES             Location/Qualifiers
     source          1..9318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             325..347
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      1559..1568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2022..2042
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2043..2066
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    2092..2148
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2149..2166)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2149..2165)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2158..2180)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2374..2393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2493..2515
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2553..2571)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3357..4556
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(4787..5935)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(6207..6578)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6611..6720
                     /note="incP origin of transfer"
     rep_origin      6873..7504
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      7967..8555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8456..8475
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8709..8726
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8843..8864
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8879..8884,8885..8902,8903..8909)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8917..8933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8922..8944
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8941..8957
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8941..8957
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcaacgcaaa atgctgaatt tagcaggcaa gtttcctgat aaagggccct gtgagtgcga
       61 cattcgacgc gcgaaagcga gttgacggcg cccgaaattt tcttagagtt cgttcaagct
      121 tcggtttcgg ggagccgcta agggatgcgg acccagccgg gtaagtcgcc gggaactatg
      181 gaataatcct aatttctgtg cggcaggcgc gagctgccgg agcgaatatc atccctgtcg
      241 caaacagcag gtcaatcaac ggtgggtagc gggctgctat ccagcggcgg ataagacttg
      301 tcggaggaat cctctagaaa taattttgtt taactttaag aaggagatat accgtgctga
      361 ccttcggctg catgcggagg gaacgccccg ctatctgtgc ggccgggggg gagctggcct
      421 cttcgggacc cgcgcggatc gactccctcc cggcgccggg cgcggcggcc gtcccggacg
      481 tcgactggcc gacctacggc ggcaacgacg ccggccagcg gtactcgctg ctcgcccaga
      541 tcgactccgg caatgtccgc cggctcgtcc cggtctgcct gcaccgcgcc caggtcggtc
      601 tggaaacgca gcagagcacc cccgtcgtcg atggtgacgc cctcctctat accctcccct
      661 acgatgtggt cgtggccatg gacccccgga ccggccggga attgtggcgc tacacgccga
      721 ggctggcccg ggactccctg gtgctgtgct gcggccccca caatcgcgga gtggccgtgg
      781 acggcgcgcg cgtgtacctc gccacactcg atgcgcgact ggtggccctt gaccgtcgca
      841 cgggtgcggt ggcgtgggag gtgcgggtcg ccgagccgat gaagggctac agcttcaccg
      901 cggcgccgct gctggccgac ggcaaggtga tcctcggcac tgccggaggc gaattcggca
      961 tccgaggttg ggtcgacgcc tacgacgcgg gcacggggca gcgcgcctgg cgcttctaca
     1021 cggtgccctc cccagacgag ggcggctggt ggggccgctg ggccgccacc acgccagacg
     1081 gcgaggacct gcatcgcgat ctggcccggg agcgcgccga ttcggctcgg tacgcggatg
     1141 cgtggcgcac aggcggcgga cccgtgtgga tgaccccggc gttcgacgcc gcgctcggcc
     1201 tggtgtttat cgggatcggc aatcccgccc ccgatatcga cgaaagcaag cgccccggcg
     1261 ataacctcta cacgagttcg atcgcggccc tcgacgtgcg aaccggggcc ctgaagtggt
     1321 atcatcagct ggtgccgcac gaccggtggg actacgacgc cgcgaacccg gtggtgctcc
     1381 tcgatgccac cgtaaacggc gccaccgtcc cggctatcgc ccacgcaggc aaggcgggct
     1441 gggtgcatgt gttggatcgc cgcgatggcc gccgactggt gcggaccgag gccctggtgc
     1501 cccagaagaa tctcttcatg tccgcgacgg cggagggcat cgaggtcgcg cccggcggcc
     1561 atggcggcgc aaactgggcc ccgtcggctt atagcccgcg gacccgtctg atgtacgtgc
     1621 tggccaacca cgagccctca gtgttcaaac tcgtccccgc ccccctctcg cagggcgcgc
     1681 agtggttcgg cggggagatg acggtggcga accgcggccg cggcgcgttc ggccgcctca
     1741 acgcggtgga tccctccacc ggcaagatcc gctggcagct caccgagaag aacctgaaaa
     1801 actacagcgg gggtacgctc gcgacggcgg gagacctcgt gttctttggc gacgcggatg
     1861 gctatctcaa cgccgtcgat gcccgcagcg gcgcccggct ctggcgtcac tggacggggc
     1921 gaccgagcat cgacgccgcc ccgatgacgt tcagtcgggg tggccgccag tacgtcgcca
     1981 tcgcgaccca gggcgcgctg gtggccttca ccctccgcgc cgaaaacctc tattttcagg
     2041 gacatcatca ccatcaccat caccactgac agctatgacc atgattacgc caagcttgca
     2101 tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt
     2161 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2221 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2281 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     2341 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2401 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtatgctccc
     2461 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     2521 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     2581 taatgtcatg ataataatgg tttcttagca ccctttctcg gtccttcaac gttcctgaca
     2641 acgagcctcc ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg
     2701 tccggaccgg cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc
     2761 tgttcaacgg tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg
     2821 gccccaacag tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa
     2881 cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt
     2941 cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg
     3001 cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta
     3061 tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag
     3121 tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga
     3181 ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc
     3241 aactttgtca tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag
     3301 tgataaagaa tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga
     3361 aacccaacat acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg
     3421 gcctgattat gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca
     3481 ccgcccacta tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg
     3541 tgctgggcgc gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg
     3601 gcgccactgt cgactacgcc atcatggcga cagcgccttt cctttgggtt ctctatatcg
     3661 ggcggatcgt ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg
     3721 atatcactga tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt
     3781 tcgggatggt cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc
     3841 cgttcttcgc cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc
     3901 cggagtcgca caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt
     3961 cgttccggtg ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca
     4021 tgcaacttgt cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc
     4081 actgggacgc gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg
     4141 cccaggcaat gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc
     4201 tcggaatgat tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga
     4261 tggcgttccc gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag
     4321 caatgttgtc caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg
     4381 cgctcaccag cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt
     4441 ctataacaac gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc
     4501 tgccggcgct gcgtcgcggg ctttggagcg gcgcagggca acgagccgat cgctgatcgt
     4561 ggaaacgata ggcctatgcc atgcgggtca aggcgacttc cggcaagcta tacgcgccct
     4621 agaattgtca attttaatcc tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg
     4681 agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta
     4741 aagcgctggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat
     4801 gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc
     4861 aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga
     4921 ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca
     4981 tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt
     5041 cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct
     5101 tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc
     5161 ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg
     5221 tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg
     5281 tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt
     5341 cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt
     5401 ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca
     5461 gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat
     5521 cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct
     5581 gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg
     5641 tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga
     5701 gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca
     5761 cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg
     5821 tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa
     5881 accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc
     5941 ctccttgcgg gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc
     6001 cgcctggtgc cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga
     6061 ccgtctggcc gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagct
     6121 ccgcgaagtc gctcttcttg atggagcgca tggggacgtg cttggcaatc acgcgcaccc
     6181 cccggccgtt ttagcggcta aaaaagtcat ggctctgccc tcgggcggac cacgcccatc
     6241 atgaccttgc caagctcgtc ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg
     6301 gcatcaccga accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag caggccgccc
     6361 aggcggccca ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata gtccacgacg
     6421 cccgtgattt tgtagccctg gccgacggcc agcaggtagg cctacaggct catgccggcc
     6481 gccgccgcct tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac cttgataggt
     6541 gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg
     6601 ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
     6661 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
     6721 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
     6781 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
     6841 tatgcagcgg aaaagatccg tcgacccttt ccgacgctca ccgggctggt tgccctcgcc
     6901 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
     6961 gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac
     7021 agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat
     7081 gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa
     7141 aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc
     7201 ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca
     7261 cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc
     7321 acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct
     7381 gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc
     7441 ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc
     7501 cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac
     7561 cccaaaaatg gcagccaagc tgaccacttc tgcgctcggc ccttccggct ggctggttta
     7621 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7681 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7741 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7801 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7861 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7921 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7981 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8041 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8101 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8161 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8221 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8281 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8341 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8401 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8461 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8521 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8581 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8641 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8701 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8761 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8821 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8881 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8941 caggaaacag ctatgaccat gattacgcca agctagcttc ccgcttggtc gggccgcttc
     9001 gcgagggccc gttgacgaca acggtgcgat gggtcccggc cccggtcaag acgatgccaa
     9061 tacgttgcga cactacgcct tggcactttt agaattgcct tatcgtcctg ataagaaatg
     9121 tccgaccagc taaagacatc gcgtccaatc aaagcctaga aaatataggc gaagggacgc
     9181 taataagtct ttcataagac cgcgcaaatc taaaaatatc cttagattca cgatgcggca
     9241 cttcggatga cttccgagcg agcctggaac ctcagaaaaa cgtctgagag ataccgcgga
     9301 tctcacacag gaaatttc
//
